Michael Frederick Dunn

Functional Genomics of Prokaryotes Researcher

Associate Professor

Academic history

Michael F. Dunn earned his Bachelors of Science in Biology from Delaware Valley University (Doylestown, PA) in 1985. He worked for two semesters as a student intern in the plant tissue culture laboratory of Dr. Robert Zacharias at the U. S. Department of Agriculture’s Eastern Regional Research Center (ERRC) (Philadelphia, PA) and returned to the ERRC in 1985 to work as a technician in the bacterial phytopathology lab under Dr. William Fett. He began his doctoral work with Dr. Arthur Karr in the Plant Pathology Department at the University of Missouri-Columbia in 1987 and was awarded his Ph.D in 1992 with a thesis entitled “Characterization of an extracellular polysaccharide depolymerase produced by Bradyrhizobium japonicum“. He began his post-doctoral research with Dr. Jamie Mora at the National Autonomous University of Mexico’s Nitrogen Fixation Research Center (CIFN) in 1992. Here he initially focused on studying carbon metabolism (Kreb’s cycle and anaplerotic reactions, the glyoxylate shunt, and polyhydroxybutyrate synthesis) in the nitrogen-fixing bacteria Rhizobium etli and Sinorhizobium meliloti. As an Associate Researcher (1994-96) he focused on biotin metabolism in R. etli and characterized its pyruvate carboxylase, an enzyme that is still used biochemical and structural model for this class of biotin-dependent enzyme. He was a founding member of the Mexican Proteomics Society and collaborated in the first proteomic studies carried out in Mexico during the early years of the 21st Century, studying global metabolic protein expression in R. etli. In 2004, the CIFN became the Center for Genomic Science (CCG) and he became a tenured Research Professor in 2006. He focused on the functional genomics of rhizobial carbon metabolism for the remainder of that decade. In 2009, he was invited to collaborate with the Drs. Graciela Huerta and Karina Navarro of the Colegio de la Frontera Sur, Unidad Tapachula to study antibiotics and degradative enzymes produced by newly isolated bacterial biocontrol agents. Around this time, his interest in rhizobial metabolism shifted from carbon to nitrogen, specifically to studying the genetics and biochemistry of ornithine and arginine synthesis in S. meliloti. His current research is aimed at defining the roles of bioactive compounds called polyamines, which are derived from ornithine in S. meliloti. He and his students have discovered key points in the regulation of ornithine and polyamine biosynthesis, and are currently extending their biochemical characterization of the polyamine biosynthesis pathways and determining the effect of these compounds on gene transcription.



Telephone: (777) 120 4773
Email: mike@ccg.unam.mx


2021Hernández, V.M., Arteaga, A., Dunn, M.F.. (2021). "Diversity, properties and functions of bacterial arginases.". Fems Microbiology Reviews. 45(6):-. [doi:10.1093/femsre/fuab034]34160574
2021López-Chávez, M.Y., Alvarez-Legorreta, T., Infante-Mata, D., Dunn, M.F., Guillén-Navarro, K.. (2021). "Glyphosate-remediation potential of selected plant species in artificial wetlands". SCIENCE OF THE TOTAL ENVIRONMENT. 781():-. [doi:10.1016/j.scitotenv.2021.146812]
2020Becerra-Rivera, V.A., Arteaga, A., Leija, A., Hernández, G., Dunn, M.F.. (2020). "Polyamines produced by Sinorhizobium meliloti Rm8530 contribute to symbiotically relevant phenotypes ex planta and to nodulation efficiency on alfalfa.". MICROBIOLOGY-SGM. 166(3):278-287. [doi:10.1099/mic.0.000886]31935179
2020Ide, A.A., Hernández, V.M., Medina-Aparicio, L., Carcamo-Noriega, E., Girard, L., Hernández-Lucas, I., Dunn, M.F.. (2020). "Genetic regulation, biochemical properties and physiological importance of arginase from Sinorhizobium meliloti". MICROBIOLOGY-SGM. 166(5):484-497. [doi:10.1099/mic.0.000909]32216867
2019Taboada, H., Dunn, M.F., Meneses, N., Vargas-Lagunas, C., Buchs, N., Andrade-Domínguez, A., Encarnación, S.. (2019). "Qualitative changes in proteins contained in outer membrane vesicles produced by Rhizobium etli grown in the presence of the nod gene inducer naringenin.". Archives Of Microbiology. 201(9):1173-1194. [doi:10.1007/s00203-019-01682-4]31172251
2019Taboada, H., Meneses, N., Dunn, M.F., Vargas-Lagunas, C., Buchs, N., Castro-Mondragón, J.A., Heller, M., Encarnación, S.. (2019). "Proteins in the periplasmic space and outer membrane vesicles of Rhizobium etli CE3 grown in minimal medium are largely distinct and change with growth phase.". MICROBIOLOGY-SGM. 165(6):638-650. [doi:10.1099/mic.0.000720]30358529
2019Becerra-Rivera, Victor A., Dunn, Michael F.. (2019). "Polyamine biosynthesis and biological roles in Rhizobia". Fems Microbiology Letters. 366(7):-. [doi:10.1093/femsle/fnz084]31062028
2018Becerra-Rivera, V.A., Bergström, E., Thomas-Oates, J., Dunn, M.F.. (2018). "Polyamines are required for normal growth in Sinorhizobium meliloti". MICROBIOLOGY-SGM. 164(4):600-613. [doi:10.1099/mic.0.000615]29619919
2017Dunn, M. F.. (2017). "Rhizobial Amino Acid Metabolism: Polyamine Biosynthesis and Functions". Handbook Of Microbial Metabolism Of Amino Acids. ():352-370.
2017Rosas-Pérez, T, Dunn, Michael Frederick, Martínez-Romero, E. (2017). "The Symbiome of Llaveia Cochineals (Hemiptera: Coccoidea: Monophlebidae) Includes a Gammaproteobacterial Cosymbiont Sodalis TME1 and the Known Candidatus Walczuchella monophlebidarum". Insect Physiology and Ecology. ():115-134. [doi:10.5772/66442]
2017Gamez-Reyes, Aurora, Becerra-Lobato, Noe, Augusto Ramirez-Trujillo, Jose, Martinez-Romero, Esperanza, Dunn, Michael F., Hernandez-Lucas, Ismael. (2017). "The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems". Annals of Microbiology. 67(3):263-273. [doi:10.1007/s13213-017-1257-3]
2017Meneses, N., Taboada, H., Dunn, M.F., Vargas, M.d.C., Buchs, N., Heller, M., Encarnación, S.. (2017). "The naringenin-induced exoproteome of Rhizobium etli CE3". Archives Of Microbiology. 199(5):737-755. [doi:10.1007/s00203-017-1351-8]28255691
2016Augusto Ramirez-Trujillo, Jose, Dunn, Michael F., Suarez-Rodriguez, Ramon, Hernandez-Lucas, Ismael. (2016). "The Sinorhizobium meliloti glyoxylate cycle enzyme isocitrate lyase (aceA) is required for the utilization of poly-ß-hydroxybutyrate during carbon starvation". Annals of Microbiology. 66(2):921-924. [doi:10.1007/s13213-015-1131-0]
2016Moreno Castillo, Benjamin, Dunn, Michael F., Guillen Navarro, Karina, Holguin Melendez, Francisco, Hernandez Ortiz, Magdalena, Encarnacion Guevara, Sergio, Huerta Palacios, Graciela. (2016). "Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella Fijiensis Morelet". World Journal Of Microbiology & Biotechnology. 32(3):1-12. [doi:10.1007/s11274-015-1993-0]26873555
2015Frederick Dunn, Michael. (2015). "Key roles of microsymbiont amino acid metabolism in Rhizobia-legume interactions". Critical Reviews in Microbiology. 41(4):411-451. [doi:10.3109/1040841X.2013.856854]24601835
2015Ingrid Gutierrez-Roman, Martha, Holguin-Melendez, Francisco, Dunn, Michael F., Guillen-Navarro, Karina, Huerta-Palacios, Graciela. (2015). "Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella Fijiensis, causal agent of black Sigatoka in banana (Musa spp.)". Biocontrol. 60(4):565-572. [doi:10.1007/s10526-015-9655-6]
2015Hernandez, Victor M., Girard, Lourdes, Hernandez-Lucas, Ismael, Vazquez, Alejandra, Ortiz-Ortiz, Catalina, Diaz, Rafael, Dunn, Michael F.. (2015). "Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021". MICROBIOLOGY-SGM. 161(8):1671-1682. [doi:10.1099/mic.0.000122]26271664
2014Gutiérrez-Roman, M.I., Dunn, Michael Frederick, Tinoco-Valencia, R., Holguin-Melendez, F., Huerta-Palacios, G., Guillen-Navarro, K.. (2014). "Potentiation of the synergistic activities of chitinases chiA, chiB and chiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP)". World Journal Of Microbiology & Biotechnology. 30(1):33-42. [doi:10.1007/s11274-013-1421-2]23824666
2013Gutiérrez-Román, M. I., Dunn, Michael Frederick, Tinoco-Valencia, F., Holguín-Meléndez, K., Guillen Navarro, Griselda Karina, Huerta-Palacios, G.. (2013). "Antifungal activity of chitinases (chiA, chiC and chiB) produced by Serratia marcescens CFFSUR-B2 against Mycosphaerella Fijiensis causal agent of black sigatoka in banana (Musa spp.)". Acta Phytopathol. 43():96-.
2012Gutiérrez-Roman, M.I., Holguin-Melendez, F., Bello-Mendoza, R., Guillén-Navarro, K., Dunn, Michael Frederick, Huerta-Palacios, G.. (2012). "Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens". World Journal Of Microbiology & Biotechnology. 28(1):145-153. [doi:10.1007/s11274-011-0803-6]22806790
2011Dunn, Michael F.. (2011). "Anaplerotic function of phosphoenolpyruvate carboxylase in Bradyrhizobium japonicum USDA110". Current Microbiology. 62(6):1782-1788. [doi:10.1007/s00284-011-9928-y]21479798
2009Dunn, M. F., Ramirez-Trujillo, J. A., Hernandez-Lucas, I.. (2009). "Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis". MICROBIOLOGY-SGM. 155(10):3166-3175. [doi:10.1099/mic.0.030858-0]19684068
2008Martinez-Aguilar, Lourdes, Diaz, Rafael, Pena-Cabriales, Juan Jose, Estrada-de los Santos, Paulina, Dunn, Michael F., Caballero-Mellado, Jesus. (2008). "Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species". Applied And Environmental Microbiology. 74(14):4574-4579. [doi:10.1128/AEM.00201-08]18502926
2008Vargas, M. del C., Villalobos, M. A., Diaz, R., Flores, M. A., Contreras, S., Encarnacion, S., Mora, Y., Dunn, M. F., Girard, L., Mora, J.. (2008). "COMPARATIVE ANALYSIS OF argC PROTEIN EXPRESSION IN Rhizobium SPECIES". Biological Nitrogen Fixation: Towards Poverty Alleviation Through Sustainable Agriculture. 42():239-240.
2007Ramirez-Trujillo, J. A., Encarnacion, S., Salazar, E., de los Santos, A. Garcia, Dunn, M. F., Emerich, D. W., Calva, E., Hernandez-Lucas, I.. (2007). "Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcB". Journal Of Bacteriology. 189(16):5875-5884. [doi:10.1128/JB.00385-07]17526694
2005Guillen-Navarro, K, Araiza, G, Garcia-de los Santos, A, Mora, Y, Dunn, MF. (2005). "The Rhizobium etli bioMNY operon is involved in biotin transport". Fems Microbiology Letters. 250(2):209-219. [doi:10.1016/j.femsle.2005.07.020]16099603
2005Guillen-Navarro, K, Encarnacion, S, Dunn, MF. (2005). "Biotin biosynthesis, transport and utilization in Rhizobia". Fems Microbiology Letters. 246(2):159-165. [doi:10.1016/j.femsle.2005.04.020]15899401
2005Guillen-Navarro, K, Araiza, G, Dunn, MF. (2005). "Molecular characterization of genes involved in biotin uptake in Rhizobium etli CE3". Biological Nitrogen Fixation, Sustainable Agriculture And The Environment. 41():243-243.
2004Dunn, MF, Araiza, G, Mora, J. (2004). "Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A". MICROBIOLOGY-SGM. 150(2):399-406. [doi:10.1099/mic.0.26779-0]14766918
2003Encarnacion, S, Guzman, Y, Dunn, MF, Hernandez, M, Vargas, MD, Mora, J. (2003). "Proteome analysis of aerobic and fermentative metabolism in Rhizobium etli CE3". Proteomics. 3(6):1077-1085. [doi:10.1002/pmic.200300427]12833533
2002Encarnacion, S, del Vargas, M, Dunn, MF, Davalos, A, Mendoza, G, Mora, Y, Mora, J. (2002). "aniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli". Journal Of Bacteriology. 184(8):2287-2295. [doi:10.1128/JB.184.8.2287-2295.2002]11914361
2002Dunn, MF, Araiza, G, Encarnacion, S, del Vargas, M, Mora, J. (2002). "Effect of aniA (carbon flux regulator) and phaC (poly-ß-hydroxybutyrate synthase) mutations on pyruvate metabolism in Rhizobium etli". Journal Of Bacteriology. 184(8):2296-2299. [doi:10.1128/JB.184.8.2296-2299.2002]11914362
2001Dunn, MF, Araiza, G, Finan, TM. (2001). "Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021". Archives Of Microbiology. 176(5):355-363. [doi:10.1007/s002030100336]11702077
1998Dunn, MF. (1998). "Tricarboxylic acid cycle and anaplerotic enzymes in Rhizobia". Fems Microbiology Reviews. 22(2):105-123. [doi:10.1016/S0168-6445(98)00010-2]9729766
1997Dunn, MF, Araiza, G, Cevallos, MA, Mora, J. (1997). "Regulation of pyruvate carboxylase in Rhizobium etli". Fems Microbiology Letters. 157(2):301-306. [doi:10.1016/S0378-1097(97)00491-6]9435112
1996Dunn, MF, Encarnacion, S, Araiza, G, Vargas, MC, Davalos, A, Peralta, H, Mora, Y, Mora, J. (1996). "Pyruvate carboxylase from Rhizobium etli: Mutant characterization, nucleotide sequence, and physiological role". Journal Of Bacteriology. 178(20):5960-5970. [doi:10.1128/jb.178.20.5960-5970.1996]8830693
1995ENCARNACION, S, DUNN, M, WILLMS, K, MORA, J. (1995). "Fermentative and aerobic metabolism in Rhizobium etli". Journal Of Bacteriology. 177(11):3058-3066. [doi:10.1128/jb.177.11.3058-3066.1995]7768801