Functional Genomics of Eukaryotes Program


# Año Ficha Ligas
1 2018 Blanc, C., Coluccia, F., LHaridon, F., Torres, M., Ortíz-Berrocal, M., Stahl, E., Reymond, P., Schreiber, L., Nawrath, C., Metraux, J.P. and Serrano, M. (2018). "The Cuticle Mutant eca2 Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects". Mol Plant Microbe Interactions. 31(3):344-355   PUBMED: #29130376
2 2018 Chiozzotto, R., Ramirez, M., Talbi, C., Cominelli, E., Girard, L., Sparvoli, F. and Hernández, G. (2018). "Characterization Of the Symbiotic Nitrogen-fixing Common Bean low phytic acid (lpa1) Mutant Response to Water Stress". Genes. 9(2)    
3 2017 Aragón, W., Reina-Pinto, J.J. and Serrano, M. (2017). "The Intimate Talk between Plants and Microorganisms at the Leaf Surface". J Exp Bot. 68(19):5339-5350   PUBMED: #29136456
4 2017 Iñiguez-Rabago, L.P. and Hernández, G. (2017). "The Evolutionary Relationship between Alternative Splicing and Gene Duplication". Frontiers in Genetics. 8    
5 2017 Iñiguez-Rabago, L.P., Ramirez, M., Barbazuk, W.B. and Hernández, G. (2017). "Identification and Analysis Of Alternative Splicing Events in Phaseolus vulgaris and Glycine max". BMC Genomics. 18:-650   PUBMED: #28830361
6 2017 Ortíz-Berrocal, M., Lozano, L., Sánchez-Flores, A., Nava, N., Hernández, G. and Reddy, P.M. (2017). "Expression In Rice of an Autoactive Variant of Medicago truncatula Dmi3, the Ca+2/calmodulin-dependent Protein Kinase from the Common Symbiotic Pathway Modifies Root Transcriptome and Improves Mycorrhizal Colonization". Plant Biotechnology Reports. 11(5):271-287    
7 2016 Formey, D., Martín-Rogel, J.A., Leija, A., Santana, O., Quinto, C., Cardenas, L. and Hernández, G. (2016). "Regulation Of Small RNAs and Corresponding Targets in Nod Factor-induced Phaseolus vulgaris Root Hair Cells". International Journal of Molecular Sciences. 17(6)    
8 2016 Gkizi, D., Lehmann, S., LHaridon, F., Serrano, M., Paplomatas, E.J., Metraux, J.P. and Tjamos, S.E. (2016). "The Innate Immune Signaling System As A Regulator of Disease Resistance and Induced Systemic Resistance Activity against Verticillium dahliae". Mol Plant Microbe Interactions. 29(4):313-323   PUBMED: #26780421
9 2016 Hossain, Md. S., Shrestha, A., Zhong, S., Miri, M., Austin, R.R., Sato, S., Ross, L., Huebert, T., Tromas, A., Torres-Jerez, I., Tang, Y., Udvardi, M., Murray, J. and Szczyglowski, K. (2016). "Lotus japonicus Nf-ya1 Plays An Essential Role during Nodule Differentiation And Targets Members of the Shi/sty Gene Family". Mol Plant Microbe Interactions. 29(12):950-964    
10 2016 Ramirez, M., Iñiguez-Rabago, L.P., Guerrero, G., Sparvoli, F. and Hernández, G. (2016). "Rhizobium etli Bacteroids Engineered For Vitreoscilla Hemoglobin Expression Alleviate Oxidative Stress In Common Bean Nodules that Reprogramme Global Gene Expression". Plant Biotechnology Reports. 10(6):463-474    
11 2015 Formey, D., Iñiguez-Rabago, L.P., Pelaez, P., Li, Y.F., Sunkar, R., Sánchez, F., Reyes, J.L. and Hernández, G. (2015). "Genome-wide Identification Of The Phaseolus vulgaris sRNAome Using Small RNA and Degradome Sequencing". BMC Genomics. 16:-423   PUBMED: #26059339
12 2015 Hael-Conrad, V., Abou-Mansour, E., Díaz-Ricci, J.C., Metraux, J.P. and Serrano, M. (2015). "The Novel Elicitor Ases Triggers A Defense Response against Botrytis Cinerea In Arabidopsis thaliana". Plant Sci. 241:120-127   PUBMED: #26706064
13 2015 Iñiguez-Rabago, L.P., Nova-Franco, B. and Hernández, G. (2015). "Novel Players In the AP2-miR172 Regulatory Network for Common Bean Nodulation". Plant Signaling and Behavior. 10(10)   PUBMED: #26211831
14 2015 Mendoza-Soto, A.B., Naya, L., Leija, A. and Hernández, G. (2015). "Responses of Symbiotic Nitrogen-fixing Common Bean to Aluminum Toxicity and Delineation of Nodule Responsive microRNAs". Front Plant Sci. 6:-587   PUBMED: #26284103
15 2015 Nova-Franco, B., Iñiguez-Rabago, L.P., Valdés-López, O., Alvarado-Affantranger, X., Leija, A., Fuentes, S.I., Ramirez, M., Paul, S., Reyes, J.L., Girard, L. and Hernández, G. (2015). "The Micro-RNA172c-APETALA2-1 Node As A Key Regulator of the Common Bean-Rhizobium etli Nitrogen Fixation Symbiosis". Plant Physiol. 168(1):273-U502   PUBMED: #25739700
16 2015 Valdés-López, O. and Hernández, G. (2015). "Early Responses In the Root-Rhizobia Interaction". Plant Microbe Interactions. 75:137-147    
17 2014 Garcia-Garcia, J.D., Girard, L., Hernández, G., Saavedra, E., Pardo, J.P., Rodríguez-Zavala, J.S., Encalada, R., Reyes-Prieto, A., Mendoza-Cozatl, D.G. and Moreno-Sánchez, R. (2014). "Zn-bis-glutathionate Is the Best Co-substrate of the Monomeric Phytochelatin Synthase from the Photosynthetic Heavy Metal-hyperaccumulator Euglena Gracilis". Metallomics. 6(3):604-616   PUBMED: #24464102
18 2014 O'Rourke, J.A., Iñiguez-Rabago, L.P., Fu, F.L., Bucciarelli, B., Miller, S.S., Jackson, S.A., McClean, P.E., Li, J., Dai, X.B., Zhao, P.X., Hernández, G. and Vance, C.P. (2014). "An RNA-Seq Based Gene Expression Atlas of the Common Bean". BMC Genomics. 15   PUBMED: #25283805
19 2013 Aparicio-Fabre, R., Guillén, G., Loredo, M., Arellano, J., Valdés-López, O., Ramirez, M., Iñiguez-Rabago, L.P., Panzeri, D., Castiglioni, B., Cremonesi, P., Strozzi, F., Stella, A., Girard, L., Sparvoli, F. and Hernández, G. (2013). "Common Bean (Phaseolus vulgaris L.) Pvtify Orchestrates Global Changes in Transcript Profile Response to Jasmonate and Phosphorus Deficiency". Bmc Plant Biology. 13:26   PUBMED: #23402340
20 2013 O'Rourke, J.A., Iñiguez-Rabago, L.P., Bucciarelli, B., Roessler, J., Schmutz, J., McClean, P.E., Jackson, S.A., Hernández, G., Graham, M.A., Stupar, R.M. and Vance, C.P. (2013). "A Re-sequencing Based Assessment of Genomic Heterogeneity and Fast Neutron-induced Deletions in a Common Bean Cultivar". Front Plant Sci. 4:210   PUBMED: #23805147
21 2013 Ramirez, M., Guillén, G., Fuentes, S.I., Iñiguez-Rabago, L.P., Aparicio-Fabre, R., Zamorano-Sánchez, D., Encarnación, S., Panzeri, D., Castiglioni, B., Cremonesi, P., Strozzi, F., Stella, A., Girard, L., Sparvoli, F. and Hernández, G. (2013). "Transcript Profiling of Common Bean Nodules Subjected to Oxidative Stress". Physiol Plant. 149(3):389-407   PUBMED: #23432573
22 2013 Ramirez, M., Flores-Pacheco, G., Reyes, J.L., Álvarez, A.L., Drevon, J.J., Girard, L. and Hernández, G. (2013). "Two Common Bean Genotypes With Contrasting Response To Phosphorus Deficiency Show Variations In the microRNA 399-mediated PvPHO2 Regulation within the PvPHR1 Signaling Pathway". International Journal of Molecular Sciences. 14(4):8328-8344   PUBMED: #23591845
23 2012 García-García, J.D., Olin-Sandoval, V., Saavedra, E., Girard, L., Hernández, G. and Moreno-Sánchez, R. (2012). "Sulfate Uptake In Photosynthetic Euglena Gracilis. Mechanisms of Regulation and Contribution to Cysteine Homeostasis". Bba-gen Subjects. 1820(10):1567-1575   PUBMED: #22609877
24 2012 Manoj-Kumar, A, Reddy, P.M., Manjulatha, M. and Blanco, L. (2012). "Polysaccharide-free Nucleic Acids and Proteins of Abelmoschus Esculentus for Versatile Molecular Studies". Mol Biol+. 46(4):535-541   PUBMED: #23113348
25 2012 Mendoza-Soto, A.B., Sánchez, F. and Hernández, G. (2012). "MicroRNAs As Regulators in Plant Metal Toxicity Response". Front Plant Sci. 3:105   PUBMED: #22661980
26 2012 Silvente, S., Sobolev, A.P. and Lara-Flores, M. (2012). "Metabolite Adjustments in Drought Tolerant and Sensitive Soybean Genotypes in Response to Water Stress". Plos One. 7(6):e38554   PUBMED: #22685583
27 2011 Islas-Flores, T., Guillen, G., Alvarado-Affantranger, X., Lara-Flores, M., Sáchez, F. and Villanueva, M.A. (2011). "PvRACK1 Loss-of-function Impairs Cell Expansion And Morphogenesis in Phaseolus vulgaris L. Root Nodules". Mol Plant Microbe Interactions. 24(7):819-826   PUBMED: #21425924
28 2011 Nava, E., Dávila-Martínez, Y., Arellano, J., Ortíz, A., Alvarez, L., Marquina, S. and Villarreal, M.L. (2011). "Genetic Transformation of Solanum Chrysotrichum By Agrobacterium tumefaciens and the Production of Antifungal Saponins". In vitro Cell Dev-pl. 47(6):650-657    
29 2010 Ortiz, A., Cardoso-Taketa, A., Monroy, M.R., Arellano, J., Hernández, G. and Villarreal, M.L. (2010). "Transformed Cell Suspension Culture Of Galphimia Glauca Producing Sedative NoR-friedelanes". Planta Med. 76(4):386-392   PUBMED: #19809970
30 2010 Sreevidya, V.S., Hernández-Oane, R.J., Gyaneshwar, P., Lara-Flores, M., Ladha, J.K. and Reddy, P.M. (2010). "Changes in Auxin Distribution Patterns during Lateral Root Development in Rice". Plant Sci. 178(6):531-538    
31 2010 Valdés-López, O., Yang, S.S., Aparicio-Fabre, R., Graham, P.H., Reyes, J.L., Vance, C.P. and Hernández, G. (2010). "MicroRNA Expression Profile In Common Bean (Phaseolus vulgaris) Under Nutrient Deficiency Stresses and Manganese Toxicity". New Phytologist. 187(3):1178-1183   PUBMED: #20553393
32 2010 Yang, S.S., Valdés-López, O., Xu, W.W., Bucciarelli, B., Gronwald, J.W., Hernández, G. and Vance, C.P. (2010). "Transcript Profiling Of Common Bean (Phaseolus vulgaris L.) Using the Genechip (r) Soybean Genome Array: Optimizing Analysis by Masking Biased Probes". Bmc Plant Biology. 10   PUBMED: #20459672
33 2009 Arellano, J., Fuentes, S.I., Castillo-España, P. and Hernández, G. (2009). "Regeneration of Different Cultivars of Common Bean (Phaseolus vulgaris L.) via Indirect Organogenesis.". Plant Cell Tiss Org. 96(1):11-18    
34 2009 Hernández, G., Valdés-López, O., Ramirez, M., Goffard, N., Weiller, G., Aparicio-Fabre, R., Fuentes, S.I., Erban, A., Kopka, J., Udvardi, M.K. and Vance, C.P. (2009). "Global Changes in the Transcript and Metabolic Profiles during Symbiotic Nitrogen Fixation in Phosphorus-stressed Common Bean Plants". Plant Physiol. 151(3):1221-1238   PUBMED: #19755543
35 2008 Blanco, L., Reddy, P.M., Silvente, S., Bucciarelli, B, Khandual, B, Alvarado-Affantranger, X, Sánchez, F., Miller, S, Vance, C and Lara-Flores, M. (2008). "Molecular Cloning, Characterization And Regulation of Two Different NADH-glutamate Synthase cDNAs in Bean Nodules". Plant Cell Environ. 31(4):454-472   PUBMED: #18182018
36 2008 Silvente, S., Reddy, P.M., Khandual, S., Blanco, L., Alvarado-Affantranger, X., Sánchez, F. and Lara-Flores, M. (2008). "Evidence For Sugar Signalling in the Regulation of Asparagine Synthetase Gene Expressed in Phaseolus vulgaris Roots and Nodules". J Exp Bot. 59(6):1279-1294   PUBMED: #18407964
37 2008 Suárez, R., Wong-Villarreal, A., Ramirez, M., Barraza, A., Orozco, M.D., Cevallos, M.A., Lara-Flores, M., Hernández, G. and Iturriaga, G. (2008). "Improvement Of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-phosphate Synthase in Rhizobia". Mol Plant Microbe Interactions. 21(7):958-966   PUBMED: #18533836
38 2008 Valdés-López, O., Arenas-Huertero, C., Ramirez, M., Girard, L., Sánchez, F., Vance, C.P., Reyes, J.L. and Hernández, G. (2008). "Essential Role Of Myb Transcription Factor: PvPHR1 And microRNA: PvmiR399 in Phosphorus-deficiency Signalling in Common Bean Roots". Plant Cell Environ. 31(12):1834-1843   PUBMED: #18771575
39 2008 Valdés-López, O. and Hernández, G. (2008). "Transcriptional Regulation and Signaling in Phosphorus Starvation: What about Legumes?". J. Integr. Plant Biol.. 50(10):1213-1222   PUBMED: #19017108
40 2007 Guo, X.W., Castillo-Ramírez, S., González, V., Bustos, P., Fernández, J.L., Santamaria, R.I., Arellano, J., Cevallos, M.A. and Dávila, G. (2007). "Rapid Evolutionary Change of Common Bean (Phaseolus vulgaris L) Plastome, and the Genomic Diversification of Legume Chloroplasts". BMC Genomics. 8   PUBMED: #17623083
41 2007 Hernández, G., Ramirez, M., Valdés-López, O., Tesfaye, M., Graham, M.A., Czechowski, T., Schlereth, A., Wandrey, M., Erban, A., Cheung, F., Wu, H.C., Lara-Flores, M., Town, C.D., Kopka, J., Udvardi, M.K. and Vance, C.P. (2007). "Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses". Plant Physiol. 144(2):752-767   PUBMED: #17449651
42 2007 Shishkova, S., García-Mendoza, E., Castillo-Díaz, V., Moreno, N.E., Arellano, J. and Dubrovsky, J.G. (2007). "Regeneration of Roots from Callus Reveals Stability of the Developmental Program for Determinate Root Growth in Sonoran Desert Cactaceae". Plant Cell Rep. 26(5):547-557   PUBMED: #17136545
43 2006 Graham, M.A., Ramirez, M., Valdés-López, O., Lara-Flores, M., Tesfaye, M., Vance, C.P. and Hernández, G. (2006). "Identification Of Candidate Phosphorus Stress Induced Genes In Phaseolus vulgaris through Clustering Analysis across Several Plant Species". Funct Plant Biol. 33(8):789-797    
44 2006 Sreevidya, V.S., Rao, C.S., Sullia, S.B., Ladha, J.K. and Reddy, P.M. (2006). "Metabolic Engineering Of Rice with Soybean Isoflavone Synthase for Promoting Nodulation Gene Expression in Rhizobia". J Exp Bot. 57(9):1957-1969   PUBMED: #16690627
45 2005 Ramirez, M., Graham, M.A., Blanco, L., Silvente, S., Medrano-Soto, A., Blair, M.W., Hernández, G., Vance, C.P. and Lara-Flores, M. (2005). "Sequencing and Analysis of Common Bean ESTs. Building a Foundation for Functional Genomics". Plant Physiol. 137(4):1211-1227   PUBMED: #15824284
46 2005 Sreevidya, V.S., Hernandez-Oane, R.J., So, R.B., Sullia, S.B., Stacey, G., Ladha, J.K. and Reddy, P.M. (2005). "Expression of the Legume Symbiotic Lectin Genes psl and gs52 Promotes Rhizobial Colonization of Roots in Rice". Plant Sci. 169(4):726-736    
47 2003 Broughton, W.J., Hernández, G., Blair, M., Beebe, S., Gepts, P. and Vanderleyden, J. (2003). "Beans (Phaseolus Spp.) - Model Food Legumes". Plant Soil. 252(1):55-128    
48 2003 Córdoba, E., Shishkova, S., Vance, C.P. and Hernández, G. (2003). "Antisense Inhibition of NADH Glutamate Synthase Impairs Carbon/nitrogen Assimilation in Nodules of Alfalfa (Medicago sativa L.)". Plant J. 33(6):1037-1049   PUBMED: #12631328
49 2003 Guo, X.W., Flores, M., Mavingui, P., Fuentes, S.I., Hernández, G., Dávila, G. and Palacios, R. (2003). "Natural Genomic Design In Sinorhizobium meliloti Novel Genomic Architectures". Genome Res. 13(8):1810-1817   PUBMED: #12902376
50 2003 Silvente, S., Camas, A. and Lara-Flores, M. (2003). "Heterogeneity Of Sucrose Synthase Genes in Bean (Phaseolus vulgaris L.): Evidence for a Nodule-enhanced Sucrose Synthase Gene". J Exp Bot. 54(383):749-755   PUBMED: #12554718
51 2003 Silvente, S., Camas, A. and Lara-Flores, M. (2003). "Molecular Cloning of the cDNA Encoding Aspartate Aminotransferase from Bean Root Nodules and Determination of Its Role in Nodule Nitrogen Metabolism". J Exp Bot. 54(387):1545-1551   PUBMED: #12730270
52 2003 Suárez, R., Márquez, J., Shishkova, S. and Hernández, G. (2003). "Overexpression of Alfalfa Cytosolic Glutamine Synthetase in Nodules and Flowers of Transgenic Lotus japonicus Plants". Physiol Plant. 117(3):326-336   PUBMED: #12654032
53 2003 Uhde-Stone, C., Zinn, K.E., Ramirez, M., Li, A.G., Vance, C.P. and Allan, D.L. (2003). "Nylon Filter Arrays Reveal Differential Gene Expression in Proteoid Roots of White Lupin in Response to Phosphorus Deficiency". Plant Physiol. 131(3):1064-1079   PUBMED: #12644659
54 2002 Camas, A., Cárdenas, L., Quinto, C. and Lara-Flores, M. (2002). "Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation". Mol Plant Microbe Interactions. 15(5):428-436   PUBMED: #12036273
55 2002 Castillo, P., Zamilpa, A., Marquez, J., Hernández, G., Lara-Flores, M. and Alvarez, L. (2002). "Comparative Study of Differentiation Levels and Valepotriate Content of in vitro Cultures and Regenerated and Wild Plants of Valeriana edulis ssp. procera". J Nat Prod. 65(4):573-575   PUBMED: #11975504
56 2002 Lira-Ruan, V., Ross, E.J.H., Sarath, G., Klucas, R.V. and Arredondo-Peter, R. (2002). "Mapping And Analysis of a Hemoglobin Gene Family from Oryza sativa". Plant Physiol Biochem. 40(3):199-202    
57 2002 Mavingui, P., Flores, M., Guo, X.W., Dávila, G., Perret, X., Broughton, W.J. and Palacios, R. (2002). "Dynamics Of Genome Architecture In Rhizobium sp. Strain NGR234". J Bacteriol. 184(1):171-176   PUBMED: #11741857
58 2002 Silvente, S., Blanco, L., Camas, A., Ortega, J.L., Ramirez, M. and Lara-Flores, M. (2002). "Rhizobium etli Mutant Modulates Carbon And Nitrogen Metabolism In Phaseolus vulgaris Nodules". Mol Plant Microbe Interactions. 15(7):728-733   PUBMED: #12118889
59 2001 Aréchaga-Ocampo, E., Saenz-Rivera, J., Sarath, G., Klucas, R.V. and Arredondo-Peter, R. (2001). "Cloning and Expression Analysis Of Hemoglobin Genes From Maize (Zea mays ssp. mays) and Teosinte (Zea mays ssp. parviglumis)". Bba-gene Struct Express. 1522(1):1-8   PUBMED: #11718894
60 2001 Fuentes, S.I., Allen, D.J., Ortiz-Lopez, A. and Hernández, G. (2001). "Over-expression of Cytosolic Glutamine Synthetase Increases Photosynthesis and Growth at Low Nitrogen Concentrations". J Exp Bot. 52(358):1071-1081   PUBMED: #11432923
61 2001 Lira-Ruan, V., Sarath, G., Klucas, R.V. and Arredondo-Peter, R. (2001). "Synthesis Of Hemoglobins in Rice (Oryza sativa Var. Jackson) Plants Growing in Normal and Stress Conditions". Plant Sci. 161(2):279-287   PUBMED: #11448759
62 2001 Ross, E.J.H., Shearman, L., Mathiesen, M., Zhou, Y.J., Arredondo-Peter, R., Sarath, G. and Klucas, R.V. (2001). "Nonsymbiotic Hemoglobins in Rice are Synthesized during Germination and in Differentiating Cell Types". Protoplasma. 218(3-4):125-133   PUBMED: #11770429
63 2001 Shishkova, S., Arellano, J., Vance, C.P. and Hernández, G. (2001). "Transgenic Tobacco Plants That Overexpress Alfalfa NADH-glutamate Synthase have Higher Carbon and Nitrogen Content". J Exp Bot. 52(364):2079-2087   PUBMED: #11604446
64 2000 Arredondo-Peter, R., Ramírez, Y.M., Sarath, G. and Klucas, R.V. (2000). "Sequence Analysis Of an Ancient Hemoglobin cDNA Isolated from the Moss Physcomitrella patens". Plant Physiol. 122:1457    
65 2000 Castillo, P., Marquez, J., Rubluo, A., Hernández, G. and Lara-Flores, M. (2000). "Plant Regeneration From Callus and Suspension Cultures of Valeriana edulis ssp. procera via Simultaneous Organogenesis and Somatic Embryogenesis". Plant Sci. 151(2):115-119   PUBMED: #10808067
66 2000 Flores, M., Mavingui, P., Perret, X., Broughton, W.J., Romero, D., Hernández, G., Dávila, G. and Palacios, R. (2000). "Prediction, Identification, And Artificial Selection Of DNA Rearrangements in Rhizobium: toward a Natural Genomic Design". P Nat Acad Sci USA. 97(16):9138-9143   PUBMED: #10922070
67 2000 Hargrove, M.S., Brucker, E.A., Stec, B., Sarath, G., Arredondo-Peter, R., Klucas, R.V., Olson, J.S. and Phillips, G.N. (2000). "Crystal Structure of a Nonsymbiotic Plant Hemoglobin". Structure Fold Des. 8(9):1005-1014   PUBMED: #10986467
68 2000 Lira-Ruan, K., Sarath, G., Klucas, R.V. and Arredondo-Peter, R. (2000). "Characterization Of Leghemoglobin from a Mimosoid Legume, Leucaena Esculenta, Root Nodules". Brazilian Journal of Plant Physiology. 12(1):37-44    
69 2000 Luan, P., Aréchaga-Ocampo, E., Sarath, G., Arredondo-Peter, R. and Klucas, R.V. (2000). "Analysis Of a Ferric Leghemoglobin Reductase from Cowpea (Vigna unguiculata) Root Nodules". Plant Sci. 154(2):161-170   PUBMED: #10729615
70 2000 Schoenbeck, M.A., Temple, S.J., Trepp, G.B., Blumenthal, J.M., Samac, D.A., Gantt, J.S., Hernández, G. and Vance, C.P. (2000). "Decreased Nadh Glutamate Synthase Activity in Nodules and Flowers of Alfalfa (Medicago sativa L.) Transformed with an Antisense Glutamate Synthase Transgene". J Exp Bot. 51(342):29-39   PUBMED: #10938793
71 1999 Castillo, M., Flores, M., Mavingui, P., Martínez-Romero, E., Palacios, R. and Hernández, G. (1999). "Increase in Alfalfa Nodulation, Nitrogen Fixation, And Plant Growth by Specific DNA Amplification in Sinorhizobium meliloti". Appl Environ Microbiol. 65(6):2716-2722   PUBMED: #10347066
72 1999 Ramirez, M., Valderrama, B., Arredondo-Peter, R., Soberón, M., Mora, J. and Hernández, G. (1999). "Rhizobium etli Genetically Engineered For The Heterologous Expression of Vitreoscilla sp. Hemoglobin: Effects on Free-living and Symbiosis". Mol Plant Microbe Interactions. 12(11):1008-1015    
73 1998 Arredondo-Peter, R., Hargrove, M.S., Moran, J.F., Sarath, G. and Klucas, R.V. (1998). "Plant Hemoglobins". Plant Physiol. 118(4):1121-1125   PUBMED: #9847086
74 1998 Flores, M., Mavingui, P., Girard, L., Perret, X., Broughton, W.J., Martínez-Romero, E., Dávila, G. and Palacios, R. (1998). "Three Replicons Of Rhizobium Sp. Strain NGR234 Harbor Symbiotic Gene Sequences". J Bacteriol. 180(22):6052-6053   PUBMED: #9811668
75 1998 Mandon, K., Michel-Reydellet, N., Encarnación, S., Kaminski, P.A., Leija, A., Cevallos, M.A., Elmerich, C. and Mora, J. (1998). "Poly-beta-hydroxybutyrate Turnover In Azorhizobium caulinodans is Required for Growth and Affects nifA Expression". J Bacteriol. 180(19):5070-5076   PUBMED: #9748438
76 1998 Mavingui, P., Laeremans, T., Flores, M., Romero, D., Martínez-Romero, E. and Palacios, R. (1998). "Genes Essential For Nod Factor Production And Nodulation Are Located on a Symbiotic Amplicon (AMPRtrCFN299pc60) in Rhizobium tropici". J Bacteriol. 180(11):2866-2874   PUBMED: #9603874
77 1998 Mendoza, A., Valderrama, B., Leija, A. and Mora, J. (1998). "Nifa-dependent Expression Of Glutamate Dehydrogenase in Rhizobium etli Modifies Nitrogen Partitioning during Symbiosis". Mol Plant Microbe Interactions. 11(2):83-90   PUBMED: #9450332
78 1997 Mavingui, P., Flores, M., Romero, D., Martínez-Romero, E. and Palacios, R. (1997). "Generation Of Rhizobium Strains with Improved Symbiotic Properties by Random DNA Amplification (RDA)". Nat Biotechnol. 15(6):564-569   PUBMED: #9181580
79 1996 Arellano, J., Vázquez, F., Villegas, T. and Hernández, G. (1996). "Establishment of Transformed Root Cultures of Perezia cuernavacana Producing the Sesquiterpene Quinone Perezone". Plant Cell Rep. 15(7):455-458   PUBMED: #24178452
80 1996 Cevallos, M.A., Encarnación, S., Leija, A., Mora, Y. and Mora, J. (1996). "Genetic And Physiological Characterization of a Rhizobium etli Mutant Strain Unable to Synthesize Poly-beta-hydroxybutyrate". J Bacteriol. 178(6):1646-1654   PUBMED: #8626293
81 1995 Hernández, G., Ramirez, M., Suárez, R. and Fuentes, S.I. (1995). "Root Exuded Nod-gene Inducing Signals Limit The Nodulation Capacity of Different Alfalfa Varieties with Rhizobium meliloti". Plant Cell Rep. 14(10):626-629   PUBMED: #24194309
82 1995 Mendoza, A., Leija, A., Martínez-Romero, E., Hernández, G. and Mora, J. (1995). "The Enhancement of Ammonium Assimilation in Rhizobium etli Prevents Nodulation of Phaseolus vulgaris". Mol Plant Microbe Interactions. 8(4):584-592   PUBMED: #8589413
83 1993 Fuentes, S.I., Suárez, R., Villegas, T., Castro Acero, L. and Hernández, G. (1993). "Embryogenic Response Of Mexican Alfalfa (Medicago-sativa) Varieties". Plant Cell Tiss Org. 34(3):299-302    
84 1991 Padilla, J.E., Miranda-Ríos, J. and Sánchez, F. (1991). "Nodulin Regulation in Common Bean Nodules Induced by Bacterial Mutants". Mol Plant Microbe Interactions. 4(5):433-439    
85 1990 Soberón, M., Membrillo-Hernández, J., Aguilar, G.R. and Sánchez, F. (1990). "Isolation of Rhizobium phaseoli Tn5-induced Mutants With Altered Expression of Cytochrome Terminal Oxidase o and Oxidase aa3". J Bacteriol. 172(3):1676-1680   PUBMED: #2155209
86 1989 Folch, J., Antaramián, A., Rodríguez, L., Bravo, A., Brunner, A. and González, A. (1989). "Isolation And Characterization Of a saccharomyces cerevisiae Mutant with Impaired Glutamate Synthase Activity". J Bacteriol. 171(12):6776-6781   PUBMED: #2687252
87 1989 Soberón, M., Olamendi, J., Rodríguez, L. and González, A. (1989). "Role Of Glutamine Aminotransferase In Glutamine Catabolism by saccharomyces cerevisiae under Microaerophilic Conditions". J. Gen. Microbiol.. 135:2693-2697   PUBMED: #2698914
88 1989 Soberón, M., William, H.D., Poole, R.K. and Escamilla, E. (1989). "Isolation Of A Rhizobium phaseoli Cytochrome Mutant with Enhanced Respiration and Symbiotic Nitrogen Fixation". J Bacteriol. 171(1):465-472   PUBMED: #2644201
89 1988 Bravo, A. and Mora, J. (1988). "Ammonium Assimilation In Rhizobium phaseoli By the Glutamine Synthetase-glutamate Synthase Pathway". J Bacteriol. 170(2):980-984   PUBMED: #2892829
90 1988 Bravo, A., Becerril, B. and Mora, J. (1988). "Introduction Of The Escherichia coli Gdha Gene into Rhizobium phaseoli: Effect on Nitrogen Fixation". J Bacteriol. 170(2):985-988   PUBMED: #2892830
91 1988 Flores, M., González, V., Pardo, M.A., Leija, A., Martínez-Romero, E., Romero, D., Piñero, D., Dávila, G. and Palacios, R. (1988). "Genomic Instability In Rhizobium phaseoli". J Bacteriol. 170(3):1191-1196   PUBMED: #3343217
92 1987 Campos, F., Padilla, J.E., Vázquez, M., Ortega, J.L., Enríquez, C. and Sánchez, F. (1987). "Expression Of Nodule-Specific Genes In Phaseolus vulgaris L.". Plant Mol Biol. 9(6):521-532   PUBMED: #24277190
93 1987 González, A., Rodríguez, L., Folch, J., Soberón, M. and Olivera, H. (1987). "Coordinated Regulation Of Ammonium Assimilation And Carbon Catabolism by Glyoxylate in saccharomyces cerevisiae". J. Gen. Microbiol.. 133:2497-2501   PUBMED: #2896226
94 1987 Lomnitz, A., Calderón, J., Hernández, G. and Mora, J. (1987). "Functional-analysis of Ammonium Assimilation Enzymes in Neurospora-crassa". J. Gen. Microbiol.. 133:2333-2340    
95 1987 Martínez-Romero, E., Palacios, R. and Sánchez, F. (1987). "Nitrogen-fixing Nodules Induced By Agrobacterium-tumefaciens Harboring Rhizobium-phaseoli Plasmids". J Bacteriol. 169(6):2828-2834   PUBMED: #3584072
96 1987 Mora, Y., Hernández, G. and Mora, J. (1987). "Regulation Of Carbon and Nitrogen Flow by Glutamate Synthase in Neurospora crassa". J. Gen. Microbiol.. 133(7):1667-1674   PUBMED: #2959749
97 1987 Padilla, J.E., Campos, F., Conde, V., Lara-Flores, M. and Sánchez, F. (1987). "Nodule-Specific Glutamine-synthetase Is Expressed before The Onset Of Nitrogen-fixation in Phaseolus-vulgaris L". Plant Mol Biol. 9(1):65-74   PUBMED: #24276799
98 1987 Sánchez, F., Campos, F., Padilla, J.E., Bonneville, J.M., Enríquez, C. and Caput, D. (1987). "Purification, Cdna Cloning, And Developmental Expression of the Nodule-Specific Uricase from Phaseolus vulgaris L.". Plant Physiol. 84(4):1143-1147   PUBMED: #16665575
99 1987 Servín-González, L., Ortíz, M., González, A. and Bastarrachea, F. (1987). "Glna Mutations Conferring Resistance to Methylammonium in Escherichia coli K12". J. Gen. Microbiol.. 133(6):1631-1639   PUBMED: #2889793
100 1987 Soberón, M. and González, A. (1987). "Glutamine Degradation through The Omega-amidase Pathway In saccharomyces cerevisiae". J. Gen. Microbiol.. 133(1):9-14   PUBMED: #3309146
101 1987 Soberón, M. and González, A. (1987). "Physiological Role Of Glutaminase Activity In saccharomyces cerevisiae". J. Gen. Microbiol.. 133(1):1-8   PUBMED: #3309138
102 1986 Hernández, G. and Mora, J. (1986). "Glutamine Synthesis Regulates Sucrose Catabolism In Neurospora crassa". J. Gen. Microbiol.. 132:3315-3323    
103 1986 Hernández, G., Mora, Y. and Mora, J. (1986). "Regulation Of Glutamine Synthesis by Glycine and Serine in Neurospora crassa". J Bacteriol. 165(1):133-138   PUBMED: #2867084
104 1986 Leija, A., Ruíz-Herrera, J. and Mora, J. (1986). "Effect Of L-amino Acids on Mucor rouxii Dimorphism". J Bacteriol. 168(2):843-850   PUBMED: #3096961
105 1986 Ortega, J.L., Campos, F., Sánchez, F. and Lara-Flores, M. (1986). "Expression Of 2 Different Glutamine Synthetase Polypeptides during Root Development In Phaseolus vulgaris L.". Plant Physiol. 80(4):1051-1054   PUBMED: #16664719
106 1985 González, A., Dávila, G. and Calva, E. (1985). "Cloning Of A DNA Sequence that Complements Glutamine Auxotrophy in saccharomyces cerevisiae". Gene. 36(1-2):123-129   PUBMED: #2866146
107 1985 González, A., Rodríguez, L., Olivera, H. and Soberón, M. (1985). "Nadp+-dependent Glutamate Dehydrogenase Activity Is Impaired in Mutants of saccharomyces cerevisiae that Lack Aconitase". J. Gen. Microbiol.. 131(7):2565-2571   PUBMED: #2866224
108 1984 Lara-Flores, M., Porta, H., Padilla, J.E., Folch, J. and Sánchez, F. (1984). "Heterogeneity Of Glutamine Synthetase Polypeptides in Phaseolus vulgaris L.". Plant Physiol. 76(4):1019-1023   PUBMED: #16663942
109 1983 González, A., Tenorio, M., Vaca, G. and Mora, J. (1983). "Neurospora crassa Mutant Impaired In Glutamine Regulation". J Bacteriol. 155(1):1-7   PUBMED: #6134713
110 1983 Hernández, G., Sánchez-Pescador, R., Palacios, R. and Mora, J. (1983). "Nitrogen Source Regulates Glutamate Dehydrogenase Nadp Synthesis in Neurospora crassa". J Bacteriol. 154(1):524-528   PUBMED: #6300039
111 1983 Lara-Flores, M., Cullimore, J.V., Lea, P.J., Miflin, B.J., Johnston, A.W.B. and Lamb, J.W. (1983). "Appearance of A Novel Form of Plant Glutamine Synthetase during Nodule Development in Phaseolus vulgaris L.". Planta. 157(3):254-258   PUBMED: #24264155
112 1982 Lara-Flores, M., Blanco, L., Campomanes, M., Calva, E., Palacios, R. and Mora, J. (1982). "Physiology Of Ammonium Assimilation in Neurospora crassa". J Bacteriol. 150(1):105-112   PUBMED: #6120927

Programa de Genómica Funcional de Eucariotes

Responsable del Programa: Georgina Hernández D, Ph.D.

Este programa de investigación estudia a las PLANTAS, cómo modelo representativo de los sistemas eucariotes, analizando interacciones entre los vegetales y los microorganismos. Incluye cuatro grupos de investigación liderados por los investigadores: Dra. Georgina Hernández, Dr. Mario Serrano, Dr. Alexandre Tromas y Dr. Damien Formey.

Tres líneas de investigación del programa estudian la simbiosis leguminosa-rhizobia. Plantas de la familia de las leguminosas han desarrollado la capacidad de crecer en suelos privados de nitrógeno mediante la interacción con bacterias del suelo conocidas como rhizobia. Los 2 socios han co-evolucionado hacia una interacción muy compleja y positiva para ambos, en la que se lleva a cabo la fijación simbiótica de nitrógeno para capturar el nitrógeno atmosférico directamente y así mantener el crecimiento vegetal.

1. Grupo de Georgina Hernández. Su línea de investigación es la genómica funcional de frijol (Phaseolus vulgaris), la leguminosa más importante para el consumo humano, con énfasis en la transcriptómica tanto de mRNAs codificantes como de RNAs pequeños no-codificantes identificados como reguladores escenciales. El objetivo principal es describir nuevos reguladores globales de la simbiosis frijol-rhizobia, tanto reguladores transcripcionales (factores de transcripción) como post-trancripcionales (microRNAs). Además se explora la regulación post-transcripcional por el “Splicing Alternativo” en la simbiosis frijol-rhizobia.   

2. Grupo de Damien Formey. Su línea de investigación es la caracterización y evolución de los RNA pequeños involucrados en las interacciones planta-microorganismo. Utilizando el modelo de interacción Phaseolus vulgaris / Rhizobium, el proyecto se enfoca en los microRNA y phasiRNA involucrados en la regulación de la fijación simbiótica de nitrógeno, desde el intercambio de factores difusibles hasta la senescencia de los órganos simbióticos llamados nódulos. Varios de estos RNAs pequeños son específicos del frijol común y proveen nuevas percepciones de la co-evolución entre las bacterias y su hospedero.

3. Grupo de Alexandre Tromas. Su línea de investigación es la regulación del citoesqueleto durante la fijación simbiótica de nitrógeno. Se ha establecido que el acomodo de los microsimbiontes, como rhizobia, dentro de la célula vegetal es un proceso activo que requiere del adecuado re-arreglo del citoesqueleto. Las Rho-GTPasas (denominadas ROP, en las plantas), consideradas como “switches moleculares”, activan el re-arreglo de la actina, uno de los principales componentes del citoesqueleto. El objetivo principal es entender cuáles complejos  re-arreglos de complejos del citoesqueleto están involucrados en el acomodo de las bacterias simbióticas y entender cuando y donde se re-localizan y activan estos en los pelos radicales de la leguminosa modelo Lotus japonicus.

Otra línea de investigación del programa estudia la inmunidad innata vegetal.

4.      Grupo de Mario Serrano. Su línea de investigación es la caracterización de la inmunidad innata al hongo necrótrofo Botrytis cinerea (moho gris), que se considera el segundo hongo fitopatógeno más dañino para la agricultura. Las primeras interacciones entre las plantas y los hongos tienen lugar en la epidermis, donde la planta no solo tiene la primera barrera física, que incluye la cutícula en los órganos aéreos, sino es donde los organismos se reconocen mutuamente y potencialmente la inmunidad innata vegetal es inducida. El objetivo del proyecto es identificar y caracterizar los elementos moleculares que vinculan la degradación de la cutícula y la inducción de la inmunidad innata de las plantas, utilizamos el modelo de interacción Arabidopsis thaliana-Botrytis cinerea y las herramientas de genética tradicional y genómica química.

Alternative splicing in common bean

The post-genomic era lead to the discovery of alternative splicing in eukaryotes (plants and animals). Splicing of introns from a pre-mRNAs transcribed from a single eukaryotic gene can be regulated by different factors and so generate alternative spliced transcripts or isoforms. The analysis of the great complexity at the transcriptional level generated by alternative splicing in legumes has only begun. Based in our common bean transcriptome data we are analyzing the alternative splicing in this legume (collaboration with S. Jackson, U. Georgia).

MicroRNAs as global regulators of common bean symbiosis and of the response to abiotic stress

Micro-RNAs (miRNAs), a class of small non-coding RNAs 21-24 nt long, are essential post-transcriptional regulators for plant development and response to stress; their characterization and function in legumes is yet scant. We are interested in enhancing the knowledge about miRNAs in common bean, focusing in SNF nodules. Projects in this area include: a) miRNAs expression profiles through RNA-seq and b) defining specific functions of selected miRNAs in nodule development and/or in the response of SNF common bean plants to abiotic stress using reverse genetic approaches.

Symbiotic transcriptome (common bean / rhizobium) of the response to abiotic stress

The effectiveness of the atmospheric N-fixation process occurring in the legume-rhizobia symbiosis (symbiotic nitrogen fixation = SNF) is highly dependent on the adequate gene expression both in the macro- and micro-symbiont. One of our research interests is to perform combined transcriptome analyses of the Rhizobium etli bacteroids and the common bean nodule cells to generate integral knowledge of global gene expression and regulation in this symbiosis. Such project is done in collaboration with the group of L.

Common bean expression atlas: transcription factors

Our group has participated in pioneer research on common bean transcriptome. This work has been done in collaboration with Carroll P. Vance, U. Minnesota – USDA, and other colleagues.