Interactions between Pro- and Eukaryotes Group
Biosynthesis of ornithine-containing lipids, a widespread class of bioactive lipids in eubacterial membranes
Principal investigator: Otto Geiger
Formación de nuevos lípidos de membrana en rizobia bajo condiciones de estrés
Principal investigator: Christian Sohlenkamp
Participants: Otto Geiger, Isabel María López-Lara, Miguel Angel Vences-Guzmán
Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation
Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation
Participants: Otto Geiger, Christian Sohlenkamp, Ana Laura Ramos-Vega, Yadira Dávila-Martínez, Sandra Leticia Beltrán-Escobar
Biosynthesis of choline-derived bacterial cell surface components...
Biosynthesis of choline-derived bacterial cell surface components: an essential requirement for the success of many pathogenic bacteria
Principal investigator: Otto Geiger
Formation, function, and replacement of membrane lipids in rhizobia
Principal investigator: Otto Geiger
Grupo de Interacciones entre Pro- y Eucariotes
Investigador Responsable Otto Geiger, Ph.D.
The research group
By the time this Research Group joined the Nitrogen Fixation Research Center in 1999, the laboratory of Otto Geiger had discovered the phosphatidylcholine synthase pathway, which constitutes a major route for phosphatidylcholine (lecithine) formation in bacteria. This finding started a research line aimed to understand how molecules of the bacterial membrane or cell surface modify defense responses of eukaryotic hosts thus allowing long-lasting or permanent infections which can lead to symbiotic or pathogenic interactions with the host.
Publicaciones
| # | Año | Ficha | Ligas |
| 1 | 2017 | López-Lara, I.M. and Geiger, O. (2017). "Bacterial Lipid Diversity". Bba-mol Cell Biol L. 1862(11):1287-1299 | PUBMED: #27760387 |
| 2 | 2017 | Sandoval-Calderón, M., Guan, Z.Q. and Sohlenkamp, C. (2017). "Knowns and Unknowns of Membrane Lipid Synthesis in Streptomycetes". Biochimie. 141:21-29 | PUBMED: #28522365 |
| 3 | 2017 | Vences-Guzmán, M.Á., Goetting-Minesky, M.P., Guan, Z.Q., Castillo-Ramírez, S., Cordoba-Castro, L.A., López-Lara, I.M., Geiger, O., Sohlenkamp, C. and Fenno, J.C. (2017). "1,2-Diacylglycerol Choline Phosphotransferase Catalyzes the Final Step In the Unique Treponema denticola Phosphatidylcholine Biosynthesis Pathway". Mol Microbiol. 103(5):896-912 | PUBMED: #28009086 |
| 4 | 2016 | Sahonero-Canavesi, D.X., Zavaleta-Pastor, M., Martínez-Aguilar, L., López-Lara, I.M. and Geiger, O. (2016). "Defining Substrate Specificities for Lipase and Phospholipase Candidates". Jove-journal of Visualized Experiments. (117)-e54613 | PUBMED: #27911408 |
| 5 | 2016 | Sohlenkamp, C. and Geiger, O. (2016). "Bacterial Membrane Lipids: Diversity in Structures and Pathways". FEMS Microbiol Rev. 40(1):133-159 | PUBMED: #25862689 |
| 6 | 2016 | Wang, M.X., Carver, J.J., Phelan, V.V., Sánchez, L.M., Garg, N., Peng, Y., Nguyen, D.D., Watrous, J., Kapono, C.A., Luzzatto-Knaan, T., Porto, C., Bouslimani, A., Melnik, A.V., Meehan, M.J., Liu, W.T., Criisemann, M., Boudreau, P.D., Esquenazi, E., Sandoval-Calderón, M., Kersten, R.D., Pace, L.A., Quinn, R.A., Duncan, K.R., Hsu, C.C., Floros, D.J., Gavilan, R.G., Kleigrewe, K., Northen, T., Dutton, R.J., Parrot, D., Carlson, E.E., Aigle, B., Michelsen, C.F., Jelsbak, L., Sohlenkamp, C., Pevzner, P., Edlund, A., McLean, J., Piel, J., Murphy, B.T., Gerwick, L., Liaw, C.C., Yang, Y.L., Humpf, H.U., Maansson, M., Keyzers, R.A., Sims, A.C., Johnson, A.R., Sidebottom, A.M., Sedio, B.E., Klitgaard, A., Larson, C.B., Boya, C.A., Torres-Mendoza, D., González, D.J., Silva, D.B., Marques, L.M., Demarque, D.P., Pociute, E., ONeill, E.C., Helfrich, E.J.N., Granatosky, E.A., Glukhov, E., Ryffel, F., Houson, H., Mohimani, H., Kharbush, J.J., Zeng, Y., Vorholt, J.A., Kurita, K.L., Charusanti, P., McPhail, K.L., Nielsen, K.F., Vuong, L., Elfeki, M., Traxler, M.F., Engene, N., Koyama, N., Vining, O.B., Baric, R., Silva, R.R., Mascuch, S.J., Tomasi, S., Jenkins, S., Macherla, V., Hoffman, T., Agarwal, V., Williams, P.G., Dai, J.Q., Neupane, R., Gurr, J., Rodríguez, A.M.C., Lamsa, A., Zhang, C., Dorrestein, K., Duggan, B.M., Almaliti, J., Allard, P.M., Phapale, P., Nothias, L.F., Alexandrovr, T., Litaudon, M., Wolfender, J.L., Kyle, J.E., Metz, T.O., Peryea, T., Nguyen, D.T., VanLeer, D., Shinn, P., Jadhav, A., Muller, R., Waters, K.M., Shi, W.Y., Liu, X.T., Zhang, L.X., Knight, R., Jensen, P.R., Palsson, B.O., Pogliano, K., Linington, R.G., Gutiérrez, M., Lopes, N.P., Gerwick, W.H., Moore, B.S., Dorrestein, P.C. and Bandeira, N. (2016). "Sharing and Community Curation of Mass Spectrometry Data with Global Natural Products Social Molecular Networking". Nat Biotechnol. 34(8):828-837 | PUBMED: #27504778 |
| 7 | 2015 | Aguilar, C., Flores, N., Riveros-McKay, F., Sahonero-Canavesi, D.X., Carmona, S.B., Geiger, O., Escalante, A. and Bolivar, F. (2015). "Deletion of the 2-Acyl-glycerophosphoethanolamine Cycle Improve Glucose Metabolism in Escherichia coli Strains Employed for Overproduction of Aromatic Compounds". Microb Cell Fact. 14(1):194 | PUBMED: #26627477 |
| 8 | 2015 | Escobedo-Hinojosa, W.I., Vences-Guzmán, M.Á., Schubotz, F., Sandoval-Calderón, M., Summons, R.E., López-Lara, I.M., Geiger, O. and Sohlenkamp, C. (2015). "Olsg (sinac_1600) is an Ornithine Lipid N-methyltransferase from the Planctomycete Singulisphaera acidiphila". J Biol Chem. 290(24):15102-15111 | PUBMED: #25925947 |
| 9 | 2015 | Sahonero-Canavesi, D.X., Sohlenkamp, C., Sandoval-Calderón, M., Lamsa, A., Pogliano, K., López-Lara, I.M. and Geiger, O. (2015). "Fatty Acid-releasing Activities In Sinorhizobium meliloti Include Unusual Diacylglycerol Lipase". Environ Microbiol. 17(9):3391-3406 | PUBMED: #25711932 |
| 10 | 2015 | Sandoval-Calderón, M., Nguyen, D.D., Kapono, C.A., Herron, P., Dorrestein, P and Sohlenkamp, C. (2015). "Plasticity Of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions And during Development". Frontiers in Microbiology. 6:1465 | PUBMED: #26733994 |
| 11 | 2015 | Vences-Guzmán, M.Á., Guan, Z.Q., Escobedo-Hinojosa, W.I., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2015). "Discovery Of A Bifunctional Acyltransferase Responsible for Ornithine Lipid Synthesis in Serratia Proteamaculans". Environ Microbiol. 17(5):1487-1496 | PUBMED: #25040623 |
| 12 | 2014 | Semeniuk, A., Sohlenkamp, C., Duda, K. and Holzl, G. (2014). "A Bifunctional Glycosyltransferase from Agrobacterium tumefaciens Synthesizes Monoglucosyl and Glucuronosyl Diacylglycerol under Phosphate Deprivation". J Biol Chem. 289(14):10104-10114 | PUBMED: #24558041 |
| 13 | 2013 | Abdel-Haliem, M.E.F., Sakr, A.A., Ali, M.F., Ghaly, M.F. and Sohlenkamp, C. (2013). "Characterization of Streptomyces Isolates Causing Colour Changes of Mural Paintings in Ancient Egyptian Tombs". Microbiological Research. 168(7):428-437 | PUBMED: #23507492 |
| 14 | 2013 | Geiger, O., López-Lara, I.M. and Sohlenkamp, C. (2013). "Phosphatidylcholine Biosynthesis and Function in Bacteria". Bba-mol Cell Biol L. 1831(3):503-513 | PUBMED: #22922101 |
| 15 | 2013 | Hernández-Tamayo, R., Sohlenkamp, C., Puente, J.L., Brom, S. and Romero, D. (2013). "Characterization of Inta, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42". J Bacteriol. 195(20):4668-4677 | PUBMED: #23935046 |
| 16 | 2013 | Sohlenkamp, C., Raetz, C.R.H. and Ingram, B.O. (2013). "The Calcium-stimulated lipid A 3-o Deacylase from Rhizobium etli is not Essential for Plant Nodulation". Bba-mol Cell Biol L. 1831(7):1250-1259 | PUBMED: #24046865 |
| 17 | 2013 | Vences-Guzmán, M.Á., Guan, Z.Q., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2013). "Agrobacteria Lacking Ornithine Lipids Induce More Rapid Tumour Formation". Environ Microbiol. 15(3):895-906 | PUBMED: #22958119 |
| 18 | 2012 | Estrada-de los Santos, P., Martínez-Aguilar, L., López-Lara, I.M. and Caballero-Mellado, J. (2012). "Cupriavidus Alkaliphilus sp nov., A New Species Associated With Agricultural Plants that Grow in Alkaline Soils". Syst Appl Microbiol. 35(5):310-314 | PUBMED: #22738764 |
| 19 | 2012 | Higareda, M.F., Geiger, O., Mendoza, L. and Mendez-Sánchez, R.A. (2012). "Evidence of Codon Usage in the Nearest Neighbor Spacing Distribution of Bases in Bacterial Genomes". Physica a. 391(4):1255-1269 | |
| 20 | 2012 | Solis-Oviedo, R.L., Martínez-Morales, F., Geiger, O. and Sohlenkamp, C. (2012). "Functional And Topological Analysis of Phosphatidylcholine Synthase from Sinorhizobium meliloti". Bba-mol Cell Biol L. 1821(4):573-581 | PUBMED: #22333179 |
| 21 | 2012 | Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2012). "Ornithine Lipids and their Structural Modifications: from a to e and beyond". FEMS Microbiol Lett. 335(1):1-10 | PUBMED: #22724388 |
| 22 | 2011 | González-Silva, N., López-Lara, I.M., Reyes-Lamothe, R., Taylor, A.M., Sumpton, D., Thomas-Oates, J. and Geiger, O. (2011). "The Dioxygenase-encoding Olsd Gene from Burkholderia cenocepacia Causes the Hydroxylation of the Amide-linked Fatty Acyl Moiety of Ornithine-containing Membrane Lipids". Biochemistry-us. 50(29):6396-6408 | PUBMED: #21707055 |
| 23 | 2011 | Landeta, C., Dávalos, A., Cevallos, M.A., Geiger, O., Brom, S. and Romero, D. (2011). "Plasmids With a Chromosome-like Role in Rhizobia". J Bacteriol. 193(6):1317-1326 | PUBMED: #21217003 |
| 24 | 2011 | Pech-Canul, A., Nogales, J., Miranda-Molina, A., Álvarez, L., Geiger, O., Soto, M.J. and López-Lara, I.M. (2011). "FadD is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids". J Bacteriol. 193(22):6295-6304 | PUBMED: #21926226 |
| 25 | 2011 | Vences-Guzmán, M.Á., Guan, Z.Q., Ormeño-Orrillo, E., González-Silva, N., López-Lara, I.M., Martínez-Romero, E., Geiger, O. and Sohlenkamp, C. (2011). "Hydroxylated Ornithine Lipids Increase Stress Tolerance In Rhizobium tropici CIAT899". Mol Microbiol. 79(6):1496-1514 | PUBMED: #21205018 |
| 26 | 2010 | Dávila-Martínez, Y., Ramos-Vega, A.L., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2010). "Smc01553 Is The Sixth Acyl Carrier Protein in Sinorhizobium meliloti 1021". Microbiology-sgm. 156:230-239 | PUBMED: #19797355 |
| 27 | 2010 | Geiger, O., González-Silva, N., López-Lara, I.M. and Sohlenkamp, C. (2010). "Amino Acid-containing Membrane Lipids in Bacteria". Prog Lipid Res. | PUBMED: #19703488 |
| 28 | 2010 | Ingram, B.O., Sohlenkamp, C., Geiger, O. and Raetz, C.R.H. (2010). "Altered lipid A Structures and Polymyxin Hypersensitivity of Rhizobium etli Mutants Lacking the lpxE and LpxF Phosphatases". Bba-mol Cell Biol L. 1801(5):593-604 | PUBMED: #20153447 |
| 29 | 2010 | Medeot, D.B., Sohlenkamp, C., Dardanelli, M.S., Geiger, O., de Lema, M.G. and López-Lara, I.M. (2010). "Phosphatidylcholine Levels Of Peanut-nodulating Bradyrhizobium sp SEMIA 6144 Affect Cell Size and Motility". FEMS Microbiol Lett. 303(2):123-131 | PUBMED: #20030724 |
| 30 | 2010 | Zavaleta-Pastor, M., Sohlenkamp, C., Gao, J.L., Guan, Z.Q., Zaheer, R., Finan, T.M., Raetz, C.R.H., López-Lara, I.M. and Geiger, O. (2010). "Sinorhizobium meliloti Phospholipase C Required For Lipid Remodeling during Phosphorus Limitation". P Nat Acad Sci USA. 107(1):302-307 | PUBMED: #20018679 |
| 31 | 2009 | Ramos-Vega, A.L., Dávila-Martínez, Y., Sohlenkamp, C., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2009). "SMb20651 Is Another Acyl Carrier Protein from Sinorhizobium meliloti". Microbiology. 155:257-267 | PUBMED: #19118366 |
| 32 | 2009 | Sandoval-Calderón, M., Geiger, O., Guan, Z. Q., Barona-Gómez, F. and Sohlenkamp, C. (2009). "A Eukaryote-like Cardiolipin Synthase is Present in Streptomyces coelicolor and in Most Actinobacteria". J Biol Chem. 284(26):17383-17390 | PUBMED: #19439403 |
| 33 | 2008 | Conover, G.M., Martinez-Morales, F., Heidtman, M.I., Luo, Z.Q., Tang, M., Chen, C., Geiger, O. and Isberg, R.R. (2008). "Phosphatidylcholine Synthesis Is Required for Optimal Function of Legionella pneumophila Virulence Determinants". Cell Microbiol. 10(2):514-528 | PUBMED: #17979985 |
| 34 | 2008 | Hacker, S., Sohlenkamp, C., Aktas, M., Geiger, O. and Narberhaus, F. (2008). "Multiple Phospholipid N-Methyltransferases With Distinct Substrate Specificities Are Encoded in Bradyrhizobium japonicum". J Bacteriol. 190(2):571-580 | PUBMED: #17993534 |
| 35 | 2008 | Sepúlveda, E., Perez-Mendoza, D., Ramírez-Romero, M.Á., Soto, M.J., López-Lara, I.M., Geiger, O., Sanjuan, J., Brom, S. and Romero, D. (2008). "Transcriptional Interference And Repression Modulate the Conjugative Ability of the Symbiotic Plasmid of Rhizobium etli". J Bacteriol. 190(12):4189-4197 | PUBMED: #18424522 |
| 36 | 2008 | Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2008). "Sinorhizobium meliloti Mutants Deficient In Phosphatidylserine Decarboxylase Accumulate Phosphatidylserine And are Strongly Affected during Symbiosis with Alfalfa". J Bacteriol. 190(20):6846-6856 | PUBMED: #18708506 |
| 37 | 2007 | Medeot, D.B., Sohlenkamp, C., Geiger, O., García, M.B. and López-Lara, I.M. (2007). "Phosphatidycholine Fuction in Rhizobial Strains Nodulating Peanut: Genes and Enzymatic Activites". Biocell. 31:134 | |
| 38 | 2007 | Sohlenkamp, C., Galindo-Lagunas, K.A., Guan, Z.Q., Vinuesa, P., Robinson, S., Thomas-Oates, J., Raetz, C.R.H. and Geiger, O. (2007). "The Lipid Lysyl-phosphatidylglycerol Is Present In Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B under Acidic Growth Conditions". Mol Plant Microbe Interactions. 20(11):1421-1430 | PUBMED: #17977153 |
| 39 | 2006 | López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2006). "The Post-genomic Era--what Comes next?". Revista Latinoamericana de Microbiología. 48(1):44-45 | PUBMED: #17357573 |
| 40 | 2006 | Reeve, W.G., Brau, L., Castelli, J., Garau, G., Sohlenkamp, C., Geiger, O., Dilworth, M.J., Glenn, A.R., Howieson, J.G. and Tiwaril, R.P. (2006). "The Sinorhizobium Medicae WSM419 Ipia Gene is Transcriptionally Activated by FsrR and Required to Enhance Survival in Lethal Acid Conditions". Microbiology-sgm. 152:3049-3059 | PUBMED: #17005985 |
| 41 | 2005 | López-Lara, I.M., Gao, J.L., Soto, M.J., Solares-Pérez, A., Weissenmayer, B., Sohlenkamp, C., Verroios, G.P., Thomas-Oates, J. and Geiger, O. (2005). "Phosphorus-free Membrane Lipids of Sinorhizobium meliloti are not Required for the Symbiosis with Alfalfa but Contribute to Increased Cell Yields under Phosphorus-limiting Conditions of Growth". Mol Plant Microbe Interactions. 18(9):973-982 | PUBMED: #16167767 |
| 42 | 2005 | Rojas-Jimenez, K., Sohlenkamp, C., Geiger, O., Martínez-Romero, E., Werner, D. and Vinuesa, P. (2005). "A Cic Chloride Channel Homolog and Ornithine-containing Membrane Lipids of Rhizobium tropici CIAT899 are Involved in Symbiotic Efficiency and Acid Tolerance". Mol Plant Microbe Interactions. 18(11):1175-1185 | PUBMED: #16353552 |
| 43 | 2004 | Gao, J.L., Weissenmayer, B., Taylor, A.M., Thomas-Oates, J., López-Lara, I.M. and Geiger, O. (2004). "Identification of a Gene Required for the Formation of Lyso-ornithine Lipid, an Intermediate in the Biosynthesis of Ornithine-containing Lipids". Mol Microbiol. 53(6):1757-1770 | PUBMED: #15341653 |
| 44 | 2004 | Sohlenkamp, C., de Rudder, K.E.E. and Geiger, O. (2004). "Phosphatidylethanolamine Is not Essential for Growth of Sinorhizobium meliloti on Complex Culture Media". J Bacteriol. 186(6):1667-1677 | PUBMED: #14996797 |
| 45 | 2003 | Djordjevic, M.A., Chen, H.C., Natera, S., Van Noorden, G., Menzel, C., Taylor, S., Renard, C., Geiger, O. and Weiller, G.F. (2003). "A Global Analysis of Protein Expression Profiles in Sinorhizobium meliloti: Discovery of New Genes for Nodule Occupancy and Stress Adaptation". Mol Plant Microbe Interactions. 16(6):508-524 | PUBMED: #12795377 |
| 46 | 2003 | López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2003). "Membrane Lipids in Plant-associated Bacteria: their Biosyntheses and Possible Functions". Mol Plant Microbe Interactions. 16(7):567-579 | PUBMED: #12848422 |
| 47 | 2003 | Martínez-Morales, F., Schobert, M., López-Lara, I.M. and Geiger, O. (2003). "Pathways for Phosphatidylcholine Biosynthesis in Bacteria". Microbiology-sgm. 149:3461-3471 | PUBMED: #14663079 |
| 48 | 2003 | Sohlenkamp, C., López-Lara, I.M. and Geiger, O. (2003). "Biosynthesis of Phosphatidylcholine in Bacteria". Prog Lipid Res. 42(2):115-162 | PUBMED: #12547654 |
| 49 | 2002 | Geiger, O. and López-Lara, I.M. (2002). "Rhizobial Acyl Carrier Proteins and their Roles in the Formation of Bacterial Cell-surface Components that are Required for the Development of Nitrogen-fixing Root Nodules on Legume Hosts". FEMS Microbiol Lett. 208(2):153-162 | PUBMED: #11959430 |
| 50 | 2002 | Sohlenkamp, C., Wood, C.C., Roeb, G.W. and Udvardi, M.K. (2002). "Characterization of Arabidopsis AtAMT2, A High-affinity Ammonium Transporter of the Plasma Membrane". Plant Physiol. 130(4):1788-1796 | PUBMED: #12481062 |
| 51 | 2002 | Weissenmayer, B., Gao, J.L., López-Lara, I.M. and Geiger, O. (2002). "Identification of a Gene Required for the Biosynthesis of Ornithine-derived Lipids". Mol Microbiol. 45(3):721-733 | PUBMED: #12139618 |
| 52 | 2001 | López-Lara, I.M. and Geiger, O. (2001). "Novel Pathway for Phosphatidylcholine Biosynthesis in Bacteria Associated with Eukaryotes". J Biotechnol. 91(2-3):211-221 | PUBMED: #11566392 |
| 53 | 2001 | López-Lara, I.M. and Geiger, O. (2001). "The Nodulation Protein NodG Shows the Enzymatic Activity of an 3-Oxoacyl-Acyl Carrier Protein Reductase". Mol Plant Microbe Interactions. 14(3):349-357 | PUBMED: #11277432 |
| 54 | 2001 | Minder, A.C., de Rudder, K.E.E., Narberhaus, F., Fischer, H.M., Hennecke, H. and Geiger, O. (2001). "Phosphatidylcholine Levels In Bradyrhizobium japonicum Membranes Are Critical for an Efficient Symbiosis with the Soybean Host Plant". Mol Microbiol. 39(5):1186-1198 | PUBMED: #11251836 |
| 55 | 2000 | de Rudder, K.E.E., López-Lara, I.M. and Geiger, O. (2000). "Inactivation Of the Gene for Phospholipid N-methyltransferase in Sinorhizobium meliloti: Phosphatidylcholine is Required for Normal Growth". Mol Microbiol. 37(4):763-772 | PUBMED: #10972799 |
| 56 | 2000 | Sohlenkamp, C., de Rudder, K.E.E., Rohrs, V., López-Lara, I.M. and Geiger, O. (2000). "Cloning and Characterization of the Gene for Phosphatidylcholine Synthase". J Biol Chem. 275(25):18919-18925 | PUBMED: #10858449 |
| 57 | 2000 | Weissenmayer, B., Geiger, O. and Benning, C. (2000). "Disruption Of a Gene Essential for Sulfoquinovosyldiacylglycerol Biosynthesis in Sinorhizobium meliloti Has no Detectable Effect on Root Nodule Symbiosis". Mol Plant Microbe Interactions. 13(6):666-672 | PUBMED: #10830266 |
Formation of new membrane lipids in rhizobia under stress conditions
Principal investigator: Christian Sohlenkamp
Participants: Otto Geiger, Isabel María López-Lara, Miguel Angel Vences-Guzmán
Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation
Post-genomic studies in Sinorhizobium meliloti directed to discover new biosynthesis pathways related to beta-ketide formation
Participants: Otto Geiger, Christian Sohlenkamp, Ana Laura Ramos-Vega, Yadira Dávila-Martínez, Sandra Leticia Beltrán-Escobar
Biosynthesis of ornithine-containing lipids, a widespread class of bioactive lipids in eubacterial membranes
Principal investigator: Otto Geiger
Biosynthesis of choline-derived bacterial cell surface components...
Biosynthesis of choline-derived bacterial cell surface components: an essential requirement for the success of many pathogenic bacteria
Principal investigator: Otto Geiger
Formation, function, and replacement of membrane lipids in rhizobia
Principal investigator: Otto Geiger
Interactions between Pro- and Eukaryotes Research Group
Group Responsible: Otto Geiger, Ph.D.
The research group
By the time this Research Group joined the Nitrogen Fixation Research Center in 1999, the laboratory of Otto Geiger had discovered the phosphatidylcholine synthase pathway, which constitutes a major route for phosphatidylcholine (lecithine) formation in bacteria. This finding started a research line aimed to understand how molecules of the bacterial membrane or cell surface modify defense responses of eukaryotic hosts thus allowing long-lasting or permanent infections which can lead to symbiotic or pathogenic interactions with the host.
Publications
| # | Year | Title | Links |
| 1 | 2017 | López-Lara, I.M. and Geiger, O. (2017). "Bacterial Lipid Diversity". Bba-mol Cell Biol L. 1862(11):1287-1299 | PUBMED: #27760387 |
| 2 | 2017 | Sandoval-Calderón, M., Guan, Z.Q. and Sohlenkamp, C. (2017). "Knowns and Unknowns of Membrane Lipid Synthesis in Streptomycetes". Biochimie. 141:21-29 | PUBMED: #28522365 |
| 3 | 2017 | Vences-Guzmán, M.Á., Goetting-Minesky, M.P., Guan, Z.Q., Castillo-Ramírez, S., Cordoba-Castro, L.A., López-Lara, I.M., Geiger, O., Sohlenkamp, C. and Fenno, J.C. (2017). "1,2-Diacylglycerol Choline Phosphotransferase Catalyzes the Final Step In the Unique Treponema denticola Phosphatidylcholine Biosynthesis Pathway". Mol Microbiol. 103(5):896-912 | PUBMED: #28009086 |
| 4 | 2016 | Sahonero-Canavesi, D.X., Zavaleta-Pastor, M., Martínez-Aguilar, L., López-Lara, I.M. and Geiger, O. (2016). "Defining Substrate Specificities for Lipase and Phospholipase Candidates". Jove-journal of Visualized Experiments. (117)-e54613 | PUBMED: #27911408 |
| 5 | 2016 | Sohlenkamp, C. and Geiger, O. (2016). "Bacterial Membrane Lipids: Diversity in Structures and Pathways". FEMS Microbiol Rev. 40(1):133-159 | PUBMED: #25862689 |
| 6 | 2016 | Wang, M.X., Carver, J.J., Phelan, V.V., Sánchez, L.M., Garg, N., Peng, Y., Nguyen, D.D., Watrous, J., Kapono, C.A., Luzzatto-Knaan, T., Porto, C., Bouslimani, A., Melnik, A.V., Meehan, M.J., Liu, W.T., Criisemann, M., Boudreau, P.D., Esquenazi, E., Sandoval-Calderón, M., Kersten, R.D., Pace, L.A., Quinn, R.A., Duncan, K.R., Hsu, C.C., Floros, D.J., Gavilan, R.G., Kleigrewe, K., Northen, T., Dutton, R.J., Parrot, D., Carlson, E.E., Aigle, B., Michelsen, C.F., Jelsbak, L., Sohlenkamp, C., Pevzner, P., Edlund, A., McLean, J., Piel, J., Murphy, B.T., Gerwick, L., Liaw, C.C., Yang, Y.L., Humpf, H.U., Maansson, M., Keyzers, R.A., Sims, A.C., Johnson, A.R., Sidebottom, A.M., Sedio, B.E., Klitgaard, A., Larson, C.B., Boya, C.A., Torres-Mendoza, D., González, D.J., Silva, D.B., Marques, L.M., Demarque, D.P., Pociute, E., ONeill, E.C., Helfrich, E.J.N., Granatosky, E.A., Glukhov, E., Ryffel, F., Houson, H., Mohimani, H., Kharbush, J.J., Zeng, Y., Vorholt, J.A., Kurita, K.L., Charusanti, P., McPhail, K.L., Nielsen, K.F., Vuong, L., Elfeki, M., Traxler, M.F., Engene, N., Koyama, N., Vining, O.B., Baric, R., Silva, R.R., Mascuch, S.J., Tomasi, S., Jenkins, S., Macherla, V., Hoffman, T., Agarwal, V., Williams, P.G., Dai, J.Q., Neupane, R., Gurr, J., Rodríguez, A.M.C., Lamsa, A., Zhang, C., Dorrestein, K., Duggan, B.M., Almaliti, J., Allard, P.M., Phapale, P., Nothias, L.F., Alexandrovr, T., Litaudon, M., Wolfender, J.L., Kyle, J.E., Metz, T.O., Peryea, T., Nguyen, D.T., VanLeer, D., Shinn, P., Jadhav, A., Muller, R., Waters, K.M., Shi, W.Y., Liu, X.T., Zhang, L.X., Knight, R., Jensen, P.R., Palsson, B.O., Pogliano, K., Linington, R.G., Gutiérrez, M., Lopes, N.P., Gerwick, W.H., Moore, B.S., Dorrestein, P.C. and Bandeira, N. (2016). "Sharing and Community Curation of Mass Spectrometry Data with Global Natural Products Social Molecular Networking". Nat Biotechnol. 34(8):828-837 | PUBMED: #27504778 |
| 7 | 2015 | Aguilar, C., Flores, N., Riveros-McKay, F., Sahonero-Canavesi, D.X., Carmona, S.B., Geiger, O., Escalante, A. and Bolivar, F. (2015). "Deletion of the 2-Acyl-glycerophosphoethanolamine Cycle Improve Glucose Metabolism in Escherichia coli Strains Employed for Overproduction of Aromatic Compounds". Microb Cell Fact. 14(1):194 | PUBMED: #26627477 |
| 8 | 2015 | Escobedo-Hinojosa, W.I., Vences-Guzmán, M.Á., Schubotz, F., Sandoval-Calderón, M., Summons, R.E., López-Lara, I.M., Geiger, O. and Sohlenkamp, C. (2015). "Olsg (sinac_1600) is an Ornithine Lipid N-methyltransferase from the Planctomycete Singulisphaera acidiphila". J Biol Chem. 290(24):15102-15111 | PUBMED: #25925947 |
| 9 | 2015 | Sahonero-Canavesi, D.X., Sohlenkamp, C., Sandoval-Calderón, M., Lamsa, A., Pogliano, K., López-Lara, I.M. and Geiger, O. (2015). "Fatty Acid-releasing Activities In Sinorhizobium meliloti Include Unusual Diacylglycerol Lipase". Environ Microbiol. 17(9):3391-3406 | PUBMED: #25711932 |
| 10 | 2015 | Sandoval-Calderón, M., Nguyen, D.D., Kapono, C.A., Herron, P., Dorrestein, P and Sohlenkamp, C. (2015). "Plasticity Of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions And during Development". Frontiers in Microbiology. 6:1465 | PUBMED: #26733994 |
| 11 | 2015 | Vences-Guzmán, M.Á., Guan, Z.Q., Escobedo-Hinojosa, W.I., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2015). "Discovery Of A Bifunctional Acyltransferase Responsible for Ornithine Lipid Synthesis in Serratia Proteamaculans". Environ Microbiol. 17(5):1487-1496 | PUBMED: #25040623 |
| 12 | 2014 | Semeniuk, A., Sohlenkamp, C., Duda, K. and Holzl, G. (2014). "A Bifunctional Glycosyltransferase from Agrobacterium tumefaciens Synthesizes Monoglucosyl and Glucuronosyl Diacylglycerol under Phosphate Deprivation". J Biol Chem. 289(14):10104-10114 | PUBMED: #24558041 |
| 13 | 2013 | Abdel-Haliem, M.E.F., Sakr, A.A., Ali, M.F., Ghaly, M.F. and Sohlenkamp, C. (2013). "Characterization of Streptomyces Isolates Causing Colour Changes of Mural Paintings in Ancient Egyptian Tombs". Microbiological Research. 168(7):428-437 | PUBMED: #23507492 |
| 14 | 2013 | Geiger, O., López-Lara, I.M. and Sohlenkamp, C. (2013). "Phosphatidylcholine Biosynthesis and Function in Bacteria". Bba-mol Cell Biol L. 1831(3):503-513 | PUBMED: #22922101 |
| 15 | 2013 | Hernández-Tamayo, R., Sohlenkamp, C., Puente, J.L., Brom, S. and Romero, D. (2013). "Characterization of Inta, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42". J Bacteriol. 195(20):4668-4677 | PUBMED: #23935046 |
| 16 | 2013 | Sohlenkamp, C., Raetz, C.R.H. and Ingram, B.O. (2013). "The Calcium-stimulated lipid A 3-o Deacylase from Rhizobium etli is not Essential for Plant Nodulation". Bba-mol Cell Biol L. 1831(7):1250-1259 | PUBMED: #24046865 |
| 17 | 2013 | Vences-Guzmán, M.Á., Guan, Z.Q., Bermúdez-Barrientos, J.R., Geiger, O. and Sohlenkamp, C. (2013). "Agrobacteria Lacking Ornithine Lipids Induce More Rapid Tumour Formation". Environ Microbiol. 15(3):895-906 | PUBMED: #22958119 |
| 18 | 2012 | Estrada-de los Santos, P., Martínez-Aguilar, L., López-Lara, I.M. and Caballero-Mellado, J. (2012). "Cupriavidus Alkaliphilus sp nov., A New Species Associated With Agricultural Plants that Grow in Alkaline Soils". Syst Appl Microbiol. 35(5):310-314 | PUBMED: #22738764 |
| 19 | 2012 | Higareda, M.F., Geiger, O., Mendoza, L. and Mendez-Sánchez, R.A. (2012). "Evidence of Codon Usage in the Nearest Neighbor Spacing Distribution of Bases in Bacterial Genomes". Physica a. 391(4):1255-1269 | |
| 20 | 2012 | Solis-Oviedo, R.L., Martínez-Morales, F., Geiger, O. and Sohlenkamp, C. (2012). "Functional And Topological Analysis of Phosphatidylcholine Synthase from Sinorhizobium meliloti". Bba-mol Cell Biol L. 1821(4):573-581 | PUBMED: #22333179 |
| 21 | 2012 | Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2012). "Ornithine Lipids and their Structural Modifications: from a to e and beyond". FEMS Microbiol Lett. 335(1):1-10 | PUBMED: #22724388 |
| 22 | 2011 | González-Silva, N., López-Lara, I.M., Reyes-Lamothe, R., Taylor, A.M., Sumpton, D., Thomas-Oates, J. and Geiger, O. (2011). "The Dioxygenase-encoding Olsd Gene from Burkholderia cenocepacia Causes the Hydroxylation of the Amide-linked Fatty Acyl Moiety of Ornithine-containing Membrane Lipids". Biochemistry-us. 50(29):6396-6408 | PUBMED: #21707055 |
| 23 | 2011 | Landeta, C., Dávalos, A., Cevallos, M.A., Geiger, O., Brom, S. and Romero, D. (2011). "Plasmids With a Chromosome-like Role in Rhizobia". J Bacteriol. 193(6):1317-1326 | PUBMED: #21217003 |
| 24 | 2011 | Pech-Canul, A., Nogales, J., Miranda-Molina, A., Álvarez, L., Geiger, O., Soto, M.J. and López-Lara, I.M. (2011). "FadD is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids". J Bacteriol. 193(22):6295-6304 | PUBMED: #21926226 |
| 25 | 2011 | Vences-Guzmán, M.Á., Guan, Z.Q., Ormeño-Orrillo, E., González-Silva, N., López-Lara, I.M., Martínez-Romero, E., Geiger, O. and Sohlenkamp, C. (2011). "Hydroxylated Ornithine Lipids Increase Stress Tolerance In Rhizobium tropici CIAT899". Mol Microbiol. 79(6):1496-1514 | PUBMED: #21205018 |
| 26 | 2010 | Dávila-Martínez, Y., Ramos-Vega, A.L., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2010). "Smc01553 Is The Sixth Acyl Carrier Protein in Sinorhizobium meliloti 1021". Microbiology-sgm. 156:230-239 | PUBMED: #19797355 |
| 27 | 2010 | Geiger, O., González-Silva, N., López-Lara, I.M. and Sohlenkamp, C. (2010). "Amino Acid-containing Membrane Lipids in Bacteria". Prog Lipid Res. | PUBMED: #19703488 |
| 28 | 2010 | Ingram, B.O., Sohlenkamp, C., Geiger, O. and Raetz, C.R.H. (2010). "Altered lipid A Structures and Polymyxin Hypersensitivity of Rhizobium etli Mutants Lacking the lpxE and LpxF Phosphatases". Bba-mol Cell Biol L. 1801(5):593-604 | PUBMED: #20153447 |
| 29 | 2010 | Medeot, D.B., Sohlenkamp, C., Dardanelli, M.S., Geiger, O., de Lema, M.G. and López-Lara, I.M. (2010). "Phosphatidylcholine Levels Of Peanut-nodulating Bradyrhizobium sp SEMIA 6144 Affect Cell Size and Motility". FEMS Microbiol Lett. 303(2):123-131 | PUBMED: #20030724 |
| 30 | 2010 | Zavaleta-Pastor, M., Sohlenkamp, C., Gao, J.L., Guan, Z.Q., Zaheer, R., Finan, T.M., Raetz, C.R.H., López-Lara, I.M. and Geiger, O. (2010). "Sinorhizobium meliloti Phospholipase C Required For Lipid Remodeling during Phosphorus Limitation". P Nat Acad Sci USA. 107(1):302-307 | PUBMED: #20018679 |
| 31 | 2009 | Ramos-Vega, A.L., Dávila-Martínez, Y., Sohlenkamp, C., Contreras-Martínez, S., Encarnación, S., Geiger, O. and López-Lara, I.M. (2009). "SMb20651 Is Another Acyl Carrier Protein from Sinorhizobium meliloti". Microbiology. 155:257-267 | PUBMED: #19118366 |
| 32 | 2009 | Sandoval-Calderón, M., Geiger, O., Guan, Z. Q., Barona-Gómez, F. and Sohlenkamp, C. (2009). "A Eukaryote-like Cardiolipin Synthase is Present in Streptomyces coelicolor and in Most Actinobacteria". J Biol Chem. 284(26):17383-17390 | PUBMED: #19439403 |
| 33 | 2008 | Conover, G.M., Martinez-Morales, F., Heidtman, M.I., Luo, Z.Q., Tang, M., Chen, C., Geiger, O. and Isberg, R.R. (2008). "Phosphatidylcholine Synthesis Is Required for Optimal Function of Legionella pneumophila Virulence Determinants". Cell Microbiol. 10(2):514-528 | PUBMED: #17979985 |
| 34 | 2008 | Hacker, S., Sohlenkamp, C., Aktas, M., Geiger, O. and Narberhaus, F. (2008). "Multiple Phospholipid N-Methyltransferases With Distinct Substrate Specificities Are Encoded in Bradyrhizobium japonicum". J Bacteriol. 190(2):571-580 | PUBMED: #17993534 |
| 35 | 2008 | Sepúlveda, E., Perez-Mendoza, D., Ramírez-Romero, M.Á., Soto, M.J., López-Lara, I.M., Geiger, O., Sanjuan, J., Brom, S. and Romero, D. (2008). "Transcriptional Interference And Repression Modulate the Conjugative Ability of the Symbiotic Plasmid of Rhizobium etli". J Bacteriol. 190(12):4189-4197 | PUBMED: #18424522 |
| 36 | 2008 | Vences-Guzmán, M.Á., Geiger, O. and Sohlenkamp, C. (2008). "Sinorhizobium meliloti Mutants Deficient In Phosphatidylserine Decarboxylase Accumulate Phosphatidylserine And are Strongly Affected during Symbiosis with Alfalfa". J Bacteriol. 190(20):6846-6856 | PUBMED: #18708506 |
| 37 | 2007 | Medeot, D.B., Sohlenkamp, C., Geiger, O., García, M.B. and López-Lara, I.M. (2007). "Phosphatidycholine Fuction in Rhizobial Strains Nodulating Peanut: Genes and Enzymatic Activites". Biocell. 31:134 | |
| 38 | 2007 | Sohlenkamp, C., Galindo-Lagunas, K.A., Guan, Z.Q., Vinuesa, P., Robinson, S., Thomas-Oates, J., Raetz, C.R.H. and Geiger, O. (2007). "The Lipid Lysyl-phosphatidylglycerol Is Present In Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B under Acidic Growth Conditions". Mol Plant Microbe Interactions. 20(11):1421-1430 | PUBMED: #17977153 |
| 39 | 2006 | López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2006). "The Post-genomic Era--what Comes next?". Revista Latinoamericana de Microbiología. 48(1):44-45 | PUBMED: #17357573 |
| 40 | 2006 | Reeve, W.G., Brau, L., Castelli, J., Garau, G., Sohlenkamp, C., Geiger, O., Dilworth, M.J., Glenn, A.R., Howieson, J.G. and Tiwaril, R.P. (2006). "The Sinorhizobium Medicae WSM419 Ipia Gene is Transcriptionally Activated by FsrR and Required to Enhance Survival in Lethal Acid Conditions". Microbiology-sgm. 152:3049-3059 | PUBMED: #17005985 |
| 41 | 2005 | López-Lara, I.M., Gao, J.L., Soto, M.J., Solares-Pérez, A., Weissenmayer, B., Sohlenkamp, C., Verroios, G.P., Thomas-Oates, J. and Geiger, O. (2005). "Phosphorus-free Membrane Lipids of Sinorhizobium meliloti are not Required for the Symbiosis with Alfalfa but Contribute to Increased Cell Yields under Phosphorus-limiting Conditions of Growth". Mol Plant Microbe Interactions. 18(9):973-982 | PUBMED: #16167767 |
| 42 | 2005 | Rojas-Jimenez, K., Sohlenkamp, C., Geiger, O., Martínez-Romero, E., Werner, D. and Vinuesa, P. (2005). "A Cic Chloride Channel Homolog and Ornithine-containing Membrane Lipids of Rhizobium tropici CIAT899 are Involved in Symbiotic Efficiency and Acid Tolerance". Mol Plant Microbe Interactions. 18(11):1175-1185 | PUBMED: #16353552 |
| 43 | 2004 | Gao, J.L., Weissenmayer, B., Taylor, A.M., Thomas-Oates, J., López-Lara, I.M. and Geiger, O. (2004). "Identification of a Gene Required for the Formation of Lyso-ornithine Lipid, an Intermediate in the Biosynthesis of Ornithine-containing Lipids". Mol Microbiol. 53(6):1757-1770 | PUBMED: #15341653 |
| 44 | 2004 | Sohlenkamp, C., de Rudder, K.E.E. and Geiger, O. (2004). "Phosphatidylethanolamine Is not Essential for Growth of Sinorhizobium meliloti on Complex Culture Media". J Bacteriol. 186(6):1667-1677 | PUBMED: #14996797 |
| 45 | 2003 | Djordjevic, M.A., Chen, H.C., Natera, S., Van Noorden, G., Menzel, C., Taylor, S., Renard, C., Geiger, O. and Weiller, G.F. (2003). "A Global Analysis of Protein Expression Profiles in Sinorhizobium meliloti: Discovery of New Genes for Nodule Occupancy and Stress Adaptation". Mol Plant Microbe Interactions. 16(6):508-524 | PUBMED: #12795377 |
| 46 | 2003 | López-Lara, I.M., Sohlenkamp, C. and Geiger, O. (2003). "Membrane Lipids in Plant-associated Bacteria: their Biosyntheses and Possible Functions". Mol Plant Microbe Interactions. 16(7):567-579 | PUBMED: #12848422 |
| 47 | 2003 | Martínez-Morales, F., Schobert, M., López-Lara, I.M. and Geiger, O. (2003). "Pathways for Phosphatidylcholine Biosynthesis in Bacteria". Microbiology-sgm. 149:3461-3471 | PUBMED: #14663079 |
| 48 | 2003 | Sohlenkamp, C., López-Lara, I.M. and Geiger, O. (2003). "Biosynthesis of Phosphatidylcholine in Bacteria". Prog Lipid Res. 42(2):115-162 | PUBMED: #12547654 |
| 49 | 2002 | Geiger, O. and López-Lara, I.M. (2002). "Rhizobial Acyl Carrier Proteins and their Roles in the Formation of Bacterial Cell-surface Components that are Required for the Development of Nitrogen-fixing Root Nodules on Legume Hosts". FEMS Microbiol Lett. 208(2):153-162 | PUBMED: #11959430 |
| 50 | 2002 | Sohlenkamp, C., Wood, C.C., Roeb, G.W. and Udvardi, M.K. (2002). "Characterization of Arabidopsis AtAMT2, A High-affinity Ammonium Transporter of the Plasma Membrane". Plant Physiol. 130(4):1788-1796 | PUBMED: #12481062 |
| 51 | 2002 | Weissenmayer, B., Gao, J.L., López-Lara, I.M. and Geiger, O. (2002). "Identification of a Gene Required for the Biosynthesis of Ornithine-derived Lipids". Mol Microbiol. 45(3):721-733 | PUBMED: #12139618 |
| 52 | 2001 | López-Lara, I.M. and Geiger, O. (2001). "Novel Pathway for Phosphatidylcholine Biosynthesis in Bacteria Associated with Eukaryotes". J Biotechnol. 91(2-3):211-221 | PUBMED: #11566392 |
| 53 | 2001 | López-Lara, I.M. and Geiger, O. (2001). "The Nodulation Protein NodG Shows the Enzymatic Activity of an 3-Oxoacyl-Acyl Carrier Protein Reductase". Mol Plant Microbe Interactions. 14(3):349-357 | PUBMED: #11277432 |
| 54 | 2001 | Minder, A.C., de Rudder, K.E.E., Narberhaus, F., Fischer, H.M., Hennecke, H. and Geiger, O. (2001). "Phosphatidylcholine Levels In Bradyrhizobium japonicum Membranes Are Critical for an Efficient Symbiosis with the Soybean Host Plant". Mol Microbiol. 39(5):1186-1198 | PUBMED: #11251836 |
| 55 | 2000 | de Rudder, K.E.E., López-Lara, I.M. and Geiger, O. (2000). "Inactivation Of the Gene for Phospholipid N-methyltransferase in Sinorhizobium meliloti: Phosphatidylcholine is Required for Normal Growth". Mol Microbiol. 37(4):763-772 | PUBMED: #10972799 |
| 56 | 2000 | Sohlenkamp, C., de Rudder, K.E.E., Rohrs, V., López-Lara, I.M. and Geiger, O. (2000). "Cloning and Characterization of the Gene for Phosphatidylcholine Synthase". J Biol Chem. 275(25):18919-18925 | PUBMED: #10858449 |
| 57 | 2000 | Weissenmayer, B., Geiger, O. and Benning, C. (2000). "Disruption Of a Gene Essential for Sulfoquinovosyldiacylglycerol Biosynthesis in Sinorhizobium meliloti Has no Detectable Effect on Root Nodule Symbiosis". Mol Plant Microbe Interactions. 13(6):666-672 | PUBMED: #10830266 |