A new specific method to detect cyanide in body fluids, especially whole blood, by fluorimetry.


By jpeza - Posted on 09 Noviembre 2010

Fecha Publicación: 
1 Ene 1998
Datos del paper
Autor Principal: 
Felscher D
Volumen: 
22
Issue: 
(5)
Página Inicial: 
363
Página Final: 
6.
Abstract: 

This study shows a simple, rapid, and specific method for the
quantitative determination of cyanide ion in body fluids, especially
blood, by fluorimetry. It is based upon the transformation of cyanide
ion into hydrocyanic acid, which then reacts with
2,3-naphthalenedialdehyde and taurine in a self-contained system. The
1-cyano-2-benzoisoindole derivate thus formed is suitable for
fluorimetric measurement (lambdaEX = 418 nm; lambdaEM = 460 nm). The
fluorescence intensity can be determined by spectrophotometry or by
high-performance liquid chromatography (HPLC) with fluorescence
detection. The detection limit is 0.002 microg/mL. Linearity was
excellent from 0.002 to 1 microg/mL for spectrophotometry and from 0.002
to 5 microg/mL for HPLC with fluorescence detection. The coefficient of
variation for repeatability was 8% or less. Thiocyanate and sulfide did
not interfere, even at high concentrations (200 microg/mL). The method
was applicable to whole blood, so it should be suitable for both
clinical and forensic purposes.

Dirección del Autor: 

Institut für Rechtsmedizin des Universitätsklinikums Carl Gustav Carus der Technischen Universität Dresden, Germany.

Keywords: 
body fluids ; fluorimetry
Coautores: 

Wulfmeyer M.

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