The lipid composition of the unicellular green alga Chlamydomonas reinhardtii and the diatom Cyclotella......


By jpeza - Posted on 09 Julio 2009

Fecha Publicación: 
1 Ene 2007
Datos del paper
Autor Principal: 
Astrid Vieler
Volumen: 
150
Página Inicial: 
143
Página Final: 
155
Abstract: 

The lipid composition of algae is crucial for numerous structural and physiological aspects, e.g. the integrity of the photosynthetic

complexes and the functionality of membrane-embedded processes as the photosynthetic electron transport in thylakoids or the

mitochondrial respiration. In this paper the lipid composition of the organic extracts of the green alga Chlamydomonas reinhardtii

and the diatom Cyclotella meneghiniana are compared by using matrix-assisted laser desorption and ionization time-of-flight

(MALDI-TOF) mass spectrometry (MS) in combination with thin-layer chromatography (TLC).

The combined methods enable quantitative evaluation of the individual lipid classes as well as the determination of the relative

acyl compositions. It will be shown that both algae differ in (a) the lipid classes, (b) the relative contribution of the individual

lipid classes and (c) the acyl compositions. Differences in the acyl composition concern particularly the mono- and digalactosyl

diacylglycerols. Glycerol-trimethylhomoserine and phosphatidylethanolamine are exclusively detected in the C. reinhardtii

extracts, whereas phosphatidylcholine is a characteristic lipid of C. meneghiniana. Furthermore, the proportion of the acidic lipids sulfoquinovosyl-diacylglycerol and phosphatidylglycerol is significantly higher in the diatom than in C. reinhardtii.

© 2007 Elsevier Ireland Ltd. All rights reserved.

Dirección del Autor: 

University of Leipzig, Faculty of Biosciences, Pharmacy and Psychology, Institute of Biology I,

Johannisallee 21-23, D-04103 Leipzig, Germany

b Faculty of Medicine, Institute of Medical Physics and Biophysics, H¨artelstr. 16-18, D-04107 Leipzig, Germany

Keywords: 
Algae; Lipids; MALDI-TOF MS; TLC
Coautores: 

Christian Wilhelm b, Reimund Goss a,

Rosmarie S¨uß b, J¨urgen Schiller b,

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