Coaggregation betweenandamong human intestinal and oral bacteria


By jpeza - Posted on 21 Octubre 2009

Fecha Publicación: 
12 Jun 2008
Nombre de Revista: 
Datos del paper
Autor Principal: 
Ruth G. Ledder
Volumen: 
66
Issue: 
3
Página Inicial: 
630
Página Final: 
636
Abstract: 

Coaggregation is believed to facilitate the integration of new bacterial species into
polymicrobial communities. The aim of this study was to investigate coaggregation
between and among human oral and enteric bacteria. Stationary phase cultures of
10 oral and 10 enteric species, chosen on the basis of numerical and ecological
significance in their respective environments together with their ease of cultivation,
were tested using a quantitative spectrophotometric coaggregation assay in all
possible pairwise combinations to provide quantitative coaggregation scores.While
40% of possible partnerships coaggregated strongly for oral strains, strong interactions
between oral and gut strains were considerably less common (4% incidence).
Coaggregation scores were also weak between members of the intestinal microbiota
(7% incidence), apart from Bacteroides fragilis with Clostridium perfringens, and
Bifidobacterium adolescentis with C. perfringens. Oral and intestinal bacteria did not
strongly interact, apart from B. adolescentis with Fusobacterium nucleatum, Actinomyces
naeslundii with C. perfringens and F. nucleatum with Lactobacillus paracasei.
Heating and sugar-addition experiments indicated that similar to oral microorganisms,
interactions within intestinal bacteria and between intestinal and oral strains
were mediated by lectin–carbohydrate interactions

Dirección del Autor: 

Sandra Macfarlane, Gut
Group, University of Dundee, Level 6,
Ninewells Hospital Medical School, Dundee
DD1 9SY, UK. Tel.: 144 0 1382 632 535; fax:
144 0 1382 633 952; e-mail:
s.macfarlane@dundee.ac.uk

Keywords: 
coaggregation; oral bacteria; intestinal bacteria; lectins.
Coautores: 

Andrea S. Timperley, Melissa K. Friswell, Sandra Macfarlane , Andrew J. McBain