Regulation of conjugative transfer of the symbiotic plasmid of Rhizobium etli

In this line, we are studying the mechanisms involved in repression of a conjugative system present on the pSym of R. etli. This system, which employs one repressor gene rctA) also has interactions with another gene product (RctB) whose overproduction apparently overrides the repression imposed by RctA. Characterization of the regions relevant for transcriptional repression as well as purification and analysis of RctA and RctB are the
objectives of this line.

Participants: Edgardo Sepúlveda, Susana Brom, David Romero, Otto Geiger (external collaboration), Isabel López Lara (external collaboration), Juan Sanjuán (external collaboration).
Genome Engineering Program