Generation of tools for genome engineering in Rhizobium
Genome Engineering is defined as the in vivo modification of the genome, using homologous or site-specific recombination. In this area, we are taking advantage of our previous experience in selection of genome rearrangements. Moreover, we are implementing several tools for
the generation of programmed deletions (using the Cre-loxP system), “erasable marker” technology (also using the Cre-loxP system), efficient introduction of point mutations (using gene conversion) and efficient introduction of marked mutations (using recombineering or approaches based in the in vivo delivery of double-strand cuts).