Part 4:Evidence that lethal toxin neutralizing peptide LT-1 o binds to proteins and an exploration


By jpeza - Posted on 26 Junio 2009

Fecha Publicación: 
1 Ene 2008
Nombre de Revista: 
Datos del paper
Autor Principal: 
BINIE VER LIPPS
Volumen: 
27
Issue: 
2
Página Inicial: 
125
Página Final: 
138
Abstract: 

Natwral lethal toxin neutralizing factor (N-LTNF), rmoiecular wcight 63.0 kDa,

7uas isolated from opossum serum. Afer trypsin digation, the active domain

of N-LTNF was isolated and sequenced. A synthetic peptide cor~sisting of 10

amino acids frwm the N-LXVF was designated as LTIO. N-LTNF and LT

10 inhibited the iethality of toxins frwm an%mals, plants, and bacteria when

tested in mice by a non,-kmmunological ~mechanism. Howmer, the antibodies

against N-LTW and LTIO reacted zmmunolog2'cally with toxins only and not

with nontoxic substances. Anti-LTW and anti-LTlO detected toxins in an

ELISA assay that were not detected by a mouse toxicity test, includir~gc holera

toxin and digoxin. EUrthermore, anti-N-LTNF and anti-LTl0 faiied to react

i n ~ ~ ~ u n o l o c awl iltyh no~tox i rs ubstances, nerue growth factor and collagen.

Currently, the mouse bioassay is sta~dard practice for detection and assay of

tox%ns. Binding afJinity of anti-LTlO, measured as the ELISA detection h i t ,

showed a linear relationship ruith the mouse bioassay. In addition, anti-LTlO

detects toxins that are not lethal to rnice. Th,us, anti-LTl O ,muy poue useful in

assaying toxins as an alternative to ,mouse bioassay.

Dirección del Autor: 

Ophidia Products, Inc., Bellaire, Texas, USA

Keywords: 
lethal toxin neutralizing factor
ELISA
toxin aiitidote
antivenoni
AdjuntoTamaño
[file] Toxin_Reviews_2008_v27_num2_pp125-138_0.pdf384.91 KB