Chemical Decomposition of Glutamine in Cell Culture Media: Effect of Media Type, pH, and Serum Concentration

By jpeza - Posted on 25 Junio 2009

Fecha Publicación: 
1 Ene 1990
Nombre de Revista: 
Datos del paper
Autor Principal: 
Sadettin S. Ozturk
Página Inicial: 
Página Final: 

The chemical decomposition of glutamine to ammonia and pyrrolidonecarboxylic acid

was studied at 37 "C in a pH range of 6.8-7.8 in different media preparations containing

various amounts of fetal bovine serum. The media type influenced the decomposition

rate, and the first-order rate constants increased with increasing pH values.

The serum concentration had little or no effect on the decomposition rate. The importance

of chemical decomposition of glutamine on the analysis of glutamine and ammonia

metabolism was illustrated by an example of batch cultivation of a hybridoma cell

line. The difference between the apparent uptake rate of glutamine and the actual

uptake rate (which is corrected for the chemical decomposition) is shown to be as high

as 200%. Similar discrepancy between the apparent and actual ammonia production

rate is observed. Mathematical analysis was carried out to develop the relationship

between the apparent and actual glutamine uptake and ammonia production rates.

The analysis reveals that there are three important dimensionless parameter ratios

that govern the difference between the apparent and actual glutamine uptake and

ammonia production rates.

Dirección del Autor: 

Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109

fetal bovine serum ; chemical decomposition ; ammonia ; glutamine ; cultivation of a hybridoma cell

Bernhard 0. Palsson

[file] biotechnology_progress_1990_vol.6_pp 121-128.pdf840.89 KB