Re-InfectionProfileof Baculovirusesto 8f-9 InsectCellsthat Have


By jpeza - Posted on 28 Mayo 2009

Fecha Publicación: 
1 Ene 2008
Datos del paper
Autor Principal: 
Takeshi GOTOH
Volumen: 
41
Issue: 
8
Página Inicial: 
804
Página Final: 
808
Abstract: 

Baculovirus re-infection to already-infected insect cells was assessed by comparing the binding of

radio-Iabeled AcMNPV to Sf-9 cells with the expression of marker proteins, ,B-galactosidase and variant

of green fluorescent protein, respectively coded in the baculoviruses. The binding of baculoviruses to

virus-infected Sf-9 insect cells was signifi~~ntly inhibited with the elapse of time post primary infection

(ppi) and completely inhibited by 12 h-ppi. The protein analysis suggested that baculoviruses bound to

already-infected cells were internalized into the cells and routed for protein expression. However, the

yield of protein coded in 2nd viruses (for re-infection) decreased with delaying re-infection time from the

infection with primary viruses, and the expression of 2nd virus protein was not determined on the reinfection

at 12 h-ppi. On the contrary, the expression of primary virus protein was inhibited by bringing

forward the time ofre-infection with 2nd viruses, giving nearly a steady level oftotal volumetric productivity.

Dirección del Autor: 

Department of Engineering in Applied Chemistry, Akita University,

1-1, Tegata Gakuen-cho, Akita-shi, Akita 010-8502, Japan

Keywords: 
Baculovirus
Re-Infection
AcMNPV
Sf-9
Radiolabeling
Coautores: 

NozomiANDO and Ken-Ichi KIKUCHI