Víctor Manuel González Zúñiga

Evolutionary Genomics Researcher

Associate Professor

Academic history

He was born in Poza Rica, Veracruz, and studied Biology at the Universidad Veracruzana. His Diploma thesis work, supervised by Dr. Rafael Palacios, was done in the Centro de Investigación Sobre Fijación de Nitrógeno (CIFN-UNAM). The thesis was about the characterization of repeated DNA sequences and genomic rearrangements in Rhizobium. Soon afterwards he obtained his Master Degree in Biomedical Sciences at CIFN-UNAM, under the guidance of Dr. Esperanza Martínez, with a work related to the conservation of plasmid DNA sequences in R. etli. He spent some years as a researcher in the Instituto Nacional de Salud Pública, contributing to understanding the frequency of mutations conferring fluoroquinolone resistance to Salmonella. His Doctoral studies were done at the Instituto de Biotecnología-UNAM, under the direction of Dr. Enrique Morett and Dr. Xavier Soberón. This work put forward the importance of specific amino acids for the recognition between nifA and the RNA pol-s54 holoenzyme. He came back to CIFN-UNAM as a researcher and directed the group that achieved the first complete genome of an organism, Rhizobium etli, done in México. Currently, he is interested in the bacteria and phage interactions at the community level. His research group uses genomic approaches in the frame of the evolution theory to study the ecology and genetics of bacteria and phage populations. Moreover, he collaborates with distinct research groups in the comparative genomic analysis of pathogenic and biotechnological relevant bacteria.

Links

Scopus

Telephone: (777) 3133881
Email: vgonzal@ccg.unam.mx

Publications

YearPublicationPMID
2017Sigala, J.C., Suárez, B.P., Lara, A.R., Borgne, S.L., Bustos, P., Santamaría, R.I., González, V. and Martinez, A.. (2017). "Genomic and physiological characterization of a laboratory-isolated Acinetobacter Schindleri ACE strain that quickly and efficiently catabolizes acetate". MICROBIOLOGY-SGM. 163(7):1052-1064. [doi:10.1099/mic.0.000488]28671531
2016Perez Carrascal, Olga M., VanInsberghe, David, Juarez, Soledad, Polz, Martin F., Vinuesa, P. and González, V.. (2016). "Population genomics of the symbiotic plasmids of sympatric nitrogen-fixing Rhizobium species associated with Phaseolus vulgaris". Environmental Microbiology. 18(8):2660-2676. [doi:10.1111/1462-2920.13415]27312778
2015Ormeño-Orrillo, E., Servín-Garcidueñas, L.E., Rogel, M.A., González, V., Peralta, H., Mora, J., Martínez, J. and Martínez-Romero, E.. (2015). "Taxonomy of Rhizobia and agrobacteria from the Rhizobiaceae family in light of genomics". Systematic And Applied Microbiology. 38(4):287-291. [doi:10.1016/j.syapm.2014.12.002]25660942
2015Lozano, Luis, Santamaria Gutiérrez, Rosa Isela and Bustos, P. (2015). "Genomic Tools for the Study of Azospirillum and Other Plant Growth-Promoting Rhizobacteria". Handbook for Azospirillum: Technical Issues and Protocols. ():83-97. [doi:10.1007/978-3-319-06542-7_5]
2015Padilla, J.C.A., Bustos, P., Castro-Escarpulli, G., Sánchez-Varela, A., Palma-Martinez, I., Arzate-Barbosa, P., García-Pérez, C.A., López-López, M.d.J., González, V. and Guo, X.. (2015). "Draft genome sequence of Aeromonas caviae strain 429865 INP, isolated from a Mexican patient". Genome Announcements. 3(5):e01240-15-. [doi:10.1128/genomeA.01240-15]26494682
2014Rogel, M.A., Bustos, P., Santamaría, R.I., González, V., Romero, D., Cevallos, M.A., Lozano, L., Castro-Mondragón, J., Martínez, J., Ormeño-Orrillo, E. and Martínez-Romero, E.. (2014). "Genomic basis of symbiovar mimosae in Rhizobium etli". Bmc Genomics. 15(1):575-575. [doi:10.1186/1471-2164-15-575]25005495
2014Alberto Batista-Garcia, Ramon, Sanchez-Reyes, Ayixon, Millan-Pacheco, Cesar, González, V., Juarez, Soledad, Luis Folch-Mallol, Jorge and Pastor, Nina. (2014). "A novel TctA citrate transporter from an activated sludge metagenome: Structural and mechanistic predictions for the TTT family". Proteins-Structure Function And Bioinformatics. 82(9):1756-1764. [doi:10.1002/prot.24529]24493659
2014Santamaria Gutiérrez, Rosa Isela, Bustos Arcos, Virginia Patricia, Lozano Aguirre Beltrán, Luis Fernando, Rodríguez Sánchez, César, Fernandez Vázquez, José Luis, Juárez Ramírez, María Soledad, Kameyama, L., Guarneros, G., Dávila Ramos, José Guillermo and González, V.. (2014). "Narrow-Host-Range Bacteriophages That Infect Rhizobium etli Associate with Distinct Genomic Types". Applied And Environmental Microbiology. 80(2):446-454. [doi:10.1128/AEM.02256-13]24185856
2013Tsai, I.J., Zarowiecki, M., Holroyd, N., Garciarrubio, A., Sánchez-Flores, A., Brooks, K.L., Tracey, A., Bobes, R.J., Fragoso, G., Sciutto, E., Aslett, M., Beasley, H., Bennett, H.M., Cai, J., Camicia, F., Clark, R., Cucher, M., De Silva, N., Day, T.A., Deplazes, P., Estrada, K., Fernández, C., Holland, P.W., Hou, J., Hu, S., Huckvale, T., Hung, S.S., Kamenetzky, L., Keane, J.A., Kiss, F., Koziol, U., Lambert, O., Liu, K., Luo, X., Luo, Y., Macchiaroli, N., Nichol, S., Paps, J., Parkinson, J., Pouchkina-Stantcheva, N., Riddiford, N., Rosenzvit, M., Salinas, G., Wasmuth, J.D., Zamanian, M., Zheng, Y., Garciarrubio, A., Bobes, R.J., Fragoso, G., Sánchez-Flores, A., Estrada, K., Cevallos, M.A., Morett, E., González, V., Portillo, T., Ochoa-Leyva, A., José, M.V., Sciutto, E., Landa, A., Jiménez, L., Valdés, V., Carrero, J.C., Larralde, C., Morales-Montor, J., Limón-Lason, J., Soberón, X., Laclette, J.P., Cai, X., Soberón, X., Olson, P.D., Laclette, J.P., Brehm, K. and Berriman, M.. (2013). "The genomes of four tapeworm species reveal adaptations to parasitism". Nature. 496(7443):57-63. [doi:10.1038/nature12031]23485966
2012Fernández-Sandoval, M.T., Huerta-Beristain, G., Trujillo-Martínez, B., Bustos Arcos, Virginia Patricia, González, V., Bolivar, F., Gosset, G. and Martínez, A.. (2012). "Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium". Applied Microbiology And Biotechnology. 96(5):1291-1300. [doi:10.1007/s00253-012-4177-y]22669633
2012Althabegoiti, M.J., Lozano Aguirre Beltrán, Luis Fernando, Torres-Tejerizo, G., Ormeño Orrillo, Ernesto Aldo, Rogel Hernández, Marco Antonio, González, V. and Martínez-Romero, E.. (2012). "Genome sequence of Rhizobium Grahamii CCGE502, a broad-host-range symbiont with low nodulation competitiveness in Phaseolus vulgaris". Journal Of Bacteriology. 194(23):6651-6652. [doi:10.1128/JB.01785-12]23144400
2012Wisniewski-Dye, Florence, Lozano, Luis, Acosta-Cruz, Erika, Borland, Stephanie, Drogue, Benoit, Prigent-Combaret, Claire, Rouy, Zoe, Barbe, Valerie, Mendoza Herrera, Alberto, González, V. and Mavingui, Patrick. (2012). "Genome sequence of Azospirillum brasilense CBG497 and comparative analyses of Azospirillum core and accessory genomes provide insight into niche adaptation". Genes. 3(4):576-602. [doi:10.3390/genes3040576]24705077
2012Tejerizo, G.T., Lozano, L, González, V., Bustos, P, Romero, D. and Brom, S.. (2012). "Draft genome sequence of the bean-nodulating Sinorhizobium fredii strain GR64". Journal Of Bacteriology. 194(24):6978-6978. [doi:10.1128/JB.01882-12]23209231
2012de la Rosa, J.J.P., Ureostegui, P.V., Martínez, J.A.A., Martínez, C.R., González, V. and Millan, J.V.F.. (2012). "Babesia bigemina: Initial gene identification by Expressed Sequence Tags (EST) analysis of the intraerythrocytic stage". REVISTA MEXICANA DE CIENCIAS PECUARIAS. 3(1):61-87.
2012López Guerrero, Martha Guadalupe, Ormeño Orrillo, Ernesto Aldo, Velázquez, E., Rogel Hernández, Marco Antonio, CCG, González, V., Martínez, J. and Martínez-Romero, E.. (2012). "Rhizobium etli taxonomy revised with novel genomic data and analyses". Systematic And Applied Microbiology. 35(6):353-358. [doi:10.1016/j.syapm.2012.06.009]22858268
2012Mavingui, P., Van, T.V., Wisniewski-Dye, F., Raquin, V., Minard, G., Tran, F.H., Voronin, D., Rouy, Z., Bustos Arcos, Virginia Patricia, Lozano Aguirre Beltrán, Luis Fernando, Barbe, V. and González, V.. (2012). "Whole-genome sequence of Wolbachia strain wALbB, an endosymbiont of tiger mosquito vector Aedes albopictus". Journal Of Bacteriology. 194(7):1840-1840. [doi:10.1128/JB.00036-12]22408242
2011Wisniewski-Dye, F., Borziak, K., Khalsa-Moyers, G., Alexandre, G., Sukharnikov, L.O., Wuichet, K., Hurst, G.B., McDonald, W.H., Robertson, J.S., Barbe, V., Calteau, A., Rouy, Z., Mangenot, S., Prigent-Combaret, C., Normand, P., Boyer, M., Siguier, P., Dessaux, Y., Elmerich, C., Condemine, G., Krishnen, G., Kennedy, I., Paterson, A.H., González, V., Mavingui, P. and Zhulin, I.B.. (2011). "Azospirillum genomes reveal transition of bacteria from aquatic to terrestrial environments". Plos Genetics. 7(12):e1002430-. [doi:10.1371/journal.pgen.1002430]22216014
2010Lozano Aguirre Beltrán, Luis Fernando, Hernández González, Ismael Luis, Bustos Arcos, Virginia Patricia, Santamaria Gutiérrez, Rosa Isela, Souza, V., Young, J.P.W, Dávila Ramos, José Guillermo and González, V.. (2010). "Evolutionary dynamics of insertion sequences in relation to the evolutionary histories of the chromosome and symbiotic plasmid genes of Rhizobium etli populations". Applied And Environmental Microbiology. 76(19):6504-6513. [doi:10.1128/AEM.01001-10]20675442
2010González, V., Acosta, Jose L., Santamaria, Rosa I., Bustos, Patricia, Fernandez, Jose L., Hernandez Gonzalez, Ismael L., Diaz, Rafael, Flores, Margarita, Palacios, Rafael, Mora, J. and Davila, Guillermo. (2010). "Conserved symbiotic plasmid DNA sequences in the multireplicon pangenomic structure of Rhizobium etli". Applied And Environmental Microbiology. 76(5):1604-1614. [doi:10.1128/AEM.02039-09]20048063
2009Garza-Ramos, U., Davila, G., González, V., Alpuche-Aranda, C., Lopez-Collada, V. R., Alcantar-Curiel, D., Newton, O. and Silva-Sanchez, J.. (2009). "The blaSHV-5gene is encoded in a compound transposon duplicated in tandem in Enterobacter Cloacae". Clinical Microbiology And Infection. 15(9):878-880. [doi:10.1111/j.1469-0691.2009.02790.x]19519856
2009Morett, Enrique, Garciarubio, A., Bobes, R. J., Carrero, J. C., Cevallos, M.A., Fragoso, G., Gaytan, P., González, V., Jose, M. V., Jimenez, L., Landa, A., Larralde, C., Morales-Montor, J., Sciutto, E., Soberon, X., de la Torre, P., Valdes, V., Yanez, J. and Laclette, J. P.. (2009). "The Genome Project of Taenia solium". Infection Genetics And Evolution. 9(3):368-369.
2008Crossman, Lisa C., Castillo-Ramirez, S., McAnnula, Craig, Lozano, Luis, Vernikos, Georgios S., Acosta, Jose L., Ghazoui, Zara F., Hernandez-Gonzalez, Ismael, Meakin, Georgina, Walker, Alan W., Hynes, Michael F., Young, J. Peter W., Downie, J. Allan, Romero, D., Johnston, Andrew W. B., Davila, Guillermo, Parkhill, Julian and González, V.. (2008). "A common genomic framework for a diverse assembly of plasmids in the symbiotic nitrogen fixing bacteria". Plos One. 3(7):e2567-. [doi:10.1371/journal.pone.0002567]18596979
2008Garciarrubio, A., Bobes, R. J., Carrero, J. C., Cevallos, M.A., Fragoso, G., González, V., Jose, M. V., Landa, A., Larralde, C., Mendoza, L., Morales-Montor, J., Morett, E., Sciutto, E., Soberon, X. and Laclette, J. P.. (2008). "The Genome Project of Taenia solium". International Journal Of Infectious Diseases. 12():395-395. [doi:10.1016/j.ijid.2008.05.1042]
2008Castillo-Ramirez, S. and González, V.. (2008). "Factors affecting the concordance between orthologous gene trees and species tree in bacteria". Bmc Evolutionary Biology. 8(1):300-300. [doi:10.1186/1471-2148-8-300]18973688
2008Boyer, Mickael, Haurat, Jacqueline, Samain, Sylvie, Segurens, Beatrice, Gavory, Frederick, González, V., Mavingui, Patrick, Rohr, Rene, Bally, Rene and Wisniewski-Dye, Florence. (2008). "Bacteriophage prevalence in the genus Azospirillum and analysis of the first genome sequence of an Azospirillum brasilense integrative phage". Applied And Environmental Microbiology. 74(3):861-874. [doi:10.1128/AEM.02099-07]18065619
2008González, V., Luis Lozano, Castillo-Ramirez, S., Hernández González, Ismael Luis, Bustos Arcos, Virginia Patricia, Santamaria Gutiérrez, Rosa Isela, Fernandez Vázquez, José Luis, CCG and Dávila Ramos, José Guillermo. (2008). "Evolutionary Genomics of the Nitrogen Fixing Symbiotic Bacteria". Molecular Mechanisms of Plant-Microbe Coexistance Soil Biology Series. 15(0):183-198.
2007Guo, Xianwu, Castillo-Ramirez, S., González, V., Bustos, Patricia, Fernandez-Vazquez, Jose Luis, Santamaria, Rosa Isela, Arellano, Jesus, Cevallos, M.A. and Davila, Guillermo. (2007). "Rapid evolutionary change of common bean (Phaseolus vulgaris L) plastome, and the genomic diversification of legume chloroplasts". Bmc Genomics. 8():228-228. [doi:10.1186/1471-2164-8-228]17623083
2007Flores López, Margarita, Morales Reyes, Lucía Guadalupe, Gonzaga Jáuregui, Claudia Gabriela, Domínguez Vidaña, Rocío, Zepeda Mendoza, Cinthya Jeanette, Yáñez Cuna, Jorge Omar, Gutiérrez Arcelus, María, Lemus Vergara, Tzitziki Janik, Valle García, David, Ávila Arcos, María del Carmen, Blanco Melo, Daniel, Medina Ruiz, Sofía, Meza Sosa, Karla Fabiola, Ayala Ortega, Erandi, García Alonso, Delfino, Bustos Arcos, Virginia Patricia, González, V., Girard, L., Tusie-Luna, T., Dávila Ramos, José Guillermo and Palacios de la Lama, Rafael. (2007). "Recurrent DNA inversion rearrangements in the human genome". PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 104(15):6099-6106. [doi:10.1073/pnas.0701631104]17389356
2007Guo, Xianwu, Flores, Margarita, Morales, Lucia, Garcia, Delfino, Bustos, Patricia, González, V., Palacios, Rafael and Davila, Guillermo. (2007). "DNA diversification in two Sinorhizobium species". Journal Of Bacteriology. 189(17):6474-6476. [doi:10.1128/JB.00384-07]17601787
2006Ramirez-Romero, MA, Masulis, I, Cevallos, M.A., González, V. and Davila, G. (2006). "The Rhizobium etli s70 (SigA) factor recognizes a lax consensus promoter". Nucleic Acids Research. 34(5):1470-1480. [doi:10.1093/nar/gkl023]16528104
2006Aguilar-Diaz, H, Bobes, RJ, Carrero, JC, Camacho-Carranza, R, Cervantes, C, Cevallos, M.A., Davila, G, Rodriguez-Dorantes, M, Escobedo, G, Fernandez, JL, Fragoso, G, Gaytan, P, Garciarubio, A, González, V., Gonzalez, L, Jose, MV, Jimenez, L, Laclette, JP, Landa, A, Larralde, C, Morales-Montor, J, Morett, E, Ostoa-Saloma, P, Sciutto, E, Santamaria, RI, Soberon, X, de la Torre, P, Valdes, V and Yanez, J. (2006). "The genome project of Taenia solium". PARASITOLOGY INTERNATIONAL. 55(SUPPL.):127-130. [doi:10.1016/j.parint.2005.11.020]16337432
2006González, V., Santamaria Gutiérrez, Rosa Isela, Bustos Arcos, Virginia Patricia, Hernández González, Ismael Luis, Medrano Soto, Luis Arturo, Moreno Hagelsieb, Gabriel, Janga, Sarath Chandra, Ramírez Romero, Miguel Ángel, Jiménez Jacinto, Verónica, Collado-Vides, J. and Dávila Ramos, José Guillermo. (2006). "The partitioned Rhizobium etli genome: Genetic and metabolic redundancy in seven interacting replicons". PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 103(10):3834-3839. [doi:10.1073/pnas.0508502103]16505379
2006González, V., Santamaría, R.I., Bustos, P., Acosta, J.L., Fernández, J.L., Hernández-González, I., Lozano, L., Castillo-Ramirez, S. and Dávila, G.. (2006). "Genomic diversity of Rhizobium etli: The Complete Genome and its -intraspecific-variation". Revista Latinoamericana De Microbiologia. 48(2):132-134.
2005Flores López, Margarita, Morales Reyes, Lucía Guadalupe, Ávila Casanueva, Agustin Bernardo, González, V., Bustos Arcos, Virginia Patricia, García Alonso, Delfino, Pérez-Tejada Domínguez, Yolanda, Guo, Xianwu, Collado-Vides, J., Pinero, D., Dávila Ramos, José Guillermo, Mora, J. and Palacios de la Lama, Rafael. (2005). "Diversification of DNA sequences in the symbiotic genome of Rhizobium etli". Journal Of Bacteriology. 187(21):7185-7192. [doi:10.1128/JB.187.21.7185-7192.2005]16237002
2005Dávila Ramos, José Guillermo, González, V., Ramírez Romero, Miguel Ángel and Rodríguez Sánchez, Oscar. (2005). "Rhizobium etli genome biology". Nitrogen Fixation: Origins, Applications and Research Progress. 3():133-142.
2004Brom, S., Girard, L., Tun Garrido, Cristina Soleda, García-de los Santos, A., Bustos Arcos, Virginia Patricia, González, V. and Romero, D.. (2004). "Transfer of the symbiotic plasmid of Rhizobium etli CFN42 requires cointegration with p42a, which may be mediated by site-specific recombination". Journal Of Bacteriology. 186(22):7538-7548. [doi:10.1128/JB.186.22.7538-7548.2004]15516565
2003González, V., Bustos Arcos, Virginia Patricia, Ramírez Romero, Miguel Ángel, Medrano Soto, Luis Arturo, Salgado Osorio, Heladia, Hernández González, Ismael Luis, Hernández Celis, Juan Carlos, Quintero Hernández, Verónica, Moreno Hagelsieb, Gabriel, Girard, L., Rodríguez Sánchez, Oscar, Flores López, Margarita, Cevallos, M.A., Collado-Vides, J., Romero, D. and Dávila Ramos, José Guillermo. (2003). "The mosaic structure of the symbiotic plasmid of Rhizobium etli CFN42 and its relation to other symbiotic genome compartments.". Genome Biology. 4(6):R36-. [doi:10.1186/gb-2003-4-6-r36]12801410
2003Tun-Garrido, C, Bustos, P, González, V. and Brom, S.. (2003). "Conjugative transfer of p42a from Rhizobium etli CFN42, which is required for mobilization of the symbiotic plasmid, is regulated by quorum sensing". Journal Of Bacteriology. 185(5):1681-1692. [doi:10.1128/JB.185.5.1681-1692.2003]12591886
1988FLORES, M, González, V., PARDO, MA, Leija, A, Martínez-Romero, E., Romero, D., Piñero, D., DAVILA, G and PALACIOS, R. (1988). "Genomic instability in Rhizobium phaseoli.". Journal Of Bacteriology. 170(3):1191-1196. [doi:10.1128/jb.170.3.1191-1196.1988]3343217
1987FLORES, M, González, V., Brom, S., Martínez-Romero, E., PINERO, D, Romero, D., DAVILA, G and PALACIOS, R. (1987). "Reiterated DNA sequences in Rhizobium and Agrobacterium spp.". Journal Of Bacteriology. 169(12):5782-5788. [doi:10.1128/jb.169.12.5782-5788.1987]3450286
2006Alvarez, R.G., de Mendoza, A.R., Escobedo, D.E., Felix, G.C., Sanchez, I.R., González, V., Dávila Ramos, José Guillermo, Cao, Q., de Jong, P., Fu, Y.X. and Saldana, H.A.B.. (2006). "Growth hormone locus expands and diverges after the separation of New and Old World Monkeys". Gene. 380(1):38-45. [doi:10.1016/j.gene.2006.05.017]16872758
2009Martinez-Salazar, Jaime M., Sandoval-Calderon, Mario, Guo, Xianwu, Castillo-Ramirez, S., Reyes, Alma, Loza, Maria G., Rivera, Javier, Alvarado-Affantranger, Xochitl, Sanchez, Federico, González, V., Davila, Guillermo and Ramirez-Romero, Miguel A.. (2009). "The Rhizobium etli RpoH1 and RpoH2 sigma factors are involved in different stress responses". MICROBIOLOGY-SGM. 155(2):386-397. [doi:10.1099/mic.0.021428-0]19202087
2009Castillo-Ramirez, S., Vazquez-Castellanos, Jorge F., González, V. and Cevallos, M.A.. (2009). "Horizontal gene transfer and diverse functional constrains within a common replication-partitioning system in Alphaproteobacteria: The repABC operon". Bmc Genomics. 10():536-536. [doi:10.1186/1471-2164-10-536]19919719
2011Acosta, Jose L., Eguiarte, L.E., Santamaria Gutiérrez, Rosa Isela, Bustos Arcos, Virginia Patricia, Vinuesa, P., Martínez-Romero, E., Dávila Ramos, José Guillermo and González, V.. (2011). "Genomic lineages of Rhizobium etli revealed by the extent of nucleotide polymorphisms and low recombination". Bmc Evolutionary Biology. 11(1):305-305. [doi:10.1186/1471-2148-11-305]22004448
2017Bustos P, Santamaría RI, Pérez-Carrascal OM, Acosta JL, Lozano L, Juárez S, Martínez-Flores I, Martínez-Romero, E., Cevallos, M.A., Romero, D., Dávila G, Vinuesa, P., Miranda F, Ormeño E and González, V.. (2017). "Complete genome sequences of three Rhizobium gallicum symbionts associated with common bean (Phaseolus vulgaris)". Genome Announcements. 5(11):e00030-17-. [doi:10.1128/genomeA.00030-17]28302777
2017Santamaría RI, Bustos P, Pérez-Carrascal OM, Miranda-Sánchez F, Vinuesa, P., Martínez-Flores I, Juárez S, Lozano L, Martínez-Romero, E., Cevallos, M.A., Romero, D., Dávila G, Ormeño-Orrillo E and González, V.. (2017). "Complete genome sequences of eight Rhizobium symbionts associated with common bean (Phaseolus vulgaris)". Genome Announcements. 5(30):e00645-17-. [doi:10.1128/genomeA.00645-17]28751391