Genome Engineering Program

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Publicaciones


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Programa de Ingeniería Genómica

Program Responsible: Dr. David Romero.

Este programa está dedicado a la comprensión de las fuerzas y mecanismos que han forjado la arquitectura genómica de proteobacterias asociadas a plantas (específicamente Rhizobium sp.), tanto en el corto plazo como con una perspectiva evolutiva.

El objetivo a largo plazo es utilizar este conocimiento para desarrollar nuevas estrategias en ingeniería genómica. Para ese fin, nuestras líneas de investigación inciden en los mecanismos y consecuencias evolutivas de la recombinación homóloga, recombinación sitio-específica durante la conjugación de plásmidos, regulación de la transferencia conjugativa, genómica funcional de plásmidos (incluyendo el análisis de funciones codificadas en plásmidos y el papel de factores sigma extracitoplásmicos en respuestas a estrés), así como sistemática molecular, microevolución y filogeografía. Parte de este conocimiento se está integrando en la generación de nuevos enfoques para la generación de deleciones programadas y mutagénesis global del genoma.

Nuestro trabajo combina el análisis bioinformático con enfoques genéticos y de biología molecular así como de análisis de genética de poblaciones.

Identification of plasmid-encoded genes involved...

Identification of plasmid-encoded genes involved in the adaptation of Rhizobium etli to the rhizosphere of Phaseolus vulgaris using genetic and genomic approaches.

 

Search for the presence of site-specific recombination systems...

Search for the presence of site-specific recombination systems, similar to the one we found on R. etli CFN42 plasmids, on other Rhizobiacea.

Our previous work has shown the participation of a site-specific recombination in the formation or resolution of cointegrates between the symbiotic plasmid and a smaller, self-transmissible plasmid. These cointegrates are important to achieve conjugative transfer of the pSym. Our work will continue with the search of similar elements on other Rhizobiaceae.

Regulation of conjugative transfer of the symbiotic plasmid of Rhizobium etli


In this line, we are studying the mechanisms involved in repression of a conjugative system present on the pSym of R. etli. This system, which employs one repressor gene rctA) also has interactions with another gene product (RctB) whose overproduction apparently overrides the repression imposed by RctA. Characterization of the regions relevant for transcriptional repression as well as purification and analysis of RctA and RctB are the
objectives of this line.

Systematic analysis of Rhizobium plasmids by programmed deletions


Continuing with our interest in functions encoded in Rhizobium plasmids, we are generating a collection of programmed deletions in each of the six large plasmids of Rhizobium etli. Strains resulting from this work will be used in experiments of functional genomics.

Participants: Araceli Dávalos, Alejandro García, Susana Brom, David Romero

Generation of tools for genome engineering in Rhizobium


Genome Engineering is defined as the in vivo modification of the genome, using homologous or site-specific recombination. In this area, we are taking advantage of our previous experience in selection of genome rearrangements. Moreover, we are implementing several tools for

Dr. Alejandro García de los Santos - "Contribuciones al análisis funcional de los plásmidos de Rhizobium etli CFN42"

10 Dic 2007 - 12:00
10 Dic 2007 - 14:00
Etc/GMT-5
Titulo del Seminario / Conferencia: 

"Contribuciones al análisis funcional de los plásmidos de Rhizobium etli CFN42"

Lugar: 

Auditorio "Dr. Guillermo Soberón", Centro de Ciencias Genómicas, UNAM

Institución: 

Centro de Ciencias Genómicas, UNAM

Dr. David Romero - "El genoma de Rhizobium etli: de la fluidez a la función"

28 Mayo 2007 - 12:00
28 Mayo 2007 - 13:00
Etc/GMT-5
Titulo del Seminario / Conferencia: 

"El genoma de Rhizobium etli: de la fluidez a la función"

Dra. Susana Brom - "Transferencia conjugativa y generación de quimeras en Rhizobium"

15 Oct 2007 - 12:00
15 Oct 2007 - 14:00
Etc/GMT-5
Titulo del Seminario / Conferencia: 

"Transferencia conjugativa y generación de quimeras en Rhizobium"

Projects

Program Responsible: Dr. David Romero.