Pablo Vinuesa Fleischmann

Ingeniería Genómica Investigador

Inv. Tit. B T.C. Definitivo

Semblanza

I’m a microbiologist interested in host-microbe interactions and microbial ecology, with a strong focus on comparative and evolutionary genomics. In general terms, research in my group aims to learn how niche specialization, including host adaptation, drives speciation in selected alpha- and gamma-Proteobacteria. I develop open-source software tools for microbial pan-genomics and phylogenomics, which are freely available at https://github.com/vinuesa. I’m also actively involved in teaching phylogenomics @LCG and diverse posgraduate programs @UNAM and abroad.

For more details please visit:
personal website: http://www.ccg.unam.mx/~vinuesa/
Google Scholar: http://bit.ly/2FUgqnR
ORCID: http://orcid.org/0000-0001-6119-2956
GitHub: https://github.com/vinuesa
twitter: @pvinmex

Current research
Our current work focuses on elucidating the roles and contributions of mobile genetic elements, efflux pumps, resistance genes, secretion systems and the exoproteins they secrete in the emergence of opportunistic pathogenic strains of diverse alpha- (Rhizobiales) and gamma-Proteobacteria (particularly Salmonella and Stenotrophomonas).

We use comparative and functional genomics approaches to elucidate the evolutionary processes and molecular mechanisms underlying the origin of these opportunistic pathogens and their adaptation to anthropogenized habitats. We develop open-source software for these tasks, mainly GET_HOMOLOGUES and GET_PHYLOMARKERS, which are freely available at https://github.com/vinuesa.

Current funding
Research in Vinuesa’s lab is currently (2018) funded by CONACyT and PAPIIT grants.
The Salmonella work is performed in the frame of a large CONACyT – Fronteras de la Ciencia grant (FC-2105-2-879) entitled “Descubriendo el impacto del genoma accesorio en la evolución de cepas atípicas de Salmonella Typhimurium causantes de infecciones sistémicas en México”, together with Drs. Edmundo Calva, José Luis Puente and Claudia Silva from the Institute of Biotechnology (IBT-UNAM).
The Stenotrophomonas work is funded by DGAPA-PAIIT/UNAM grant (IN206318) entitled: “Predicción genómica y análisis funcional de determinantes de resistencia y virulencia en Stenotrophomonas maltophilia, un patógeno oportunista emergente”.

Group and Publications
Please visit http://www.ccg.unam.mx/~vinuesa/group.html to meet our group members and http://www.ccg.unam.mx/~vinuesa/publications.html for an updated list of publications.

Current group members
Claudia Torres (posdoctoral researcher with a DGAPA-UNAM fellowship, September 2018-1 ->)
Claudia works on “comparative and functional genomics of Type II Secretion Systems in Stenotrophomonas”
Daniel Cazares (PhD student; PDCB-UNAM, August 2016-1 ->).
Daniel works on: “Isolation and genomic characterization of polyvalent phages from environmental matrices and Proteobacterial isolates”.
Perla Tinoco Carrillo (PhD student; PDCB-UNAM, February 2014-1 ->).
She works on: “Diversity and resistance profiles of multidrug-resistant Proteobacteria inhabiting soils of Morelos, Mexico”.
Luz Edith Ochoa Sánchez (PhD student; PDCB-UNAM, August 2013-1 ->). She works on: “Phylogenetic and phenotypic characterization of multidrug-resistant Proteobacteria recovered from Mexican rivers with contrasting contamination levels.”

More details at http://www.ccg.unam.mx/~vinuesa/group.html

Ligas

OrcID
Scopus

Teléfono: (777) 3175867
Correo: vinuesa@ccg.unam.mx

Publicaciones

AñoPublicaciónPMID
2018Sandner-Miranda, Luisa, Vinuesa, Pablo, Cravioto, Alejandro, Morales-Espinosa, Rosario (2018) The Genomic Basis of Intrinsic and Acquired Antibiotic Resistance in the Genus Serratia Frontiers in Microbiology 9(MAY):828-828 [doi:10.3389/fmicb.2018.00828]29867787
2018Vinuesa, P., Ochoa-Sánchez, L.E., Contreras-Moreira, B. (2018) GET_PHYLOMARKERS, a software package to select optimal orthologous clusters for phylogenomics and inferring pan-genome phylogenies, used for a critical geno-taxonomic revision of the genus Stenotrophomonas Frontiers in Microbiology 9(MAY):771-771 [doi:10.3389/fmicb.2018.00771]29765358
2017Ochoa-Sánchez LE, Vinuesa P (2017) Evolutionary Genetic Analysis Uncovers Multiple Species with Distinct Habitat Preferences and Antibiotic Resistance Phenotypes in the Stenotrophomonas maltophilia Complex Frontiers in Microbiology 8():1548-1548 [doi:10.3389/fmicb.2017.01548]28861062
2017Cubillas, C., Miranda-Sánchez, F., González-Sánchez, A., Elizalde, J.P., Vinuesa, P., Brom, S., García-de los Santos, A. (2017) A comprehensive phylogenetic analysis of copper transporting P-1B ATPases from bacteria of the Rhizobiales order uncovers multiplicity, diversity and novel taxonomic subtypes MICROBIOLOGYOPEN 6(4):e00452- [doi:10.1002/mbo3.452]28217917
2017Santamaría RI, Bustos P, Pérez-Carrascal OM, Miranda-Sánchez F, Vinuesa P, Martínez-Flores I, Juárez S, Lozano L, Martínez-Romero E, Cevallos MÁ, Romero D, Dávila G, Ormeño-Orrillo E, González V (2017) Complete Genome Sequences of Eight Rhizobium Symbionts Associated with Common Bean (Phaseolus vulgaris). Genome Announcements 5(30):- [doi:10.1128/genomeA.00645-17]28751391
2017Martínez-Flores, W.A., Palma-García, J.M., Caballero-Ortega, H., Del Viento-Camacho, A., López-Escamilla, E., Martínez-Hernández, F., Vinuesa, P., Correa, D., Maravilla, P. (2017) Genotyping Toxoplasma Gondii with the B1 Gene in Naturally Infected Sheep from an Endemic Region in the Pacific Coast of Mexico VECTOR-BORNE AND ZOONOTIC DISEASES 17(7):495-502 [doi:10.1089/vbz.2016.2085]28530509
2017Bustos P, Santamaría RI, Pérez-Carrascal OM, Acosta JL, Lozano L, Juárez S, Martínez-Flores I, Martínez-Romero E, Cevallos MÁ, Romero D, Dávila G, Vinuesa P, Miranda F, Ormeño E, González V (2017) Complete Genome Sequences of Three Rhizobium gallicum Symbionts Associated with Common Bean (Phaseolus vulgaris). Genome Announcements 5(11):- [doi:10.1128/genomeA.00030-17]28302777
2017Contreras-Moreira B, Cantalapiedra CP, García-Pereira MJ, Gordon SP, Vogel JP, Igartua E, Casas AM, Vinuesa P (2017) Analysis of Plant Pan-Genomes and Transcriptomes with GET_HOMOLOGUES-EST, a Clustering Solution for Sequences of the Same Species Frontiers In Plant Science 8():184-184 [doi:10.3389/fpls.2017.00184]28261241
2016Miranda-Sanchez, Fabiola, Rivera, Javier, Vinuesa, Pablo (2016) Diversity patterns of Rhizobiaceae communities inhabiting soils, root surfaces and nodules reveal a strong selection of rhizobial partners by legumes Environmental Microbiology 18(8):2375-2391 [doi:10.1111/1462-2920.13061]26395550
2016Perez Carrascal, Olga M., VanInsberghe, David, Juarez, Soledad, Polz, Martin F., Vinuesa, Pablo, Gonzalez, Victor (2016) Population genomics of the symbiotic plasmids of sympatric nitrogen-fixing Rhizobium species associated with Phaseolus vulgaris Environmental Microbiology 18(8):2660-2676 [doi:10.1111/1462-2920.13415]27312778
2016Centeno-Leija, S., Vinuesa, P., Karol Rodr�guez-Pe�a,, Trenado-Uribe, M., Yair C�rdenas-Conejo,, Serrano-Posada, H., Romina Rodr�guez-Sanoja,, S�ncheza, S. (2016) Draft genome Sequence of an endophytic actinoplanes species, encoding uncommon trans-Acyltransferase polyketide synthases Genome Announcements 4(2):e00164-16- [doi:10.1128/genomeA.00164-16]27013046
2016Sandner-Miranda, L., Vinuesa, P., Sober�n-Ch�vez, G., Morales-Espinosa, R. (2016) Complete genome sequence of Serratia marcescens SmUNAM836, a nonpigmented multidrug-resistant strain isolated from a Mexican patient with obstructive pulmonary disease Genome Announcements 4(1):e01417-15- [doi:10.1128/genomeA.01417-15]26798087
2016Silva, C., Calva, E., Puente, J.L., Zaidi, M.B., Vinuesa, P. (2016) Complete genome sequence of Salmonella enterica serovar Typhimurium strain YU15 (sequence type 19) harboring the Salmonella genomic island 1 and virulence plasmid pSTV Genome Announcements 4(2):e00252-16- [doi:10.1128/genomeA.00252-16]27081132
2016Vinuesa, P., Puente, J.L., Calva, E., Zaidi, M.B., Silva, C. (2016) Complete genome sequence of Salmonella enterica serovar Typhimurium strain SO3 (sequence type 302) isolated from a baby with meningitis in Mexico Genome Announcements 4(2):e00285-16- [doi:10.1128/genomeA.00285-16]27103717
2016Silva, C., Calva, E., Puente, J.L., Zaidi, M.B., Vinuesa, P. (2016) Complete genome sequence of Salmonella enterica serovar Typhimurium strain SO2 (sequence type 302) isolated from an asymptomatic child in Mexico Genome Announcements 4(2):e00253-16- [doi:10.1128/genomeA.00253-16]27081133
2015Riquelme, Cristina, Camara, Sandra, Enes Dapkevicius, Maria de Lurdes N., Vinuesa, Pablo, Gomes da Silva, Celia Costa, Xavier Malcata, F., Rego, Oldemiro A. (2015) Characterization of the bacterial biodiversity in Pico cheese (an artisanal Azorean food) International Journal Of Food Microbiology 192():86-94 [doi:10.1016/j.ijfoodmicro.2014.09.031]25440551
2015Vinuesa, P., Contreras-Moreira, B. (2015) Pangenomic Analysis of the Rhizobiales Using the GET_HOMOLOGUES Software Package Biological Nitrogen Fixation 1-2():271-280 [doi:10.1002/9781119053095.ch27]
2015Vinuesa, P., Contreras-Moreira, B. (2015) Robust Identification of Orthologues and Paralogues for Microbial Pan-Genomics Using GET_HOMOLOGUES: A Case Study of pIncA/C Plasmids Bacterial Pangenomics: Methods And Protocols 1231():203-232 [doi:10.1007/978-1-4939-1720-4_14]25343868
2015Vinuesa, P., Ochoa-S�nchez, L.E. (2015) Complete genome sequencing of Stenotrophomonas acidaminiphila ZAC14D2_NAIMI4_2, a multidrug-resistant strain isolated from sediments of a polluted river in Mexico, uncovers new antibiotic resistance genes and a novel class-II lasso peptide biosynthesis gene cluster Genome Announcements 3(6):e0143315- [doi:10.1128/genomeA.01433-15]26659678
2014Cubillas, Ciro, Vinuesa, Pablo, Tabche, Maria Luisa, Davalos, Araceli, Vazquez, Alejandra, Hernandez-Lucas, Ismael, Romero, David, Garcia-de los Santos, Alejandro (2014) The cation diffusion facilitator protein EmfA of Rhizobium etli belongs to a novel subfamily of Mn2+/Fe2+ transporters conserved in alpha-proteobacteria Metallomics 6(10):1808-1815 [doi:10.1039/c4mt00135d]25054342
2014Chang, F.M.J., Coyne, H.J., Cubillas Ramírez, Ciro Alberto, Vinuesa Fleischmann, Pablo, Fang, X.Y., Ma, Z., Ma, D.J., Helmann, J.D., García de los Santos, Alejandro, Wang, Y.X., Dann, C.E., Giedroc, D.P. (2014) Cu(I)-mediated Allosteric Switching in a Copper-sensing Operon Repressor (CsoR) JOURNAL OF BIOLOGICAL CHEMISTRY 289(27):19204-19217 [doi:10.1074/jbc.M114.556704]24831014
2013Cubillas Ramírez, Ciro Alberto, Vinuesa Fleischmann, Pablo, Tabche, M.L., García de los Santos, Alejandro (2013) Phylogenomic analysis of Cation Diffusion Facilitator proteins uncovers Ni2+/Co2+ transporters Metallomics 5(12):1634-1643 [doi:10.1039/c3mt00204g]24077251
2013Contreras-Moreira, B., Vinuesa Fleischmann, Pablo (2013) GET_HOMOLOGUES, a Versatile Software Package for Scalable and Robust Microbial Pangenome Analysis Applied And Environmental Microbiology 79(24):7696-7701 [doi:10.1128/AEM.02411-13]24096415
2013Estrada De Los Santos, Paulina, Vinuesa Fleischmann, Pablo, Martínez Aguilar, Lourdes, Hirsch, A.M., Caballero Mellado, José de Jesús (2013) Phylogenetic Analysis of Burkholderia Species by multilocus Sequence Analysis Current Microbiology 67(1):51-60 [doi:10.1007/s00284-013-0330-9]23404651
2012Escalera-Zamudio, M., Cobian-Guemes, G., Soto-del Río, M.D., Isa, P., Sánchez-Betancourt, I., Parissi-Crivelli, A., Martinez-Cazares, M.T., Romero, P., Velázquez-Salinas, L., Huerta-Lozano, B., Nelson, M., Montero, H., Vinuesa Fleischmann, Pablo, López, S., Arias, C.F. (2012) Characterization of an influenza A virus in Mexican swine that is related to the A/H1N1/2009 pandemic clade Virology 433(1):176-182 [doi:10.1016/j.virol.2012.08.003]22921315
2012Castillo-Rodal, A.I., Mazari-Hiriart, M., Lloret-Sánchez, L.T., Sachman Ruiz, Bernardo, Vinuesa Fleischmann, Pablo, López-Vidal, Y (2012) Potentially pathogenic nontuberculous mycobacteria found in aquatic systems. Analysis from a reclaimed water and water distribution system in Mexico City European Journal Of Clinical Microbiology and Infectious Diseases 31(5):683-694 [doi:10.1007/s10096-011-1359-y]21805195
2011Acosta, Jose L., Eguiarte, L.E., Santamaria Gutiérrez, Rosa Isela, Bustos Arcos, Virginia Patricia, Vinuesa P, Martínez Romero, María Esperanza, Dávila Ramos, José Guillermo, González Zúñiga, Víctor Manuel (2011) Genomic lineages of Rhizobium etli revealed by the extent of nucleotide polymorphisms and low recombination Bmc Evolutionary Biology 11():305-305 [doi:10.1186/1471-2148-11-305]22004448
2011Cervantes de la Luz, Laura, Bustos Arcos, Virginia Patricia, Girard Cuesy, María de Lourdes, Santamaria Gutiérrez, Rosa Isela, Dávila Ramos, José Guillermo, Vinuesa Fleischmann, Pablo, Romero Camarena, David René, Brom Klanner, Susana (2011) The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain Bmc Microbiology 11(1):1-11 [doi:10.1186/1471-2180-11-149]21702991
2011Sachman-Ruiz, B., Contreras-Moreira, B., Zozaya, E., Martínez-Garza, C., Vinuesa, P. (2011) Primers4clades: A Web Server to Design Lineage-Specific PCR Primers for Gene-Targeted Metagenomics Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches 1():441-452 [doi:10.1002/9781118010518.ch51]
2011Silva-Sanchez, J., Garza-Ramos, J.U., Reyna-Flores, F., Sánchez-Pérez, A, Rojas-Moreno, T., Andrade-Almaraz, V., Pastrana, J., Castro-Romero, J.I., Vinuesa Fleischmann, Pablo, Barrios, H., Cervantesde, C (2011) Extended-spectrum beta-Lactamase-Producing Enterobacteriaceae Causing Nosocomial Infections in Mexico. A Retrospective and Multicenter Study Archives Of Medical Research 42(2):156-162 [doi:10.1016/j.arcmcd.2011.02.004]21565630
2010Vinuesa Fleischmann, Pablo (2010) multilocus Sequence Analysis and Bacterial Species Phylogeny Estimation Molecular Phylogeny of Microorganisms ():41-64
2009Cummings, Stephen P., Gyaneshwar, Prasad, Vinuesa, Pablo, Farruggia, Frank T., Andrews, Mitchell, Humphry, David, Elliott, Geoffrey N., Nelson, Andrew, Orr, Caroline, Pettitt, Deborah, Shah, Gopit R., Santos, Scott R., Krishnan, Hari B., Odee, David, Moreira, Fatima M. S., Sprent, Janet I., Young, J. Peter W., James, Euan K. (2009) Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other Rhizobia Environmental Microbiology 11(10):2510-2525 [doi:10.1111/j.1462-2920.2009.01975.x]19555380
2009Contreras-Moreira, Bruno, Sachman-Ruiz, Bernardo, Figueroa-Palacios, Irais, Vinuesa, Pablo (2009) primers4clades: a web server that uses phylogenetic trees to design lineage-specific PCR primers for metagenomic and diversity studies Nucleic Acids Research 37(Web Server issue):95-100 [doi:10.1093/nar/gkp377]19465390
2008Renteria, Miguel E., Gandhi, Neha S., Vinuesa, Pablo, Helmerhorst, Erik, Mancera, Ricardo L. (2008) A Comparative Structural Bioinformatics Analysis of the Insulin Receptor Family Ectodomain Based on Phylogenetic Information Plos One 3(11):e3667- [doi:10.1371/journal.pone.0003667]18989367
2008Vinuesa, Pablo, Rojas-Jimenez, Keilor, Contreras-Moreira, Bruno, Mahna, Suresh K., Prasad, Braj Nandan, Moe, Hla, Selvaraju, Suresh Babu, Thierfelder, Heidemarie, Werner, Dietrich (2008) multilocus Sequence Analysis for Assessment of the Biogeography and Evolutionary Genetics of Four Bradyrhizobium Species That Nodulate Soybeans on the Asiatic Continent Applied And Environmental Microbiology 74(22):6987-6996 [doi:10.1128/AEM.00875-08]18791003
2008Cerritos, Rene, Vinuesa, Pablo, Eguiarte, Luis E., Herrera-Estrella, Luis, Alcaraz-Peraza, Luis D., Arvizu-Gomez, Jackeline L., Olmedo, Gabriela, Ramirez, Enrique, Siefert, Janet L., Souza, Valeria (2008) Bacillus coahuilensis sp nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Cienegas Valley in Coahuila, Mexico International Journal Of Systematic And Evolutionary Microbiology 58(4):919-923 [doi:10.1099/ijs.0.64959-0]18398195
2007Sohlenkamp, Christian, Galindo-Lagunas, Kanaan A., Guan, Ziqiang, Vinuesa, Pablo, Robinson, Sally, Thomas-Oates, Jane, Raetz, Christian R. H., Geiger, Otto (2007) The lipid lysyl-phosphatidylglycerol is present in membranes of Rhizobium tropici CIAT899 and confers increased resistance to polymyxin B under acidic growth conditions Molecular Plant-Microbe Interactions 20(11):1421-1430 [doi:10.1094/MPMI-20-11-1421]17977153
2007Poggio, Sebastian, Abreu-Goodger, Cei, Fabela, Salvador, Osorio, Aurora, Dreyfus, Georges, Vinuesa, Pablo, Camarena, Laura (2007) A complete set of flagellar genes acquired by horizontal transfer coexists with the endogenous flagellar system in Rhodobacter sphaeroides Journal Of Bacteriology 189(8):3208-3216 [doi:10.1128/JB.01681-06]17293429
2007Silva Romero, Claudia Verónica, Vinuesa Fleischmann, Pablo (2007) Ecología evolutiva de bacterias y el concepto de especie: el caso de los rizobios Ecología Molecular ():351-392
2006Albino, U., Saridakis, D. P., Ferreira, M. C., Hungria, M., Vinuesa, P., Andrade, G. (2006) High diversity of diazotrophic bacteria associated with the carnivorous plant Drosera villosa var. villosa growing in oligotrophic habitats in Brazil PLANT AND SOIL 287(1-2):199-207 [doi:10.1007/s11104-006-9066-7]
2006Ormeño Orrillo, Ernesto Aldo, Vinuesa Fleischmann, Pablo, Zuniga-Davila, D.Z., Martínez Romero, María Esperanza (2006) Molecular diversity of native bradyrhizobia isolated from Lima bean (Phaseolus lunatus L.) in Peru Systematic And Applied Microbiology 29(3):253-262 [doi:10.1016/j.syapm.2005.09.002]16564961
2006Silva Romero, Claudia Verónica, Vinuesa Fleischmann, Pablo, Martínez Romero, María Esperanza (2006) Species delineation and biogeography of Rhizobia: Two case studies Proceedings of the 12th International Congress on Molecular Plant-Microbe Interactions 5():319-324
2005Rojas-Jimenez, K., Sohlenkamp, Christian, Geiger, Otto, Martínez Romero, María Esperanza, Werner, D., Vinuesa Fleischmann, Pablo (2005) A CIC chloride channel homolog and ornithine-containing membrane lipids of Rhizobium tropici CIAT899 are involved in symbiotic efficiency and acid tolerance Molecular Plant-Microbe Interactions 18(11):1175-1185 [doi:10.1094/MPMI-18-1175]16353552
2005Silva Romero, Claudia Verónica, Vinuesa Fleischmann, Pablo, Eguiarte, L.E., Souza, V., Martínez Romero, María Esperanza (2005) Evolutionary genetics and biogeographic structure of Rhizobium gallicum sensu lato, a widely distributed bacterial symbiont of diverse legumes MOLECULAR ECOLOGY 14(13):4033-4050 [doi:10.1111/j.1365-294X.2005.02721.x]16262857
2005Vinuesa Fleischmann, Pablo, Silva Romero, Claudia Verónica, Lorite, M.J., Izaguirre-Mayoral, M.L., Bedmar, E.J., Martínez Romero, María Esperanza (2005) Molecular systematics of Rhizobia based on maximum likelihood and Bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania microsymbionts from Venezuelan wetlands Systematic And Applied Microbiology 28(8):702-716 [doi:10.1016/j.syapm.2005.05.007]16261860
2005Vinuesa Fleischmann, Pablo, Leon-Barrios, M., Silva Romero, Claudia Verónica, Willems, A., Jarabo-Lorenzo, A., Perez-Galdona, R., Werner, D., Martínez Romero, María Esperanza (2005) Bradyrhizobium canariense sp nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae : Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta International Journal Of Systematic And Evolutionary Microbiology 55(2):569-575 [doi:10.1099/ijs.0.63292-0]15774626
2005Vinuesa Fleischmann, Pablo, Silva Romero, Claudia Verónica, Werner, D., Martínez Romero, María Esperanza (2005) Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineation Molecular Phylogenetics And Evolution 34(1):29-54 [doi:10.1016/j.ympev.2004.08.020]15579380
2005Shamseldin, AAY, Vinuesa, P, Thierfelder, H, Werner, D (2005) Rhizobium etli and Rhizobium gallicum nodulate Phaseolus vulgaris in Egyptian soils and display cultivar-dependent symbiotic efficiency Symbiosis 38(2):145-161
2004Medrano Soto, Luis Arturo, Moreno Hagelsieb, Gabriel, Vinuesa Fleischmann, Pablo, Christen, J.A., Collado Vides, Pedro Julio (2004) Successful lateral transfer requires codon usage compatibility between foreign genes and recipient genomes MOLECULAR BIOLOGY AND EVOLUTION 21(10):1884-1894 [doi:10.1093/molbev/msh202]15240837
2003Vinuesa Fleischmann, Pablo, Neumann-Silkow, F., Pacios-Bras, C., Spaink, H.P., Martínez Romero, María Esperanza, Werner, D. (2003) Genetic analysis of a pH-regulated operon from Rhizobium tropici CIAT899 involved in acid tolerance and nodulation competitiveness Molecular Plant-Microbe Interactions 16(2):159-168 [doi:10.1094/MPMI.2003.16.2.159]12575750
2003Silva, C, Vinuesa, P, Eguiarte, LE, Martinez-Romero, E, Souza, V (2003) Rhizobium etli and Rhizobium gallicum nodulate common bean (Phaseolus vulgaris) in a traditionally managed milpa plot in Mexico: Population genetics and biogeographic implications Applied And Environmental Microbiology 69(2):884-893 [doi:10.1128/AEM.69.2.884-893.2003]12571008
2000Jarabo-Lorenzo, A, Velazquez, E, Perez-Galdona, R, Vega-Hernandez, MC, Martinez-Molina, E, Mateos, PF, Vinuesa, P, Martinez-Romero, E, Leon-Barrios, M (2000) Restriction fragment length polymorphism analysis of 16S rDNA and low molecular weight RNA profiling of rhizobial isolates from shrubby legumes endemic to the Canary islands Systematic And Applied Microbiology 23(3):418-425 [doi:10.1016/S0723-2020(00)80073-9]11108022