Interactions between Pro- and Eukaryotes Group

Formación de nuevos lípidos de membrana en rizobia bajo condiciones de estrés

Principal investigator: Christian Sohlenkamp

Participants: Otto Geiger, Isabel María López-Lara, Miguel Angel Vences-Guzmán

Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation

Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation

Participants: Otto Geiger, Christian Sohlenkamp, Ana Laura Ramos-Vega, Yadira Dávila-Martínez, Sandra Leticia Beltrán-Escobar

Biosynthesis of choline-derived bacterial cell surface components...

Biosynthesis of choline-derived bacterial cell surface components: an essential requirement for the success of many pathogenic bacteria


Principal investigator: Otto Geiger

Grupo de Interacciones entre Pro- y Eucariotes

Investigador Responsable Otto Geiger, Ph.D.

The research group

By the time this Research Group joined the Nitrogen Fixation Research Center in 1999, the laboratory of Otto Geiger had discovered the phosphatidylcholine synthase pathway, which constitutes a major route for phosphatidylcholine (lecithine) formation in bacteria. This finding started a research line aimed to understand how molecules of the bacterial membrane or cell surface modify defense responses of eukaryotic hosts thus allowing long-lasting or permanent infections which can lead to symbiotic or pathogenic interactions with the host.


# Año Ficha Ligas
1 2017 López-Lara, I.M. and Geiger, O. (2017). "Bacterial Lipid Diversity". Bba-mol Cell Biol L. 1862(11):1287-1299   PUBMED: #27760387
2 2017 Sandoval-Calderón, M., Guan, Z.Q. and Sohlenkamp, C. (2017). "Knowns and Unknowns of Membrane Lipid Synthesis in Streptomycetes". Biochimie. 141:21-29   PUBMED: #28522365
3 2017 Vences-Guzmán, M.Á., Goetting-Minesky, M.P., Guan, Z.Q., Castillo-Ramírez, S., Cordoba-Castro, L.A., López-Lara, I.M., Geiger, O., Sohlenkamp, C. and Fenno, J.C. (2017). "1,2-Diacylglycerol Choline Phosphotransferase Catalyzes the Final Step In the Unique Treponema denticola Phosphatidylcholine Biosynthesis Pathway". Mol Microbiol. 103(5):896-912   PUBMED: #28009086
4 2016 Sahonero-Canavesi, D.X., Zavaleta-Pastor, M., Martínez-Aguilar, L., López-Lara, I.M. and Geiger, O. (2016). "Defining Substrate Specificities for Lipase and Phospholipase Candidates". Jove-journal of Visualized Experiments. (117)-e54613   PUBMED: #27911408
5 2016 Sohlenkamp, C. and Geiger, O. (2016). "Bacterial Membrane Lipids: Diversity in Structures and Pathways". FEMS Microbiol Rev. 40(1):133-159   PUBMED: #25862689
6 2016 Wang, M.X., Carver, J.J., Phelan, V.V., Sánchez, L.M., Garg, N., Peng, Y., Nguyen, D.D., Watrous, J., Kapono, C.A., Luzzatto-Knaan, T., Porto, C., Bouslimani, A., Melnik, A.V., Meehan, M.J., Liu, W.T., Criisemann, M., Boudreau, P.D., Esquenazi, E., Sandoval-Calderón, M., Kersten, R.D., Pace, L.A., Quinn, R.A., Duncan, K.R., Hsu, C.C., Floros, D.J., Gavilan, R.G., Kleigrewe, K., Northen, T., Dutton, R.J., Parrot, D., Carlson, E.E., Aigle, B., Michelsen, C.F., Jelsbak, L., Sohlenkamp, C., Pevzner, P., Edlund, A., McLean, J., Piel, J., Murphy, B.T., Gerwick, L., Liaw, C.C., Yang, Y.L., Humpf, H.U., Maansson, M., Keyzers, R.A., Sims, A.C., Johnson, A.R., Sidebottom, A.M., Sedio, B.E., Klitgaard, A., Larson, C.B., Boya, C.A., Torres-Mendoza, D., González, D.J., Silva, D.B., Marques, L.M., Demarque, D.P., Pociute, E., ONeill, E.C., Helfrich, E.J.N., Granatosky, E.A., Glukhov, E., Ryffel, F., Houson, H., Mohimani, H., Kharbush, J.J., Zeng, Y., Vorholt, J.A., Kurita, K.L., Charusanti, P., McPhail, K.L., Nielsen, K.F., Vuong, L., Elfeki, M., Traxler, M.F., Engene, N., Koyama, N., Vining, O.B., Baric, R., Silva, R.R., Mascuch, S.J., Tomasi, S., Jenkins, S., Macherla, V., Hoffman, T., Agarwal, V., Williams, P.G., Dai, J.Q., Neupane, R., Gurr, J., Rodríguez, A.M.C., Lamsa, A., Zhang, C., Dorrestein, K., Duggan, B.M., Almaliti, J., Allard, P.M., Phapale, P., Nothias, L.F., Alexandrovr, T., Litaudon, M., Wolfender, J.L., Kyle, J.E., Metz, T.O., Peryea, T., Nguyen, D.T., VanLeer, D., Shinn, P., Jadhav, A., Muller, R., Waters, K.M., Shi, W.Y., Liu, X.T., Zhang, L.X., Knight, R., Jensen, P.R., Palsson, B.O., Pogliano, K., Linington, R.G., Gutiérrez, M., Lopes, N.P., Gerwick, W.H., Moore, B.S., Dorrestein, P.C. and Bandeira, N. (2016). "Sharing and Community Curation of Mass Spectrometry Data with Global Natural Products Social Molecular Networking". Nat Biotechnol. 34(8):828-837   PUBMED: #27504778
7 2015 Aguilar, C., Flores, N., Riveros-McKay, F., Sahonero-Canavesi, D.X., Carmona, S.B., Geiger, O., Escalante, A. and Bolivar, F. (2015). "Deletion of the 2-Acyl-glycerophosphoethanolamine Cycle Improve Glucose Metabolism in Escherichia coli Strains Employed for Overproduction of Aromatic Compounds". Microb Cell Fact. 14(1):194   PUBMED: #26627477
8 2015 Escobedo-Hinojosa, W.I., Vences-Guzmán, M.Á., Schubotz, F., Sandoval-Calderón, M., Summons, R.E., López-Lara, I.M., Geiger, O. and Sohlenkamp, C. (2015). "Olsg (sinac_1600) is an Ornithine Lipid N-methyltransferase from the Planctomycete Singulisphaera acidiphila". J Biol Chem. 290(24):15102-15111   PUBMED: #25925947
9 2015 Sahonero-Canavesi, D.X., Sohlenkamp, C., Sandoval-Calderón, M., Lamsa, A., Pogliano, K., López-Lara, I.M. and Geiger, O. (2015). "Fatty Acid-releasing Activities In Sinorhizobium meliloti Include Unusual Diacylglycerol Lipase". Environ Microbiol. 17(9):3391-3406   PUBMED: #25711932
10 2015 Sandoval-Calderón, M., Nguyen, D.D., Kapono, C.A., Herron, P., Dorrestein, P and Sohlenkamp, C. (2015). "Plasticity Of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions And during Development". Frontiers in Microbiology. 6:1465   PUBMED: #26733994
11 2015 Vences-Guzmán, M.Á., Guan, Z.Q., Escobedo-Hinojosa, W.I., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2015). "Discovery Of A Bifunctional Acyltransferase Responsible for Ornithine Lipid Synthesis in Serratia Proteamaculans". Environ Microbiol. 17(5):1487-1496   PUBMED: #25040623
12 2014 Semeniuk, A., Sohlenkamp, C., Duda, K. and Holzl, G. (2014). "A Bifunctional Glycosyltransferase from Agrobacterium tumefaciens Synthesizes Monoglucosyl and Glucuronosyl Diacylglycerol under Phosphate Deprivation". J Biol Chem. 289(14):10104-10114   PUBMED: #24558041
13 2013 Abdel-Haliem, M.E.F., Sakr, A.A., Ali, M.F., Ghaly, M.F. and Sohlenkamp, C. (2013). "Characterization of Streptomyces Isolates Causing Colour Changes of Mural Paintings in Ancient Egyptian Tombs". Microbiological Research. 168(7):428-437   PUBMED: #23507492
14 2013 Geiger, O., López-Lara, I.M. and Sohlenkamp, C. (2013). "Phosphatidylcholine Biosynthesis and Function in Bacteria". Bba-mol Cell Biol L. 1831(3):503-513   PUBMED: #22922101
15 2013 Hernández-Tamayo, R., Sohlenkamp, C., Puente, J.L., Brom, S. and Romero, D. (2013). "Characterization of Inta, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42". J Bacteriol. 195(20):4668-4677   PUBMED: #23935046
16 2013 Sohlenkamp, C., Raetz, C.R.H. and Ingram, B.O. (2013). "The Calcium-stimulated lipid A 3-o Deacylase from Rhizobium etli is not Essential for Plant Nodulation". Bba-mol Cell Biol L. 1831(7):1250-1259   PUBMED: #24046865
17 2013 Vences-Guzmán, M.Á., Guan, Z.Q., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2013). "Agrobacteria Lacking Ornithine Lipids Induce More Rapid Tumour Formation". Environ Microbiol. 15(3):895-906   PUBMED: #22958119
18 2012 Estrada-de los Santos, P., Martínez-Aguilar, L., López-Lara, I.M. and Caballero-Mellado, J. (2012). "Cupriavidus Alkaliphilus sp nov., A New Species Associated With Agricultural Plants that Grow in Alkaline Soils". Syst Appl Microbiol. 35(5):310-314   PUBMED: #22738764
19 2012 Higareda, M.F., Geiger, O., Mendoza, L. and Mendez-Sánchez, R.A. (2012). "Evidence of Codon Usage in the Nearest Neighbor Spacing Distribution of Bases in Bacterial Genomes". Physica a. 391(4):1255-1269    
20 2012 Solis-Oviedo, R.L., Martínez-Morales, F., Geiger, O. and Sohlenkamp, C. (2012). "Functional And Topological Analysis of Phosphatidylcholine Synthase from Sinorhizobium meliloti". Bba-mol Cell Biol L. 1821(4):573-581   PUBMED: #22333179
21 2012 Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2012). "Ornithine Lipids and their Structural Modifications: from a to e and beyond". FEMS Microbiol Lett. 335(1):1-10   PUBMED: #22724388
22 2011 González-Silva, N., López-Lara, I.M., Reyes-Lamothe, R., Taylor, A.M., Sumpton, D., Thomas-Oates, J. and Geiger, O. (2011). "The Dioxygenase-encoding Olsd Gene from Burkholderia cenocepacia Causes the Hydroxylation of the Amide-linked Fatty Acyl Moiety of Ornithine-containing Membrane Lipids". Biochemistry-us. 50(29):6396-6408   PUBMED: #21707055
23 2011 Landeta, C., Dávalos, A., Cevallos, M.A., Geiger, O., Brom, S. and Romero, D. (2011). "Plasmids With a Chromosome-like Role in Rhizobia". J Bacteriol. 193(6):1317-1326   PUBMED: #21217003
24 2011 Pech-Canul, A., Nogales, J., Miranda-Molina, A., Álvarez, L., Geiger, O., Soto, M.J. and López-Lara, I.M. (2011). "FadD is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids". J Bacteriol. 193(22):6295-6304   PUBMED: #21926226
25 2011 Vences-Guzmán, M.Á., Guan, Z.Q., Ormeño-Orrillo, E., González-Silva, N., López-Lara, I.M., Martínez-Romero, E., Geiger, O. and Sohlenkamp, C. (2011). "Hydroxylated Ornithine Lipids Increase Stress Tolerance In Rhizobium tropici CIAT899". Mol Microbiol. 79(6):1496-1514   PUBMED: #21205018
26 2010 Dávila-Martínez, Y., Ramos-Vega, A.L., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2010). "Smc01553 Is The Sixth Acyl Carrier Protein in Sinorhizobium meliloti 1021". Microbiology-sgm. 156:230-239   PUBMED: #19797355
27 2010 Geiger, O., González-Silva, N., López-Lara, I.M. and Sohlenkamp, C. (2010). "Amino Acid-containing Membrane Lipids in Bacteria". Prog Lipid Res.   PUBMED: #19703488
28 2010 Ingram, B.O., Sohlenkamp, C., Geiger, O. and Raetz, C.R.H. (2010). "Altered lipid A Structures and Polymyxin Hypersensitivity of Rhizobium etli Mutants Lacking the lpxE and LpxF Phosphatases". Bba-mol Cell Biol L. 1801(5):593-604   PUBMED: #20153447
29 2010 Medeot, D.B., Sohlenkamp, C., Dardanelli, M.S., Geiger, O., de Lema, M.G. and López-Lara, I.M. (2010). "Phosphatidylcholine Levels Of Peanut-nodulating Bradyrhizobium sp SEMIA 6144 Affect Cell Size and Motility". FEMS Microbiol Lett. 303(2):123-131   PUBMED: #20030724
30 2010 Zavaleta-Pastor, M., Sohlenkamp, C., Gao, J.L., Guan, Z.Q., Zaheer, R., Finan, T.M., Raetz, C.R.H., López-Lara, I.M. and Geiger, O. (2010). "Sinorhizobium meliloti Phospholipase C Required For Lipid Remodeling during Phosphorus Limitation". P Nat Acad Sci USA. 107(1):302-307   PUBMED: #20018679
31 2009 Ramos-Vega, A.L., Dávila-Martínez, Y., Sohlenkamp, C., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2009). "SMb20651 Is Another Acyl Carrier Protein from Sinorhizobium meliloti". Microbiology. 155:257-267   PUBMED: #19118366
32 2009 Sandoval-Calderón, M., Geiger, O., Guan, Z. Q., Barona-Gómez, F. and Sohlenkamp, C. (2009). "A Eukaryote-like Cardiolipin Synthase is Present in Streptomyces coelicolor and in Most Actinobacteria". J Biol Chem. 284(26):17383-17390   PUBMED: #19439403
33 2008 Conover, G.M., Martinez-Morales, F., Heidtman, M.I., Luo, Z.Q., Tang, M., Chen, C., Geiger, O. and Isberg, R.R. (2008). "Phosphatidylcholine Synthesis Is Required for Optimal Function of Legionella pneumophila Virulence Determinants". Cell Microbiol. 10(2):514-528   PUBMED: #17979985
34 2008 Hacker, S., Sohlenkamp, C., Aktas, M., Geiger, O. and Narberhaus, F. (2008). "Multiple Phospholipid N-Methyltransferases With Distinct Substrate Specificities Are Encoded in Bradyrhizobium japonicum". J Bacteriol. 190(2):571-580   PUBMED: #17993534
35 2008 Sepúlveda, E., Perez-Mendoza, D., Ramírez-Romero, M.Á., Soto, M.J., López-Lara, I.M., Geiger, O., Sanjuan, J., Brom, S. and Romero, D. (2008). "Transcriptional Interference And Repression Modulate the Conjugative Ability of the Symbiotic Plasmid of Rhizobium etli". J Bacteriol. 190(12):4189-4197   PUBMED: #18424522
36 2008 Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2008). "Sinorhizobium meliloti Mutants Deficient In Phosphatidylserine Decarboxylase Accumulate Phosphatidylserine And are Strongly Affected during Symbiosis with Alfalfa". J Bacteriol. 190(20):6846-6856   PUBMED: #18708506
37 2007 Medeot, D.B., Sohlenkamp, C., Geiger, O., García, M.B. and López-Lara, I.M. (2007). "Phosphatidycholine Fuction in Rhizobial Strains Nodulating Peanut: Genes and Enzymatic Activites". Biocell. 31:134    
38 2007 Sohlenkamp, C., Galindo-Lagunas, K.A., Guan, Z.Q., Vinuesa, P., Robinson, S., Thomas-Oates, J., Raetz, C.R.H. and Geiger, O. (2007). "The Lipid Lysyl-phosphatidylglycerol Is Present In Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B under Acidic Growth Conditions". Mol Plant Microbe Interactions. 20(11):1421-1430   PUBMED: #17977153
39 2006 López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2006). "The Post-genomic Era--what Comes next?". Revista Latinoamericana de Microbiología. 48(1):44-45   PUBMED: #17357573
40 2006 Reeve, W.G., Brau, L., Castelli, J., Garau, G., Sohlenkamp, C., Geiger, O., Dilworth, M.J., Glenn, A.R., Howieson, J.G. and Tiwaril, R.P. (2006). "The Sinorhizobium Medicae WSM419 Ipia Gene is Transcriptionally Activated by FsrR and Required to Enhance Survival in Lethal Acid Conditions". Microbiology-sgm. 152:3049-3059   PUBMED: #17005985
41 2005 López-Lara, I.M., Gao, J.L., Soto, M.J., Solares-Pérez, A., Weissenmayer, B., Sohlenkamp, C., Verroios, G.P., Thomas-Oates, J. and Geiger, O. (2005). "Phosphorus-free Membrane Lipids of Sinorhizobium meliloti are not Required for the Symbiosis with Alfalfa but Contribute to Increased Cell Yields under Phosphorus-limiting Conditions of Growth". Mol Plant Microbe Interactions. 18(9):973-982   PUBMED: #16167767
42 2005 Rojas-Jimenez, K., Sohlenkamp, C., Geiger, O., Martínez-Romero, E., Werner, D. and Vinuesa, P. (2005). "A Cic Chloride Channel Homolog and Ornithine-containing Membrane Lipids of Rhizobium tropici CIAT899 are Involved in Symbiotic Efficiency and Acid Tolerance". Mol Plant Microbe Interactions. 18(11):1175-1185   PUBMED: #16353552
43 2004 Gao, J.L., Weissenmayer, B., Taylor, A.M., Thomas-Oates, J., López-Lara, I.M. and Geiger, O. (2004). "Identification of a Gene Required for the Formation of Lyso-ornithine Lipid, an Intermediate in the Biosynthesis of Ornithine-containing Lipids". Mol Microbiol. 53(6):1757-1770   PUBMED: #15341653
44 2004 Sohlenkamp, C., de Rudder, K.E.E. and Geiger, O. (2004). "Phosphatidylethanolamine Is not Essential for Growth of Sinorhizobium meliloti on Complex Culture Media". J Bacteriol. 186(6):1667-1677   PUBMED: #14996797
45 2003 Djordjevic, M.A., Chen, H.C., Natera, S., Van Noorden, G., Menzel, C., Taylor, S., Renard, C., Geiger, O. and Weiller, G.F. (2003). "A Global Analysis of Protein Expression Profiles in Sinorhizobium meliloti: Discovery of New Genes for Nodule Occupancy and Stress Adaptation". Mol Plant Microbe Interactions. 16(6):508-524   PUBMED: #12795377
46 2003 López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2003). "Membrane Lipids in Plant-associated Bacteria: their Biosyntheses and Possible Functions". Mol Plant Microbe Interactions. 16(7):567-579   PUBMED: #12848422
47 2003 Martínez-Morales, F., Schobert, M., López-Lara, I.M. and Geiger, O. (2003). "Pathways for Phosphatidylcholine Biosynthesis in Bacteria". Microbiology-sgm. 149:3461-3471   PUBMED: #14663079
48 2003 Sohlenkamp, C., López-Lara, I.M. and Geiger, O. (2003). "Biosynthesis of Phosphatidylcholine in Bacteria". Prog Lipid Res. 42(2):115-162   PUBMED: #12547654
49 2002 Geiger, O. and López-Lara, I.M. (2002). "Rhizobial Acyl Carrier Proteins and their Roles in the Formation of Bacterial Cell-surface Components that are Required for the Development of Nitrogen-fixing Root Nodules on Legume Hosts". FEMS Microbiol Lett. 208(2):153-162   PUBMED: #11959430
50 2002 Sohlenkamp, C., Wood, C.C., Roeb, G.W. and Udvardi, M.K. (2002). "Characterization of Arabidopsis AtAMT2, A High-affinity Ammonium Transporter of the Plasma Membrane". Plant Physiol. 130(4):1788-1796   PUBMED: #12481062
51 2002 Weissenmayer, B., Gao, J.L., López-Lara, I.M. and Geiger, O. (2002). "Identification of a Gene Required for the Biosynthesis of Ornithine-derived Lipids". Mol Microbiol. 45(3):721-733   PUBMED: #12139618
52 2001 López-Lara, I.M. and Geiger, O. (2001). "Novel Pathway for Phosphatidylcholine Biosynthesis in Bacteria Associated with Eukaryotes". J Biotechnol. 91(2-3):211-221   PUBMED: #11566392
53 2001 López-Lara, I.M. and Geiger, O. (2001). "The Nodulation Protein NodG Shows the Enzymatic Activity of an 3-Oxoacyl-Acyl Carrier Protein Reductase". Mol Plant Microbe Interactions. 14(3):349-357   PUBMED: #11277432
54 2001 Minder, A.C., de Rudder, K.E.E., Narberhaus, F., Fischer, H.M., Hennecke, H. and Geiger, O. (2001). "Phosphatidylcholine Levels In Bradyrhizobium japonicum Membranes Are Critical for an Efficient Symbiosis with the Soybean Host Plant". Mol Microbiol. 39(5):1186-1198   PUBMED: #11251836
55 2000 de Rudder, K.E.E., López-Lara, I.M. and Geiger, O. (2000). "Inactivation Of the Gene for Phospholipid N-methyltransferase in Sinorhizobium meliloti: Phosphatidylcholine is Required for Normal Growth". Mol Microbiol. 37(4):763-772   PUBMED: #10972799
56 2000 Sohlenkamp, C., de Rudder, K.E.E., Rohrs, V., López-Lara, I.M. and Geiger, O. (2000). "Cloning and Characterization of the Gene for Phosphatidylcholine Synthase". J Biol Chem. 275(25):18919-18925   PUBMED: #10858449
57 2000 Weissenmayer, B., Geiger, O. and Benning, C. (2000). "Disruption Of a Gene Essential for Sulfoquinovosyldiacylglycerol Biosynthesis in Sinorhizobium meliloti Has no Detectable Effect on Root Nodule Symbiosis". Mol Plant Microbe Interactions. 13(6):666-672   PUBMED: #10830266

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