Symbiotic plasmid is required for NolR to fully repress nodulation genes in


By jpeza - Posted on 21 Mayo 2009

Fecha Publicación: 
1 Ene 2008
Datos del paper
Autor Principal: 
Fengqing Li
Volumen: 
40
Issue: 
10
Página Inicial: 
901
Página Final: 
907
Abstract: 

NolR is a regulator of nodulation genes present in Rhizobium

and Sinorhizobium. However, the mechanism by which NolR

participates in the inducible transcription of nodulation genes

remains unclear. To investigate whether there are other

factors regulating the function of NolR, an insertion mutant

of NolR in Rhizobium leguminosarum strain 8401, which

lacks the symbiotic plasmid, was constructed by homologous

recombination. We investigated the effects of NolR inactivation

on the expression of nodulation genes. Three inducible

nodulation genes (nodA, nodF and nodM) were expressedconstitutively in NolRmutant, MR114. Our results

suggested that the symbiotic plasmid is required for NolR to

fully repress nodulation genes in Rhizobium leguminosarum

A34. In addition, MR114 has provided a useful tool for further

study of molecular interactions between NolR and other factors

Dirección del Autor: 

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200031, China

Keywords: 
NolR; symbiotic plasmid; nod box
Coautores: 

 Bihe Hou;  Guofan Hong*

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[file] Acta_biochimica_et_biophysica_sinica 2008_vol40_num10_pp901-907.pdf520.58 KB