Characterization of a polysaccharide antigen from Bacteroides gingivalis


By jpeza - Posted on 09 Noviembre 2010

Fecha Publicación: 
1 Ene 1989
Nombre de Revista: 
Datos del paper
Autor Principal: 
RE Schifferle
Volumen: 
143
Issue: 
9
Página Inicial: 
3035
Página Final: 
3042
Abstract: 

A polysaccharide Ag (PS) was isolated from the phenol-water extract of
Bacteroides gingivalis strain A7A1-28 and separated from LPS by Sephacryl
S-400 HR chromatography. The PS was composed of glucose, glucosamine,
galactosamine, and galactosaminuronic acid, while the LPS contained
rhamnose, mannose, galactose, glucose, glucosamine, galactosamine,
phosphate, and lipid, but not galactosaminuronic acid. The PS and LPS were
immunochemically distinct by immunoelectrophoresis in agarose with
homologous rabbit antiserum. The phenol-water extract from strain A7A1-28
was immunoreactive by immunoelectrophoresis against antisera to three
additional strains of B. gingivalis, however, the PS was only reactive with
homologous serum. Immunochemical characterization of decarboxylated and
deacetylated PS derivatives suggest that the acetylation of the amino
sugars, but not the presence of the carboxylate residue on
galactosaminuronic acid contributes to major immunodeterminant expression

Dirección del Autor: 

Department of Oral Biology, School of Dental Medicine, SUNY, Buffalo 14214.

Keywords: 
polysaccharide Ag ; Bacteroides gingivalis
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