Dipeptidyl peptidase IV in the immune system. Effects of specific enzyme inhibitors on activity of dipeptidyl peptidase IV and p


By jpeza - Posted on 09 Noviembre 2010

Datos del paper
Autor Principal: 
Schön E
Volumen: 
372
Issue: 
(5)
Página Inicial: 
305
Página Final: 
11
Abstract: 

Dipeptidyl peptidase IV (DP IV) is a membrane peptidase playing a
significant role in the process of activation and proliferation of human
thymus-derived lymphocytes. This conclusion is drawn from (1) the
induction of this enzyme on mitogen-activated T lymphocytes (cf. Schön,
E. & Ansorge, S. (1990) Biol. Chem. Hoppe-Seyler 371, 699-705) and
(2) the impairment of different functions of activated T cells in the
presence of specific inhibitors and antibodies against DP IV (Schön, E.
& al. (1987) Eur. J. Immunol 17, 1821-1826). This paper is aimed at
testing new active site-specific peptide inhibitors for their efficiency
as inhibitors of lymphocyte DP IV and DNA synthesis of
mitogen-stimulated lymphocytes. These inhibitors comprise (i)
diacylhydroxylamine derivatives of Xaa-Pro or Xaa-Ala peptides, (ii)
different oligopeptides with N-terminal Xaa-Pro-sequences, and (iii)
amino-acid amides of the pyrrolidide and the thiazolidide type. The
thiazolidides of epsilon-(4-nitrobenzyloxycarbonyl)-L-lysine and of
L-isoleucine as well as Ala-Pro-nitrobenzoylhydroxylamine are the most
effective inhibitors in both test systems, yielding half-maximal
inhibitory concentrations in the micromolar range. Cell viability was
not impaired in this effective concentration range. Other inhibitors of
DP IV are one to two orders of magnitude less efficient in the
suppression of lymphocyte proliferation.

Dirección del Autor: 

Medizinische Akademie Magdeburg, Klinik für Innere Medizin, Forschungsabteilung Experimentelle Immunologie

Keywords: 
Dipeptidyl peptidase IV ; immune system
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