Soil and Agricultural Microbiology Group

Premio Agrobio 2008 para el Dr. Caballero

El Dr. Jesús Caballero Mellado, Investigador Titular C del Centro de Ciencias Genómicas de la UNAM, recibió el pasado 23 de Octubre el Premio Agrobio México 2008, en la categoría de Investigación en Biotecnología Agrícola.

Grupo de Microbiología del Suelo y Agrícola

Investigador Responsable Jesus Caballero-Mellado, Ph.D.

Main lines of Research: Taxonomy, diverstity and ecology of nitrogen-fixing bacteria associated with crop plants and their use in agriculture.

The research group

This Group was created in September 1995 in the Nitrogen Fixation Research Center (CIFN) with the objective of contributing to the improvement of sustainable agriculture through the basic and applied research in biological nitrogen fixation (BNF). Our studies have been directed at looking for well-known N 2 -fixing species (for instance, Azospirillum and Gluconacetobacter ), and for new rhizospheric and endophytic diazotrophs associated with plants of agricultural importance (e.g. corn and sugarcane) as well as "neglected" plants [e.g., coffee and teocintle (maize ancestor)], because it may provide a better picture not only of the distribution of N 2 -fixation ability among bacterial taxa, but also of the distribution and diversity of N 2 -fixing bacterial populations. For this, we have searched culture conditions for the selective isolation and definition of the taxonomic status of novel diazotrophs, using classic and molecular criteria, with the final objective of evaluating their potential to promote plant growth and increase yield of cereals, especially of maize.


# Año Ficha Ligas
1 2016 Sahonero-Canavesi, D.X., Zavaleta-Pastor, M., Martínez-Aguilar, L., López-Lara, I.M. and Geiger, O. (2016). "Defining Substrate Specificities for Lipase and Phospholipase Candidates". Jove-journal of Visualized Experiments. (117)-e54613   PUBMED: #27911408
2 2014 Estrada-de los Santos, P., Solano-Rodríguez, R., Matsumura-Paz, L.T., Vasquez-Murrieta, M.S. and Martínez-Aguilar, L. (2014). "Cupriavidus Plantarum sp. nov., A Plant-associated Species". Arch Microbiol. 196(11):811-817   PUBMED: #25098225
3 2013 Estrada-de los Santos, P., Vinuesa, P., Martínez-Aguilar, L., Hirsch, A.M. and Caballero-Mellado, J. (2013). "Phylogenetic Analysis Of Burkholderia Species By multilocus Sequence Analysis". Curr Microbiol. 67(1):51-60   PUBMED: #23404651
4 2013 Martínez-Aguilar, L., Caballero-Mellado, J. and Estrada-de los Santos, P. (2013). "Transfer Of Wautersia Numazuensis To The Genus Cupriavidus As Cupriavidus Numazuensis Comb. Nov.". Int J Syst Evol Microbiol. 63:208-211   PUBMED: #22389284
5 2013 Martínez-Aguilar, L., Salazar-Salazar, C., Díaz-Mendez, R., Caballero-Mellado, J., Hirsch, A.M., Vasquez-Murrieta, M.S. and Estrada-de los Santos, P. (2013). "Burkholderia Caballeronis sp nov., A Nitrogen Fixing Species Isolated From Tomato (Lycopersicon esculentum) With The Ability To Effectively Nodulate Phaseolus vulgaris". Anton Leeuwenhoek Int J Gen M. 104(6):1063-1071   PUBMED: #24057982
6 2012 Estrada-de los Santos, P., Martínez-Aguilar, L., López-Lara, I.M. and Caballero-Mellado, J. (2012). "Cupriavidus Alkaliphilus sp nov., A New Species Associated With Agricultural Plants that Grow in Alkaline Soils". Syst Appl Microbiol. 35(5):310-314   PUBMED: #22738764
7 2011 Castro-González, R., Martínez-Aguilar, L., Ramírez-Trujillo, J.A., Estrada-de los Santos, P. and Caballero-Mellado, J. (2011). "High Diversity Of Culturable Burkholderia Species Associated with Sugarcane". Plant Soil. 345(1-2):155-169    
8 2011 Estrada-de los Santos, P., Vacaseydel-Aceves, N.B., Martínez-Aguilar, L., Mendoza-Herrera, A. and Caballero-Mellado, J. (2011). "Cupriavidus And Burkholderia Species Associated With Agricultural Plants that Grow in Alkaline Soils". J Microbiol. 49(6):867-876   PUBMED: #22203548
9 2011 Gyaneshwar, P., Hirsch, A.M., Moulin, L., Chen, W.M., Elliott, G.N., Bontemps, C., Estrada-de los Santos, P., Gross, E., Dos Reis, F.B., Sprent, J.I., Young, J.P. and James, E.K. (2011). "Legume-nodulating Betaproteobacteria: Diversity, Host Range, and Future Prospects". Mol Plant Microbe Interactions. 24(11):1276-1288   PUBMED: #21830951
10 2010 Couillerot, O., Poirier, M.A., Prigent-Combaret, C., Mavingui, P., Caballero-Mellado, J. and Moenne-Loccoz, Y. (2010). "Assessment of SCAR Markers to Design Real-time PCR Primers for Rhizosphere Quantification of Azospirillum brasilense Phytostimulatory Inoculants of Maize". J. Appl Microbiol. 109(2):528-538   PUBMED: #20141548
11 2010 Couillerot, O., Bouffaud, M.L., Baudoin, E., Muller, D., Caballero-Mellado, J. and Moenne-Loccoz, Y. (2010). "Development Of a Real-time PCR Method to Quantify the PGPR Strain Azospirillum Lipoferum CRT1 on Maize Seedlings". Soil Biol Biochem. 42(12):2298-2305    
12 2010 Marin-Cevada, V., Caballero-Mellado, J., Bustillos-Cristales, R., Muñoz-Rojas, J., Mascarua-Esparza, M.A., Castaneda-Lucio, M., Lopez-Reyes, L., Martínez-Aguilar, L. and Fuentes-Ramirez, L.E. (2010). "Tatumella Ptyseos, an Unrevealed Causative Agent of Pink Disease in Pineapple". J Phytopathol. 158(2):93-99    
13 2010 Suarez-Moreno, Z.R., Devescovi, G., Myers, M., Hallack, L., Mendonca-Previato, L., Caballero-Mellado, J. and Venturi, V. (2010). "Commonalities And Differences in Regulation of N-Acyl Homoserine Lactone Quorum Sensing in the Beneficial Plant-associated Burkholderia Species Cluster". Appl Environ Microbiol. 76(13):4302-4317   PUBMED: #20435760
14 2010 Talbi, C., Delgado, M.J., Girard, L., Ramírez-Trujillo, J.A., Caballero-Mellado, J. and Bedmar, E.J. (2010). "Burkholderia Phymatum Strains Capable Of Nodulating Phaseolus vulgaris Are Present In Moroccan Soils". Appl Environ Microbiol. 76(13):4587-4591   PUBMED: #20472732
15 2010 Wong-Villarreal, A. and Caballero-Mellado, J. (2010). "Rapid Identification Of Nitrogen-fixing And Legume-nodulating Burkholderia Species Based on PCR 16S rRNA Species-Specific Oligonucleotides". Syst Appl Microbiol. 33(1):35-43   PUBMED: #19945811
16 2009 Couillerot, O, Prigent-Combaret, C, Caballero-Mellado, J. and Moenne-Loccoz, Y (2009). "Pseudomonas fluorescens And Closely-related Fluorescent Pseudomonads as Biocontrol Agents of Soil-borne Phytopathogens". Lett Appl Microbiol. 48(5):505-512   PUBMED: #19291210
17 2009 Dunn, M.F., Ramírez-Trujillo, J.A. and Hernández-Lucas, I. (2009). "Major Roles of Isocitrate Lyase and Malate Synthase in Bacterial and Fungal Pathogenesis". Microbiology-sgm. 155:3166-3175   PUBMED: #19684068
18 2009 Escalante, A. E., Caballero-Mellado, J., Martínez-Aguilar, L., Rodríguez-Verdugo, A., González-González, A., Toribio-Jiménez, J. and Souza, V. (2009). "Pseudomonas Cuatrocienegasensis sp. nov., Isolated From an Evaporating Lagoon in the Cuatro Ciénegas Valley in Coahuila, Mexico". Int J Syst Evol Microbiol. 59:1416-1420   PUBMED: #19502326
19 2009 Menéndez, C., Banguela, A., Caballero-Mellado, J. and Hernández, L. (2009). "Transcriptional Regulation And Signal-peptide-dependent Secretion Of Exolevanase (LsdB) in the Endophyte Gluconacetobacter diazotrophicus". Appl Environ Microbiol. 75(6):1782-1785   PUBMED: #19139238
20 2009 Onofre-Lemus, J., Hernández-Lucas, I., Girard, L. and Caballero-Mellado, J. (2009). "Acc (1-aminocyclopropane-1-carboxylate) Deaminase Activity, a Widespread Trait in Burkholderia Species, and Its Growth-promoting Effect on Tomato Plants". Appl Environ Microbiol. 75(20):6581-6590   PUBMED: #19700546
21 2009 Rodríguez-Salazar, J., Suárez, R., Caballero-Mellado, J. and Iturriaga, G. (2009). "Trehalose Accumulation in Azospirillum brasilense Improves Drought Tolerance and Biomass in Maize Plants". FEMS Microbiol Lett. 296(1):52-59   PUBMED: #19459961
22 2008 Martínez-Aguilar, L., Díaz-Mendez, R., Pena-Cabriales, J.J., Estrada-de los Santos, P., Dunn, M.F. and Caballero-Mellado, J. (2008). "Multichromosomal Genome Structure And Confirmation of Diazotrophy in Novel Plant-associated Burkholderia Species". Appl Environ Microbiol. 74(14):4574-4579   PUBMED: #18502926
23 2008 Suárez, R., Wong-Villarreal, A., Ramirez, M., Barraza, A., Orozco, M.D., Cevallos, M.A., Lara-Flores, M., Hernández, G. and Iturriaga, G. (2008). "Improvement Of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-phosphate Synthase in Rhizobia". Mol Plant Microbe Interactions. 21(7):958-966   PUBMED: #18533836
24 2008 Suarez-Moreno, Z.R., Caballero-Mellado, J. and Venturi, V. (2008). "The New Group of Non-pathogenic Plant-associated Nitrogen-fixing Burkholderia spp. Shares a Conserved Quorum-sensing System, Which is Tightly Regulated by the RsaL Repressor". Microbiology-sgm. 154:2048-2059   PUBMED: #18599833
25 2007 Caballero-Mellado, J., Onofre-Lemus, J., Estrada-de los Santos, P. and Martínez-Aguilar, L. (2007). "The Tomato Rhizosphere, an Environment Rich in Nitrogen-fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation". Appl Environ Microbiol. 73(16):5308-5319   PUBMED: #17601817
26 2007 Granados-Baeza, M.J., Gómez-Hernández, N., Mora, Y., Delgado, M.J., Romero, D. and Girard, L. (2007). "Novel Reiterated Fnr-type Proteins Control the Production of the Symbiotic Terminal Oxidase Cbb(3) in Rhizobium etli CFN42". Mol Plant Microbe Interactions. 20(10):1241-1249   PUBMED: #17918626
27 2007 Menard, A., Monnez, C., Estrada-de los Santos, P., Segonds, C., Caballero-Mellado, J., LiPuma, J.J., Chabanon, G. and Cournoyer, B. (2007). "Selection of Nitrogen-fixing Deficient Burkholderia vietnamiensis Strains By Cystic Fibrosis Patients: Involvement of nif Gene Deletions and Auxotrophic Mutations". Environ Microbiol. 9(5):1176-1185   PUBMED: #17472633
28 2007 Ramírez-Trujillo, J.A., Encarnación, S., Salazar, E., García-de los Santos, A., Dunn, M.F., Emerich, D.W., Calva, E. and Hernández-Lucas, I. (2007). "Functional Characterization Of The Sinorhizobium meliloti Acetate Metabolism Genes aceA, SMc00767, and glcB". J Bacteriol. 189(16):5875-5884   PUBMED: #17526694
29 2006 Caballero-Mellado, J. (2006). "Agriculture Microbiology and Microbe Interaction with Plants". Revista Latinoamericana de Microbiología. 48(2):154-161   PUBMED: #17578087
30 2006 Hernández-Lucas, I., Ramírez-Trujillo, J.A., Gaitan, M.A., Guo, X.W., Flores, M., Martínez-Romero, E., Perez-Rueda, E. and Mavingui, P. (2006). "Isolation And Characterization of Functional Insertion Sequences of Rhizobia". FEMS Microbiol Lett. 261(1):25-31   PUBMED: #16842354
31 2006 Marin-Cevada, V., Vargas, V.H., Juarez, M., Lopez, V.G., Zagada, G., Hernandez, S., Cruz, A., Caballero-Mellado, J., Lopez-Reyes, L., Jimenez-Salgado, T., Carcano-Montiel, M. and Fuentes-Ramirez, L.E. (2006). "Presence of Pantoea Citrea, Causal Agent of Pink Disease, in Pineapple Fields in Mexico". Plant Pathol. 55(2):294-294   PUBMED: #320862
32 2006 Perin, L., Martínez-Aguilar, L., Castro-González, R., Estrada-de los Santos, P., Cabellos-Avelar, T., Guedes, H.V., Reis, V.M. and Caballero-Mellado, J. (2006). "Diazotrophic Burkholderia Species Associated With Field-Grown Maize And Sugarcane". Appl Environ Microbiol. 72(5):3103-3110   PUBMED: #16672447
33 2006 Perin, L., Martínez-Aguilar, L., Paredes-Valdez, G., Baldani, J.I., Estrada-de los Santos, P., Reis, V.M. and Caballero-Mellado, J. (2006). "Burkholderia silvatlantica sp. nov., A Diazotrophic Bacterium Associated with Sugar Cane and Maize". Int J Syst Evol Microbiol. 56:1931-1937   PUBMED: #16902033
34 2005 Bueno, E., Gómez-Hernández, N., Girard, L., Bedmar, E.J. and Delgado, M.J. (2005). "Function Of the Rhizobium etli CFN42 nirK Gene in Nitrite Metabolism". Biochem Soc Trans. 33:162-163   PUBMED: #15667294
35 2005 Muñoz-Rojas, J., Fuentes-Ramirez, L.E. and Caballero-Mellado, J. (2005). "Antagonism Among Gluconacetobacter diazotrophicus Strains in Culture Media And in Endophytic Association". FEMS Microbiol Ecol. 54(1):57-66   PUBMED: #16329972
36 2005 Muthukumarasamy, R., Cleenwerck, I., Revathi, G., Vadivelu, M., Janssens, D., Hoste, B., Gum, K.U., Park, K.D., Son, C.Y., Sa, T. and Caballero-Mellado, J. (2005). "Natural Association Of Gluconacetobacter diazotrophicus And Diazotrophic Acetobacter Peroxydans With Wetland Rice". Syst Appl Microbiol. 28(3):277-286   PUBMED: #15900973
37 2005 Valdes, M., Perez, N.O., Estrada-de los Santos, P., Caballero-Mellado, J., Pena-Cabriales, J.J., Normand, P. and Hirsch, A.M. (2005). "Non-Frankia Actinomycetes Isolated From Surface-sterilized Roots Of casuarina equisetifolia Fix Nitrogen". Appl Environ Microbiol. 71(1):460-466   PUBMED: #15640222
38 2004 Caballero-Mellado, J., Martínez-Aguilar, L., Paredes-Valdez, G. and Estrada-de los Santos, P. (2004). "Burkholderia unamae sp. nov., An N-2-fixing Rhizospheric and Endophytic Species". Int J Syst Evol Microbiol. 54:1165-1172   PUBMED: #15280286
39 2004 Goris, J., De Vos, P., Caballero-Mellado, J., Park, J., Falsen, E., Quensen, J.F., Tiedje, J.M. and Vandamme, P. (2004). "Classification Of The Biphenyl- and Polychlorinated Biphenyl-degrading Strain LB400(t) and Relatives as Burkholderia xenovorans sp. nov.". Int J Syst Evol Microbiol. 54:1677-1681   PUBMED: #15388727
40 2004 Reis, V.M., Estrada-de los Santos, P., Tenorio-Salgado, S., Vogel, J., Stoffels, M., Guyon, S., Mavingui, P., Baldani, V.L.D., Schmid, M., Baldani, J.I., Balandreau, J., Hartmann, A. and Caballero-Mellado, J. (2004). "Burkholderia Tropica sp. nov., A Novel Nitrogen-fixing, Plant-associated Bacterium". Int J Syst Evol Microbiol. 54:2155-2162   PUBMED: #15545451
41 2003 Martínez, L., Caballero-Mellado, J., Orozco, J. and Martínez-Romero, E. (2003). "Diazotrophic Bacteria Associated with Banana (Musa spp.).". Plant Soil. 257(1):35-47    
42 2003 Muñoz-Rojas, J. and Caballero-Mellado, J. (2003). "Population Dynamics Of Gluconacetobacter diazotrophicus In Sugarcane Cultivars and Its Effect on Plant Growth". Microbial Ecol. 46(4):454-464   PUBMED: #14722690
43 2002 Estrada-de los Santos, P., Mavingui, P., Cournoyer, B., Fontaine, F., Balandreau, J. and Caballero-Mellado, J. (2002). "A N-2-fixing Endophytic Burkholderia sp. Associated with Maize Plants Cultivated in Mexico". Can J Microbiol. 48(4):285-294   PUBMED: #12030700
44 2001 Dobbelaere, S., Croonenborghs, A., Thys, A., Ptacek, D., Vanderleyden, J., Dutto, P., Labandera-Gonzalez, C., Caballero-Mellado, J., Aguirre, J.F., Kapulnik, Y., Brener, S., Burdman, S., Kadouri, D., Sarig, S. and Okon, Y. (2001). "Responses Of Agronomically Important Crops to Inoculation with Azospirillum". Aust J Plant Physiol. 28(9):871-879    
45 2001 Estrada-de los Santos, P., Bustillos-Cristales, R. and Caballero-Mellado, J. (2001). "Burkholderia, A Genus Rich in Plant-associated Nitrogen Fixers with Wide Environmental and Geographic Distribution". Appl Environ Microbiol. 67(6):2790-2798   PUBMED: #11375196
46 2001 Fuentes-Ramírez, L.E., Bustillos-Cristales, R., Tapia-Hernández, A., Jiménez-Salgado, T., Wang, E.T., Martínez-Romero, E. and Caballero-Mellado, J. (2001). "Novel Nitrogen-fixing Acetic Acid Bacteria, Gluconacetobacter johannae sp. nov. And Gluconacetobacter azotocaptans sp. nov., Associated With Coffee Plants". Int J Syst Evol Microbiol. 51:1305-1314   PUBMED: #11491326
47 2000 Hernández, L., Sotolongo, M., Rosabal, Y., Menéndez, C., Ramírez, R., Caballero-Mellado, J. and Arrieta, J. (2000). "Structural Levansucrase Gene (lsda) Constitutes A Functional Locus Conserved in the Species Gluconacetobacter diazotrophicus". Arch Microbiol. 174(1-2):120-124   PUBMED: #10985751
48 2000 Tapia-Hernández, A., Bustillos-Cristales, M.R., Jiménez-Salgado, T., Caballero-Mellado, J. and Fuentes-Ramírez, L.E. (2000). "Natural Endophytic Occurrence Of Acetobacter diazotrophicus In Pineapple Plants". Microbial Ecol. 39(1):49-55   PUBMED: #10790517
49 1999 Caballero-Mellado, J., López-Reyes, L. and Bustillos-Cristales, R. (1999). "Presence Of 16S rRNA Genes in Multiple Replicons in Azospirillum brasilense". FEMS Microbiol Lett. 178(2):283-288    
50 1999 Caballero-Mellado, J. and Martínez-Romero, E. (1999). "Soil Fertilization Limits The Genetic Diversity of Rhizobium in Bean Nodules". Symbiosis. 26(2):111-121    
51 1999 Fuentes-Ramírez, L.E., Caballero-Mellado, J., Sepulveda, J. and Martínez-Romero, E. (1999). "Colonization Of Sugarcane by Acetobacter diazotrophicus is Inhibited by High N-fertilization". FEMS Microbiol Ecol. 29(2):117-128    
52 1999 Herrera-Cervera, J.A., Caballero-Mellado, J., Laguerre, G., Tichy, H.V., Requena, N., Amarger, N., Martínez-Romero, E., Olivares, J. and Sanjuan, J. (1999). "At Least Five Rhizobial Species Nodulate Phaseolus vulgaris in a Spanish Soil". FEMS Microbiol Ecol. 30(1):87-97    
53 1998 Sievers, M., Schlegel, H.G., Caballero-Mellado, J., Dobereiner, J. and Ludwig, W. (1998). "Phylogenetic Identification of Two Major Nitrogen-fixing Bacteria Associated with Sugarcane". Syst Appl Microbiol. 21(4):505-508   PUBMED: #9924818
54 1997 Jiménez-Salgado, T., Fuentes-Ramírez, L.E., Tapia-Hernández, A., Mascarua-Esparza, M.A., Martínez-Romero, E. and Caballero-Mellado, J. (1997). "Coffea arabica L., A New Host Plant For Acetobacter diazotrophicus, And Isolation of other Nitrogen-fixing Acetobacteria". Appl Environ Microbiol. 63(9):3676-3683   PUBMED: #9293018
55 1996 Martínez-Romero, E. and Caballero-Mellado, J. (1996). "Rhizobium Phylogenies And Bacterial Genetic Diversity". Crit Rev Plant Sci. 15(2):113-140    
56 1995 Caballero-Mellado, J., Fuentes-Ramírez, L.E., Reis, V.M. and Martínez-Romero, E. (1995). "Genetic Structure of Acetobacter diazotrophicus Populations And Identification of a New Genetically Distant Group". Appl Environ Microbiol. 61(8):3008-3013   PUBMED: #16535102
57 1994 Caballero-Mellado, J. and Martínez-Romero, E. (1994). "Limited Genetic Diversity In The Endophytic Sugarcane Bacterium Acetobacter diazotrophicus". Appl Environ Microbiol. 60(5):1532-1537   PUBMED: #16349254