BACKGROUND: Among the
numerous in vitro methods for studying the antimicrobial activity of
plant drugs, bioautography has found widespread applications, especially
for the detection of new compounds in complex plant extracts.
paper describes the results obtained during the application of the
bioautographic method to detect antimicrobial compounds in a
chloroformic extract of leaves and stems of Bocconia arborea, a plant
used profusely in traditional medicine for the treatment of diverse
RESULTS AND CONCLUSIONS: The
methods allows for the detection of spots of growth inhibition of
cultures directly in the extract thin layer chromatographic plate
previously dispersed with a broth culture containing the microorganisms.
The procedure also allowed for the detection of the presence of several
products in the B. arborea extract with considerable activity against
five different microorganisms. Additionally, the method allowed the
determination that the antimicrobial activity is due to compounds of
probable alkaloid origin.