Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination


By jpeza - Posted on 09 Julio 2009

Fecha Publicación: 
1 Ene 2009
Datos del paper
Autor Principal: 
Fabricio Cassan
Volumen: 
45
Página Inicial: 
28
Página Final: 
35
Abstract: 

Inoculants are biological formulations that combine a stable microorganism population

and various types of compounds produced and released during fermentation, such as

phytohormones and plant growth regulators. Azospirillum brasilense strain Az39 and

Brayrhizobium japonicum strain E109 were previously shown to produce indole 3-acetic acid

(IAA), gibberellic acid (GA3) and zeatin (Z). We tested the hypothesis that such compoundsare responsible for early growth promotion in inoculated corn (

Zea mays L.) and soybean(Glycine max L.) seedlings. Seeds were inoculated with Az39, E109, or both, and kept in

a chamber at 20–30 C under a controlled photoperiod to evaluate seed germination. To

evaluate root and shoot length and dry weight, and number of nodules and percentage of

nodulated seedlings, in soybean, seedlings were kept in a growth chamber for 14 days

under similar photoperiod and temperature conditions. Az39 and E109, singly or in

combination, showed the capacity to promote seed germination, nodule formation, and

early development of corn and soybean seedlings. Both strains were able to excrete IAA,

GA3 and Z into the culture medium, at a concentration sufficient to produce morphological

and physiological changes in young seed tissues.

Dirección del Autor: 

aLaboratorio de Fisiologı´a Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Fı´sico-Quı´micas y

Naturales Universidad Nacional de Rı´o Cuarto, CP 5800. Rı´o Cuarto, Co´rdoba, Argentina

bDepartamento de Investigacio´n y Desarrollo Ba´sico. Nitragin Argentina SA., Pilar, Buenos Aires, Argentina

Keywords: 
Azospirillum
Bradyrhizobium ; Phytohormone ; Inoculant ; PGPR ; Plant growth promotion
Coautores: 

Diego Perriga, Verónica Sgroya, Oscar Masciarelli

Claudio Penna, Virginia Luna

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