Susana Brom Klanner
Genome Engineering Researcher
Associate Professor
Academic history
Name: Susana Brom Klanner (Associate Professor).
Academic history
Undergraduate (B Sc.): Basic Biomedical Research, U.N.A.M. 1980.
Isolation and characterization of mutations in the glnA region of the E. coli K12 chromosome.
.
Masters (M Sc.): Basic Biomedical Research, National Autonomous University of Mexico, 1982. Characterization of mutations in the glnA region of the E. coli K12 chromosome
Ph.D.Basic Biomedical Research, National Autonomous University of Mexico, 1988.
Analisis of symbiotic plasmids in Rhizobium phaseoli.
Initial research position
Assistant professor, Nitrogen Fixation Research Center, National Autonomous University of Mexico, 1983- 1993
Research line
Rhizobium are gram negative bacteria, able to form a symbiotic relationship with the roots of leguminous plants. Specifically, Rhizobium etli interacts with the roots of Phaseolus vulgaris (beans). The genome of the R. etli type strain CFN42 consists of a chromosome and 6 plasmids, varying in size between 180 and 600 Kb. We are interested in:
1- Further analyzing the functions encoded on plasmids. Previously we have found that plasmid encoded information is required for functions such as optimal competitive capacity for nodulation, saprophytic growth, lipopolysaccharide production, and one of the plasmids even seems to contain information which is indispensable for survival.
2- Analyzing the different mechanisms that contribute to symbiotic plasmid transfer, and their regulation.
3- Search for the presence of site-specific recombination systems, similar to the one we found on R. etli CFN42 plasmids; on other Rhizobiacea.
This work will contribute to a better understanding of the role of plasmids in the rhizobial life cycle, through the knowledge of plasmid encoded functions, and of the mechanisms that contribute to the distribution of symbiotic plasmids and the elements and conditions that control these processes.
Contributions
My work has contributed to the description of associated functions in plasmids of Rhizobium. Also, we have found that different strains of Rhizobium etli, share the localization on plasmids of some specific characteristics, independent of their geographic origin. Rhizobium strains from belonging to other species do not share this trait, even if they are able to nodulate bean.
We have described the regulatory mechanism of the conjugative transfer of a cryptic plasmid from R. etli (p42a).
We have studied the transfer capacity of symbiotic plasmid of R. etli CFN42. We have found two different mechanisms, one of them involves the cointegration of the symbiotic with another plasmid through a site specific recombination system. The resolution of the cointegrates sometimes leads to the generation of new symbiotic plasmids.
Present research position
Associate professor, Center of Genomic Sciences, National Autonomous University of Mexico.
Teaching
Undergraduate: 11 courses
Masters: 10 courses
Ph.D.: 7 courses.
Distinctions
* Gabino Barreda medal, for undergraduate studies, National Autonomous University of Mexico, 1980.
* Honorific Mention in Undergraduate professional exam. National Autonomous University of Mexico, 1980.
Honorific Mention in Masters professional exam. National Autonomous University of Mexico, 1982.
* Gabino Barreda medal, for Ph.D. studies, National Autonomous University of Mexico, 1990.
* Sor Juana Inés de la Cruz medal for research contributions, 2005.
Links
Thomson Reuters ResearcherID
OrcID
Scopus
Telephone: (777) 3291691
Email: sbrom@ccg.unam.mx
Publications
Year | Publication | PMID |
---|---|---|
2021 | Castellani, L.G., Luchetti, A., Nilsson, J.F., Pérez-Giménez, J., Wegener, C., Schlüter, A., Pühler, A., Lagares, A., Brom, S., Pistorio, M., Niehaus, K., Torres Tejerizo, G.A.. (2021). "Exopolysaccharide Characterization of Rhizobium Favelukesii LPU83 and Its Role in the Symbiosis With Alfalfa.". Frontiers In Plant Science. 12():-. [doi:10.3389/fpls.2021.642576] | 33643369 |
2021 | Brom, Susana, Grohman, Elisabeth, Llosa, Matxalen. (2021). "Early Career Researcher (ECR)". Plasmid. 113():-. [doi:10.1016/j.plasmid.2020.102526] | 32750387 |
2020 | Bañuelos-Vazquez, L.A., Cazares, D., Rodríguez, S., Cervantes-De La Luz, L., Sánchez-López, R., Castellani, L.G., Tejerizo, G.T., Brom, S.. (2020). "Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42 to Endophytic Bacteria Inside Nodules.". Frontiers in Microbiology. 11():-. [doi:10.3389/fmicb.2020.01752] | 32849381 |
2020 | Bañuelos-Vazquez, L.A., Castellani, L.G., Luchetti, A., Romero, D., Torres Tejerizo, G.A., Brom, S.. (2020). "Role of plant compounds in the modulation of the conjugative transfer of pRet42a". Plos One. 15(8):238218-238218. [doi:10.1371/journal.pone.0238218] | 32845909 |
2019 | Cervantes, L., Miranda-Sánchez, F., Torres Tejerizo, G., Romero, D., Brom, S.. (2019). "Plasmid pSfr64a and the symbiotic plasmid pSfr64b of Sinorhizobium fredii GR64 control each other's conjugative transfer through quorum-sensing elements.". Plasmid. 106():-. [doi:10.1016/j.plasmid.2019.102443] | 31689451 |
2019 | Castellani, Lucas G., Nilsson, Juliet F., Wibberg, Daniel, Schlueter, Andreas, Puehler, Alfred, Brom, Susana, Pistorio, Mariano, Tejerizo, Gonzalo Torres. (2019). "Insight into the structure, function and conjugative transfer of pLPU83a, an accessory plasmid of Rhizobium Favelukesii LPU83". Plasmid. 103():9-16. [doi:10.1016/j.plasmid.2019.03.004] | 30928704 |
2019 | Gonzalez, Victor, Isela Santamaria, Rosa, Bustos, Patricia, Maria Perez-Carrascal, Olga, Vinuesa, Pablo, Juarez, Soledad, Martinez-Flores, Irma, Angel Cevallos, Miguel, Brom, Susana, Martinez-Romero, Esperanza, Romero, David. (2019). "Phylogenomic Rhizobium Species Are Structured by a Continuum of Diversity and Genomic Clusters". Frontiers in Microbiology. 10():-. [doi:10.3389/fmicb.2019.00910] | 31114559 |
2019 | Bañuelos-Vazquez, L.A., Torres Tejerizo, G., Cervantes-De La Luz, L., Girard, L., Romero, D., Brom, S.. (2019). "Conjugative transfer between Rhizobium etli endosymbionts inside the root nodule". Environmental Microbiology. 21(9):3430-3441. [doi:10.1111/1462-2920.14645] | 31037804 |
2017 | Cubillas, C., Miranda-Sánchez, F., González-Sánchez, A., Elizalde, J.P., Vinuesa, P., Brom, S., García-de los Santos, A.. (2017). "A comprehensive phylogenetic analysis of copper transporting P1BATPases from bacteria of the Rhizobiales order uncovers multiplicity, diversity and novel taxonomic subtypes". MICROBIOLOGYOPEN. 6(4):-. [doi:10.1002/mbo3.452] | 28217917 |
2017 | Alfredo Banuelos-Vazquez, Luis, Torres Tejerizo, Gonzalo, Brom, Susana. (2017). "Regulation of conjugative transfer of plasmids and integrative conjugative elements". Plasmid. 91():82-89. [doi:10.1016/j.plasmid.2017.04.002] | 28438469 |
2016 | Hernández-Tamayo, R., Torres-Tejerizo, G., Brom, S., Romero, D.. (2016). "Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli". Bmc Microbiology. 16(1):133-133. [doi:10.1186/s12866-016-0755-y] | 27357704 |
2015 | Torres Tejerizo, G., Bañuelos, L.A., Cervantes, L., Gaytán, P., Pistorio, M., Romero, D., Brom, S.. (2015). "Development of molecular tools to monitor conjugative transfer in Rhizobia". JOURNAL OF MICROBIOLOGICAL METHODS. 117():155-163. [doi:10.1016/j.mimet.2015.08.005] | 26272377 |
2015 | López-Fuentes E, Torres-Tejerizo G, Cervantes L, Brom S. (2015). "Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors". Frontiers in Microbiology. 5():793-793. [doi:10.3389/fmicb.2014.00793] | 25642223 |
2014 | Brom, S., Pistorio, M., Romero, D., Torres-Tejerizo, G.. (2014). "Boundaries for conjugative transfer of rhizobial plasmids: Restraining and releasing factors". Plasticity In Plant-Growth-Promoting And Phytopathogenic Bacteria. 9781461492030():43-54. [doi:10.1007/978-1-4614-9203-0_3] | |
2014 | Tejerizo, G.T., Pistorio, M., Althabegoiti, M.J., Cervantes de la Luz, Laura, Wibberg, D., Schluter, A., Puhler, A., Lagares, A., Romero Camarena, David René, Brom Klanner, Susana. (2014). "Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background". Fems Microbiology Ecology. 88(3):565-578. [doi:10.1111/1574-6941.12325] | 24646299 |
2013 | Hernández Tamayo, Rogelio, Sohlenkamp, Christian, Puente, J.L., Brom Klanner, Susana, Romero Camarena, David René. (2013). "Characterization of IntA, a bidirectional site-specific recombinase required for conjugative transfer of the symbiotic plasmid of Rhizobium etli CFN42". Journal Of Bacteriology. 195(20):4668-4677. [doi:10.1128/JB.00714-13] | 23935046 |
2012 | Tejerizo, G.T., Lozano, L, Gonzalez, V, Bustos, P, Romero, D, Brom, S. (2012). "Draft genome sequence of the bean-nodulating Sinorhizobium fredii strain GR64". Journal Of Bacteriology. 194(24):6978-6978. [doi:10.1128/JB.01882-12] | 23209231 |
2011 | Cervantes de la Luz, Laura, Bustos Arcos, Virginia Patricia, Girard Cuesy, María de Lourdes, Santamaria Gutiérrez, Rosa Isela, Dávila Ramos, José Guillermo, Vinuesa Fleischmann, Pablo, Romero Camarena, David René, Brom Klanner, Susana. (2011). "The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain". Bmc Microbiology. 11(1):1-11. [doi:10.1186/1471-2180-11-149] | 21702991 |
2011 | Landeta Escamilla, Cristina, Dávalos Rodríguez, Araceli Elvira, Cevallos Gaos, Miguel Ángel Carlos, Geiger, Otto, Brom Klanner, Susana, Romero Camarena, David René. (2011). "Plasmids with a chromosome-like role in Rhizobia". Journal Of Bacteriology. 193(6):1317-1326. [doi:10.1128/JB.01184-10] | 21217003 |
2008 | Sepulveda, E., Perez-Mendoza, D., Ramirez-Romero, M. A., Soto, M. J., Lopez-Lara, I. M., Geiger, O., Sanjuan, J., Brom, S., Romero, D.. (2008). "Modulation of conjugative activity of rhizobial symbiotic plasmids by repression and transcriptional interference". FEBS JOURNAL. 275(1):49-49. | |
2008 | Sepulveda, Edgardo, Perez-Mendoza, Daniel, Ramirez-Romero, Miguel A., Soto, Maria J., Lopez-Lara, Isabel M., Geiger, Otto, Sanjuan, Juan, Brom, Susana, Romero, David. (2008). "Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli". Journal Of Bacteriology. 190(12):4189-4197. [doi:10.1128/JB.00041-08] | 18424522 |
2008 | Garcfa-de los Santos, Alejandro, Lopez, Erika, Cubillas, Ciro A., Noel, K. Dale, Brom, Susana, Romero, David. (2008). "Requirement of a plasmid-encoded catalase for survival of Rhizobium etli CFN42 in a polyphenol-rich environment". Applied And Environmental Microbiology. 74(8):2398-2403. [doi:10.1128/AEM.02457-07] | 18310436 |
2007 | Sepulveda, Edgardo, Perez-Mendoza, Daniel, Munoz, Socorro, Ramirez-Romero, Miguel A., Soto, Maria Jose, Cervantes, Laura, Herrera-Cervera, Jose A., Lopez-Lara, Isabel Sabel, Geiger, Otto, Sanuan, Juan, Brom, Susana, Romero, David. (2007). "Regulation of conjugal transfer of the Rhizobium etli Sym plasmid.". Plasmid. 57(2):220-221. | |
2007 | Romero, David, Garcia-De Los Santos, Alejandro, Brom, Susana. (2007). "Essential plasmids in Rhizobium.". Plasmid. 57(2):227-227. | |
2007 | Brom, Susana, Garcia-De Los Santos, Alejandro, Romero, Laura Cervantes David. (2007). "Conjugative transfer of Rhizobium plasmids.". Plasmid. 57(2):220-220. | |
2005 | Perez-Mendoza, D, Sepulveda, E, Pando, V, Munoz, S, Nogales, J, Olivares, J, Soto, MJ, Herrera-Cervera, JA, Romero, D, Brom, S, Sanjuan, J. (2005). "Identification of the rctA gene, which is required for repression of conjugative transfer of rhizobial symbiotic megaplasmids". Journal Of Bacteriology. 187(21):7341-7350. [doi:10.1128/JB.187.21.7341-7350.2005] | 16237017 |
2004 | Brom Klanner, Susana, Girard Cuesy, María de Lourdes, Tun Garrido, Cristina Soleda, García de los Santos, Alejandro, Bustos Arcos, Virginia Patricia, González Zúñiga, Víctor Manuel, Romero Camarena, David René. (2004). "Transfer of the symbiotic plasmid of Rhizobium etli CFN42 requires cointegration with p42a, which may be mediated by site-specific recombination". Journal Of Bacteriology. 186(22):7538-7548. [doi:10.1128/JB.186.22.7538-7548.2004] | 15516565 |
2004 | Sepulveda, Edgardo, Perez-Mendoza, Daniel, Munoz, Socorro, Ramirez-Romero, Miguel A., Soto, Maria Jose, Cervantes, Laura, Herrera-Cervera, Jose A., Lopez-Lara, Isabel Sabel, Geiger, Otto, Sanuan, Juan, Brom, Susana, Romero, David. (2004). "Identification of functional mob regions in Rhizobium etli: Evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d". Journal Of Bacteriology. 186(17):5753-5761. [doi:10.1128/JB.186.17.5753-5761.2004] | 15317780 |
2003 | Vargas, MD, Encarnacion, S, Davalos, A, Reyes-Perez, A, Mora, Y, Garcia-de los Santos, A, Brom, S, Moral, J. (2003). "Only one catalase, katG, is detectable in Rhizobium etli, and is encoded along with the regulator OxyR on a plasmid replicon". MICROBIOLOGY-SGM. 149(5):1165-1176. [doi:10.1099/mic.0.25909-0] | 12724378 |
2003 | Tun-Garrido, C, Bustos, P, Gonzalez, V, Brom, S. (2003). "Conjugative transfer of p42a from Rhizobium etli CFN42, which is required for mobilization of the symbiotic plasmid, is regulated by quorum sensing". Journal Of Bacteriology. 185(5):1681-1692. [doi:10.1128/JB.185.5.1681-1692.2003] | 12591886 |
2002 | Cevallos, MA, Porta, H, Izquierdo, J, Tun-Garrido, C, Garcia-de-los-Santos, A, Davila, G, Brom, S. (2002). "Rhizobium etli CFN42 contains at least three plasmids of the repABC family: A structural and evolutionary analysis". Plasmid. 48(2):104-116. [doi:10.1016/S0147-619X(02)00119-1] | 12383728 |
2002 | Garcia-de los Santos, A, Morales, A, Baldoma, L, Clark, SRD, Brom, S, Yost, CK, Hernandez-Lucas, I, Aguilar, J, Hynes, MF. (2002). "The glcB locus of Rhizobium leguminosarum VF39 encodes an arabinose-inducible malate synthase". Canadian Journal Of Microbiology. 48(10):922-932. [doi:10.1139/w02-091] | 12489782 |
2002 | Brom Klanner, Susana, Girard Cuesy, María de Lourdes, García de los Santos, Alejandro, Sanjuan-Pinilla, J.M., Olivares, J., Sanjuan, J.. (2002). "Conservation of plasmid-encoded traits among bean-nodulating Rhizobium species". Applied And Environmental Microbiology. 68(5):2555-2561. [doi:10.1128/AEM.68.5.2555-2561.2002] | 11976134 |
2000 | Girard Cuesy, María de Lourdes, Brom Klanner, Susana, Dávalos Rodríguez, Araceli Elvira, López, O., Soberón Chávez, Mario, Romero Camarena, David René. (2000). "Differential regulation of fixN-reiterated genes in Rhizobium etli by a novel fixL-fixK cascade". Molecular Plant-Microbe Interactions. 13(12):1283-1292. [doi:10.1094/MPMI.2000.13.12.1283] | 11106020 |
2000 | Brom, S, Garcia-de los Santos, A, Cervantes, L, Palacios, R, Romero, D. (2000). "In Rhizobium etli symbiotic plasmid transfer, nodulation competitivity and cellular growth require interaction among different replicons". Plasmid. 44(1):34-43. [doi:10.1006/plas.2000.1469] | 10873525 |
1999 | Brom Klanner, Susana, Romero Camarena, David René. (1999). "Plasmid biology 98: international symposium on plasmid biology". Plasmid. 41(2):141-177. | 10087219 |
1997 | GarciadelosSantos, A, Brom, S. (1997). "Characterization of two plasmid-borne lps beta loci of Rhizobium etli required for lipopolysaccharide synthesis and for optimal interaction with plants". Molecular Plant-Microbe Interactions. 10(7):891-902. [doi:10.1094/MPMI.1997.10.7.891] | 9304861 |
1996 | Santos, AGD, Brom, S, Romero, D. (1996). "Rhizobium plasmids in bacteria-legume interactions". World Journal Of Microbiology & Biotechnology. 12(2):119-125. [doi:10.1007/BF00364676] | 24415159 |
1995 | ROMERO, D, MARTINEZSALAZAR, J, GIRARD, L, BROM, S, DAVILA, G, PALACIOS, R, FLORES, M, RODRIGUEZ, C. (1995). "Discrete amplifiable regions (amplicons) in the symbiotic plasmid of Rhizobium etli CFN42". Journal Of Bacteriology. 177(4):973-980. [doi:10.1128/jb.177.4.973-980.1995] | 7860608 |
1993 | Flores López, Margarita, Brom Klanner, Susana, Stepkowsky, Tomász, Girard Cuesy, María de Lourdes, Dávila Ramos, José Guillermo, Romero Camarena, David René, Palacios de la Lama, Rafael. (1993). "Gene amplification in Rhizobium: Identification and in vivo cloning of discrete amplifiable DNA regions (amplicons) from Rhizobium leguminosarum biovar phaseoli". PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 90(11):4932-4936. [doi:10.1073/pnas.90.11.4932] | 8506337 |
1992 | BROM, S, DELOSSANTOS, AG, STEPKOWSKI, T, FLORES, M, DAVILA, G, ROMERO, D, PALACIOS, R. (1992). "DIFFERENT PLASMIDS OF Rhizobium-leguminosarum BV phaseoli ARE REQUIRED FOR OPTIMAL SYMBIOTIC PERFORMANCE". Journal Of Bacteriology. 174(16):5183-5189. [doi:10.1128/jb.174.16.5183-5189.1992] | 1644746 |
1991 | GIRARD, MD, FLORES, M, BROM, S, ROMERO, D, PALACIOS, R, DAVILA, G. (1991). "Structural complexity of the symbiotic plasmid of Rhizobium leguminosarum bv. phaseoli". Journal Of Bacteriology. 173(8):2411-2419. [doi:10.1128/jb.173.8.2411-2419.1991] | 2013564 |
1991 | ROMERO, D, BROM, S, MARTINEZSALAZAR, J, GIRARD, MD, PALACIOS, R, DAVILA, G. (1991). "Amplification and deletion of a nod-nif region in the symbiotic plasmid of Rhizobium phaseoli". Journal Of Bacteriology. 173(8):2435-2441. [doi:10.1128/jb.173.8.2435-2441.1991] | 2013567 |
1991 | BROM, S, DELOSSANTOS, AG, DELOURDESGIRARD, M, DAVILA, G, PALACIOS, R, ROMERO, D. (1991). "High-frequency rearrangements in Rhizobium leguminosarum bv. phaseoli plasmids". Journal Of Bacteriology. 173(3):1344-1346. [doi:10.1128/jb.173.3.1344-1346.1991] | 1991727 |
1991 | ROMERO, D, BROM, S, MARTINEZSALAZAR, J, GIRARD, ML, FLORES, M, DURAN, L, DELOSSANTOS, AG, PALACIOS, R, DAVILA, G. (1991). "GEONOMIC INSTABILITY IN Rhizobium - FRIEND OR FOE". Advances In Molecular Genetics Of Plant-Microbe Interactions, Vol 1. 10():211-214. | |
1988 | BROM, S, MARTINEZ, E, DAVILA, G, PALACIOS, R. (1988). "NARROW-HOST-RANGE AND BROAD-HOST-RANGE SYMBIOTIC PLASMIDS OF Rhizobium SPP STRAINS THAT NODULATE Phaseolus-VULGARIS". Applied And Environmental Microbiology. 54(5):1280-1283. [doi:10.1128/AEM.54.5.1280-1283.1988] | 16347637 |
1988 | MARTINEZ, E, FLORES, M, BROM, S, ROMERO, D, DAVILA, G, PALACIOS, R. (1988). "Rhizobium phaseoli: A molecular genetics view". PLANT AND SOIL. 108(1):179-184. [doi:10.1007/BF02370113] | |
1987 | FLORES, M, GONZALEZ, V, BROM, S, MARTINEZ, E, PINERO, D, ROMERO, D, DAVILA, G, PALACIOS, R. (1987). "Reiterated DNA sequences in Rhizobium and Agrobacterium spp.". Journal Of Bacteriology. 169(12):5782-5788. [doi:10.1128/jb.169.12.5782-5788.1987] | 3450286 |
1983 | DAVILA, G, BROM, S, MORA, Y, PALACIOS, R, MORA, J. (1983). "GENETIC AND BIOCHEMICAL-CHARACTERIZATION OF GLUTAMINE-SYNTHETASE FROM NEUROSPORA-CRASSA GLUTAMINE AUXOTROPHS AND THEIR REVERTANTS". Journal Of Bacteriology. 156(3):993-1000. | 6139363 |