Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli


By jpeza - Posted on 29 Junio 2009

Fecha Publicación: 
1 Ene 2008
Nombre de Revista: 
Datos del paper
Autor Principal: 
Yuzhen Zhou
Volumen: 
35
Issue: 
4
Página Inicial: 
711
Página Final: 
718
Abstract: 

The control of gene expression by enzymes

provides a direct pathway for cells to respond to fluctuations

in metabolites and nutrients. One example is the

proline utilization A (PutA) protein from Escherichia coli.

PutA is a membrane-associated enzyme that catalyzes the

oxidation of L-proline to glutamate using a flavin containing

proline dehydrogenase domain and a NAD+ dependent

D1-pyrroline-5-carboxylate dehydrogenase domain. Insome Gram-negative bacteria such as E. coli, PutA is also

endowed with a ribbon–helix–helix DNA-binding domain

and acts as a transcriptional repressor of the proline utilization

genes. PutA switches between transcriptional

repressor and enzymatic functions in response to proline

availability. Molecular insights into the redox-based

mechanism of PutA functional switching from recent

studies are reviewed. In addition, new results from cellbased

transcription assays are presented which correlate

PutA membrane localization with put gene expression

levels. General membrane localization of PutA, however, is

not sufficient to activate the put genes.

Dirección del Autor: 

Department of Biochemistry, University of Nebraska-Lincoln,

N258 Beadle Center, 19th and Vine Street, Lincoln,

NE 68588, USA

Keywords: 
PutA ; Transcriptional regulation ;Membrane-binding  Proline utilization  DNA-binding
Coautores: 

 Weidong Zhu  Padmanetra S. Bellur

Dustin Rewinkel  Donald F. Becker

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