Recent progress in living cell imaging of plant cytoskeleton and vacuole using fluorescent-protein transgenic lines and three-di


By jpeza - Posted on 14 Octubre 2009

Fecha Publicación: 
24 Abr 2007
Nombre de Revista: 
Datos del paper
Autor Principal: 
A. Yoneda
Volumen: 
230
Página Inicial: 
129
Página Final: 
139
Abstract: 

In higher-plant cells, microtubules, actin microfilaments,
and vacuoles play important roles in a variety of cellular events, including
cell division, morphogenesis, and cell differentiation. These intracellular
structures undergo dynamic changes in their shapes and functions
during cell division and differentiation, and to analyse these sequential
structural changes, the vital labelling technique, using the green-fluorescent
protein or other fluorescent proteins, has commonly been used to
follow the localisation and translocation of specific proteins. To visualise
microtubules, actin filaments, and vacuoles, several strategies are available
for selecting the appropriate fluorescent-protein fusion partner: microtubule-
binding proteins, tubulin, and plus-end-tracking proteins are
most suitable for microtubule labelling; the actin binding domain of
mouse talin and plant fimbrin for actin microfilament visualisation; and
the tonoplast-intrinsic proteins and syntaxin-related proteins for vacuolar
imaging. In addition, three-dimensional reconstruction methods are indispensable
for localising the widely distributed organelles within the
cell. The maximum intensity projection method is suitable for cytoskeletal
structures, while contour-based surface modelling possesses many advantages
for vacuolar membranes. In this article, we summarise the recent
progress in living cell imaging of the plant cytoskeleton and vacuoles using
various fusions with green-fluorescent proteins and three-dimensional
imaging techniques.

Dirección del Autor: 

Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba

Keywords: 
Actin microfilament; Microtubule; Vacuole; Three-dimensional imaging; Green-fluorescent protein.
Coautores: 

N. Kutsuna, T. Higaki, Y. Oda, T. Sano, and S. Hasezawa*

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