The presence ofa1-aminocyclopropane-1-carboxylate (ACC) deaminase deletionmutationalters the physiologyof the endophytic plant g

By jpeza - Posted on 21 Octubre 2009

Fecha Publicación: 
7 May 2009
Nombre de Revista: 
Datos del paper
Autor Principal: 
Yili Sun
Página Inicial: 
Página Final: 

The structural gene for 1-aminocyclopropane-1-carboxylate (ACC) deaminase
(acdS) from the endophytic plant growth-promoting bacterium Burkholderia
phytofirmans PsJN was isolated and used to construct a mutant strain B. phytofirmans
YS2 (B. phytofirmans PsJN/DacdS), in which an internal segment of the acdS
gene was deleted. The mutant YS2 lost ACC deaminase activity as well as the ability
to promote the elongation of the roots of canola seedlings. Concomitant with the
creation of this deletion mutant, a number of physiological changes were observed
in the bacterium, including an increase in indole acetic acid synthesis, a decrease in
the production of siderophores and an increase in the cellular level of the
stationary-phase s factor, RpoS. Introduction of the wild-type acdS gene into the
mutant YS2 to construct strain B. phytofirmans YS3 (B. phytofirmans YS2/pRKAcdS)
restored both ACC deaminase activity and plant growth-promotion activity
in strain YS3. However, the complemented mutant still showed the abovementioned
physiological changes.

Dirección del Autor: 

Department of Biology, University of
Waterloo, 200 University Avenue West,
Waterloo, ON, Canada N2L 3G1. Tel.: 11 519
888 4567, ext. 32058; fax: 11 519 746 0614;

ACC deaminase; endophyte; IAA; plant growth promotion; RpoS; siderophore

Zhenyu Cheng ;  Bernard R. Glick