Transcriptional Regulation of the Heat Shock Regulon in Rhizobium etli.


In all organisms, a sudden increase in temperature leads to the accumulation of partially unfolded proteins which tend to form life-threatening aggregates within cells. These non-native proteins induce the activation of the heat shock regulon. The bioinformatic analysis of the R. etli genome suggests that the heat shock regulon comprises about 30 different genes whose expression is controlled by two copies of the alternative sigma factor RpoH ( s 32 ). Preliminary results indicate that RpoH1 and RpoH2 are not functionally equivalent. To understand the different roles of both copies of RpoH, we want to identify the expression conditions of the rpoH1 and rpoH2 genes, the set of genes that are induced by RpoH1 and/or RpoH2, and the promoters recognized by these proteins.

Participants:

Biól. Alma Ruth Reyes González, Doctoral thesis.
Dr. Miguel A. Ramírez, Director.


Evolutionary Genomics Program