Contact
| Pablo Vinuesa (Investigador Tit. A, SNI-II, PRIDE-D) (Responsable del Posgrado en C. Biomédicas Responsible of the PhD Program in Biomedical Sciences) Centro de Ciencias Genómicas, UNAM A.P. 565-A Av. Universidad s/n, Col. Chamilpa, C.P. 62210, Cuernavaca, Morelos, Mexico map |
Tel(s). (777) 317 5867,(55) 5622 7691 Fax. (777) 317 5581 Red UNAM: 27691 Int. 117 
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About
I'm a microbiologist interested in host-microbe interactions, microbial ecology, genomics and evolution. (Biography)
Academic information and resources on this site:
- 1) A synopsis of the research projects I'm involved in, along with an introduction to the students, friends and colleagues involved in them.
- 2) A list of my publications and article downloads.
- 3) Hands-on tutorials on how to perform phylogenetic analyses using sequence data, from SSU rRNA sequence alignment and model fitting, up to ML tree searches and hypothesis testing. Many other tutorials and full-length course materials are provided as PDF files (tutoriales y cursos de bioinformática y filogenética en español), available only in Spanish at the present time.
- 4) Information on current issues in rhizobial taxonomy, systematics and genomics.
- 5) Links to bioinformatic data mining, programming and analysis tools I use frequently, including our recent primers4clades web server.
- primers4clades (primers for clades) is an easy-to-use, interactive web server for PCR primer design for cross-species amplification of novel sequences from metagenomic DNA or from uncharacterized organisms belonging to user-specified phylogenetic lineages or taxa. It implements an extended CODEHOP primer design strategy, based on both DNA and protein multiple sequence alignments of coding sequences. It evaluates a comprehensive set of thermodynamic parameters of the oligonucleotide pairs, as well as the phylogenetic information content of the theoretical amplicons, which is computed from the branch support values of maximum likelihood phylogenies estimated for each molecular marker. Phylogenetic trees displayed on screen make it easy to target the primer design for particular species groups or sequence clusters selected by the user. A detailed documentation and tutorial can be found here.
- Mirror sites: primers4clades - Mexico and primers4clades - Spain. We would appreciate your feedback!