Susana Brom Klanner

Genome Engineering Researcher

Associate Professor

Academic history

Name: Susana Brom Klanner (Associate Professor).

Academic history
Undergraduate (B Sc.): Basic Biomedical Research, U.N.A.M. 1980.
Isolation and characterization of mutations in the glnA region of the E. coli K12 chromosome.
Masters (M Sc.): Basic Biomedical Research, National Autonomous University of Mexico, 1982. Characterization of mutations in the glnA region of the E. coli K12 chromosome

Ph.D.Basic Biomedical Research, National Autonomous University of Mexico, 1988.
Analisis of symbiotic plasmids in Rhizobium phaseoli.

Initial research position
Assistant professor, Nitrogen Fixation Research Center, National Autonomous University of Mexico, 1983- 1993

Research line
Rhizobium are gram negative bacteria, able to form a symbiotic relationship with the roots of leguminous plants. Specifically, Rhizobium etli interacts with the roots of Phaseolus vulgaris (beans). The genome of the R. etli type strain CFN42 consists of a chromosome and 6 plasmids, varying in size between 180 and 600 Kb. We are interested in:

1- Further analyzing the functions encoded on plasmids. Previously we have found that plasmid encoded information is required for functions such as optimal competitive capacity for nodulation, saprophytic growth, lipopolysaccharide production, and one of the plasmids even seems to contain information which is indispensable for survival.
2- Analyzing the different mechanisms that contribute to symbiotic plasmid transfer, and their regulation.
3- Search for the presence of site-specific recombination systems, similar to the one we found on R. etli CFN42 plasmids; on other Rhizobiacea.

This work will contribute to a better understanding of the role of plasmids in the rhizobial life cycle, through the knowledge of plasmid encoded functions, and of the mechanisms that contribute to the distribution of symbiotic plasmids and the elements and conditions that control these processes.


My work has contributed to the description of associated functions in plasmids of Rhizobium. Also, we have found that different strains of Rhizobium etli, share the localization on plasmids of some specific characteristics, independent of their geographic origin. Rhizobium strains from belonging to other species do not share this trait, even if they are able to nodulate bean.
We have described the regulatory mechanism of the conjugative transfer of a cryptic plasmid from R. etli (p42a).
We have studied the transfer capacity of symbiotic plasmid of R. etli CFN42. We have found two different mechanisms, one of them involves the cointegration of the symbiotic with another plasmid through a site specific recombination system. The resolution of the cointegrates sometimes leads to the generation of new symbiotic plasmids.

Present research position

Associate professor, Center of Genomic Sciences, National Autonomous University of Mexico.

Undergraduate: 11 courses
Masters: 10 courses
Ph.D.: 7 courses.

* Gabino Barreda medal, for undergraduate studies, National Autonomous University of Mexico, 1980.
* Honorific Mention in Undergraduate professional exam. National Autonomous University of Mexico, 1980.
Honorific Mention in Masters professional exam. National Autonomous University of Mexico, 1982.
* Gabino Barreda medal, for Ph.D. studies, National Autonomous University of Mexico, 1990.
* Sor Juana Inés de la Cruz medal for research contributions, 2005.



Telephone: (777) 3291691


2017Cubillas, C., Miranda-Sánchez, F., González-Sánchez, A., Elizalde, J.P., Vinuesa, P., Brom, S., García-de los Santos, A. (2017) A comprehensive phylogenetic analysis of copper transporting P-1B ATPases from bacteria of the Rhizobiales order uncovers multiplicity, diversity and novel taxonomic subtypes MICROBIOLOGYOPEN 6(4):e00452- [doi:10.1002/mbo3.452]28217917
2017Alfredo Banuelos-Vazquez, Luis, Torres Tejerizo, Gonzalo, Brom, Susana (2017) Regulation of conjugative transfer of plasmids and integrative conjugative elements Plasmid 91():82-89 [doi:10.1016/j.plasmid.2017.04.002]28438469
2015Torres Tejerizo, G., Bañuelos, L.A., Cervantes, L., Gaytán, P., Pistorio, M., Romero, D., Brom, S. (2015) Development of molecular tools to monitor conjugative transfer in Rhizobia JOURNAL OF MICROBIOLOGICAL METHODS 117():155-163 [doi:10.1016/j.mimet.2015.08.005]26272377
2014Tejerizo, G.T., Pistorio, M., Althabegoiti, M.J., Cervantes de la Luz, Laura, Wibberg, D., Schluter, A., Puhler, A., Lagares, A., Romero Camarena, David René, Brom Klanner, Susana (2014) Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background Fems Microbiology Ecology 88(3):565-578 [doi:10.1111/1574-6941.12325]24646299
2013Hernández Tamayo, Rogelio, Sohlenkamp, Christian, Puente, J.L., Brom Klanner, Susana, Romero Camarena, David René (2013) Characterization of IntA, a Bidirectional Site-Specific Recombinase Required for Conjugative Transfer of the Symbiotic Plasmid of Rhizobium etli CFN42 Journal Of Bacteriology 195(20):4668-4677 [doi:10.1128/JB.00714-13]23935046
2012Tejerizo, G.T., Lozano, L, Gonzalez, V, Bustos, P, Romero, D, Brom, S (2012) Draft Genome Sequence of the Bean-Nodulating Sinorhizobium fredii Strain GR64 Journal Of Bacteriology 194(24):6978-6978 [doi:10.1128/JB.01882-12]23209231
2011Landeta Escamilla, Cristina, Dávalos Rodríguez, Araceli Elvira, Cevallos Gaos, Miguel Ángel Carlos, Geiger, Otto, Brom Klanner, Susana, Romero Camarena, David René (2011) Plasmids with a Chromosome-Like Role in Rhizobia Journal Of Bacteriology 193(6):1317-1326 [doi:10.1128/JB.01184-10]21217003
2008Sepulveda, E., Perez-Mendoza, D., Ramirez-Romero, M. A., Soto, M. J., Lopez-Lara, I. M., Geiger, O., Sanjuan, J., Brom, S., Romero, D. (2008) Modulation of conjugative activity of rhizobial symbiotic plasmids by repression and transcriptional interference FEBS JOURNAL 275(1):49-49
2008Sepulveda, Edgardo, Perez-Mendoza, Daniel, Ramirez-Romero, Miguel A., Soto, Maria J., Lopez-Lara, Isabel M., Geiger, Otto, Sanjuan, Juan, Brom, Susana, Romero, David (2008) Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli Journal Of Bacteriology 190(12):4189-4197 [doi:10.1128/JB.00041-08]18424522
2008Garcfa-de los Santos, Alejandro, Lopez, Erika, Cubillas, Ciro A., Noel, K. Dale, Brom, Susana, Romero, David (2008) Requirement of a plasmid-encoded catalase for survival of Rhizobium etli CFN42 in a polyphenol-rich environment Applied And Environmental Microbiology 74(8):2398-2403 [doi:10.1128/AEM.02457-07]18310436
2007Brom, Susana, Garcia-De Los Santos, Alejandro, Romero, Laura Cervantes David (2007) Conjugative transfer of Rhizobium plasmids. Plasmid 57(2):220-220
2007Romero, David, Garcia-De Los Santos, Alejandro, Brom, Susana (2007) Essential plasmids in Rhizobium. Plasmid 57(2):227-227
2007Sepulveda, Edgardo, Perez-Mendoza, Daniel, Munoz, Socorro, Ramirez-Romero, Miguel A., Soto, Maria Jose, Cervantes, Laura, Herrera-Cervera, Jose A., Lopez-Lara, Isabel Sabel, Geiger, Otto, Sanuan, Juan, Brom, Susana, Romero, David (2007) Regulation of conjugal transfer of the Rhizobium etli Sym plasmid. Plasmid 57(2):220-221
2005Perez-Mendoza, D, Sepulveda, E, Pando, V, Munoz, S, Nogales, J, Olivares, J, Soto, MJ, Herrera-Cervera, JA, Romero, D, Brom, S, Sanjuan, J (2005) Identification of the rctA gene, which is required for repression of conjugative transfer of rhizobial symbiotic megaplasmids Journal Of Bacteriology 187(21):7341-7350 [doi:10.1128/JB.187.21.7341-7350.2005]16237017
2004Brom Klanner, Susana, Girard Cuesy, María de Lourdes, Tun Garrido, Cristina Soleda, García de los Santos, Alejandro, Bustos Arcos, Virginia Patricia, González Zúñiga, Víctor Manuel, Romero Camarena, David René (2004) Transfer of the symbiotic plasmid of Rhizobium etli CFN42 requires cointegration with p42a, which may be mediated by site-specific recombination Journal Of Bacteriology 186(22):7538-7548 [doi:10.1128/JB.186.22.7538-7548.2004]15516565
2004Sepulveda, Edgardo, Perez-Mendoza, Daniel, Munoz, Socorro, Ramirez-Romero, Miguel A., Soto, Maria Jose, Cervantes, Laura, Herrera-Cervera, Jose A., Lopez-Lara, Isabel Sabel, Geiger, Otto, Sanuan, Juan, Brom, Susana, Romero, David (2004) Identification of functional mob regions in Rhizobium etli: Evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d Journal Of Bacteriology 186(17):5753-5761 [doi:10.1128/JB.186.17.5753-5761.2004]15317780
2003Vargas, MD, Encarnacion, S, Davalos, A, Reyes-Perez, A, Mora, Y, Garcia-de los Santos, A, Brom, S, Moral, J (2003) Only one catalase, katG, is detectable in Rhizobium etli, and is encoded along with the regulator OxyR on a plasmid replicon MICROBIOLOGY-SGM 149(5):1165-1176 [doi:10.1099/mic.0.25909-0]12724378
2003Tun-Garrido, C, Bustos, P, Gonzalez, V, Brom, S (2003) Conjugative transfer of p42a from Rhizobium etli CFN42, which is required for mobilization of the symbiotic plasmid, is regulated by quorum sensing Journal Of Bacteriology 185(5):1681-1692 [doi:10.1128/JB.185.5.1681-1692.2003]12591886
2002Garcia-de los Santos, A, Morales, A, Baldoma, L, Clark, SRD, Brom, S, Yost, CK, Hernandez-Lucas, I, Aguilar, J, Hynes, MF (2002) The glcB locus of Rhizobium leguminosarum VF39 encodes an arabinose-inducible malate synthase Canadian Journal Of Microbiology 48(10):922-932 [doi:10.1139/W02-091]12489782
2002Cevallos, MA, Porta, H, Izquierdo, J, Tun-Garrido, C, Garcia-de-los-Santos, A, Davila, G, Brom, S (2002) Rhizobium etli CFN42 contains at least three plasmids of the repABC family: a structural and evolutionary analysis Plasmid 48(2):104-116 [doi:10.1016/S0147-619X(02)00119-1]12383728
2002Brom Klanner, Susana, Girard Cuesy, María de Lourdes, García de los Santos, Alejandro, Sanjuan-Pinilla, J.M., Olivares, J., Sanjuan, J. (2002) Conservation of plasmid-encoded traits among bean-nodulating Rhizobium species Applied And Environmental Microbiology 68(5):2555-2561 [doi:10.1128/AEM.68.5.2555-2561-2002]11976134
2000Girard Cuesy, María de Lourdes, Brom Klanner, Susana, Dávalos Rodríguez, Araceli Elvira, López, O., Soberón Chávez, Mario, Romero Camarena, David René (2000) Differential regulation of fixN-reiterated genes in Rhizobium etli by a novel fixL-fixK-cascade Molecular Plant-Microbe Interactions 13(12):1283-1292 [doi:10.1094/MPMI.2000.13.12.1283]11106020
2000Brom, S, Garcia-de los Santos, A, Cervantes, L, Palacios, R, Romero, D (2000) In Rhizobium etli symbiotic plasmid transfer, nodulation competitivity and cellular growth require interaction among different replicons Plasmid 44(1):34-43 [doi:10.1006/plas.2000.1469]10873525
1999Brom Klanner, Susana, Romero Camarena, David René (1999) Plasmid biology 98: international symposium on plasmid biology Plasmid 41(2):141-17710087219
1997GarciadelosSantos, A, Brom, S (1997) Characterization of two plasmid-borne lps beta loci of Rhizobium etli required for lipopolysaccharide synthesis and for optimal interaction with plants Molecular Plant-Microbe Interactions 10(7):891-902 [doi:10.1094/MPMI.1997.10.7.891]9304861
1996Santos, AGD, Brom, S, Romero, D (1996) Rhizobium plasmids in bacteria legume interactions World Journal Of Microbiology & Biotechnology 12(2):119-125 [doi:10.1007/BF00364676]24415159
1995ROMERO, D, MARTINEZSALAZAR, J, GIRARD, L, BROM, S, DAVILA, G, PALACIOS, R, FLORES, M, RODRIGUEZ, C (1995) DISCRETE AMPLIFIABLE REGIONS (AMPLICONS) IN THE SYMBIOTIC PLASMID OF Rhizobium-ETLI CFN42 Journal Of Bacteriology 177(4):973-980 [doi:10.1128/jb.177.4.973-980.1995]7860608
1993Flores López, Margarita, Brom Klanner, Susana, Stepkowsky, Tomász, Girard Cuesy, María de Lourdes, Dávila Ramos, José Guillermo, Romero Camarena, David René, Palacios de la Lama, Rafael (1993) GENE AMPLIFICATION IN Rhizobium - IDENTIFICATION AND INVIVO CLONING OF DISCRETE AMPLIFIABLE DNA REGIONS (AMPLICONS) FROM Rhizobium-leguminosarum BIOVAR phaseoli PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90(11):4932-4936 [doi:10.1073/pnas.90.11.4932]8506337
1992BROM, S, DELOSSANTOS, AG, STEPKOWSKI, T, FLORES, M, DAVILA, G, ROMERO, D, PALACIOS, R (1992) DIFFERENT PLASMIDS OF Rhizobium-leguminosarum BV phaseoli ARE REQUIRED FOR OPTIMAL SYMBIOTIC PERFORMANCE Journal Of Bacteriology 174(16):5183-5189 [doi:10.1128/jb.174.16.5183-5189.1992]1644746
1991GIRARD, MD, FLORES, M, BROM, S, ROMERO, D, PALACIOS, R, DAVILA, G (1991) STRUCTURAL COMPLEXITY OF THE SYMBIOTIC PLASMID OF Rhizobium-leguminosarum BV phaseoli Journal Of Bacteriology 173(8):2411-2419 [doi:10.1128/jb.173.8.2411-2419.1991]2013564
1991ROMERO, D, BROM, S, MARTINEZSALAZAR, J, GIRARD, MD, PALACIOS, R, DAVILA, G (1991) AMPLIFICATION AND DELETION OF A NOD-nif REGION IN THE SYMBIOTIC PLASMID OF Rhizobium-phaseoli Journal Of Bacteriology 173(8):2435-2441 [doi:10.1128/jb.173.8.2435-2441.1991]2013567
1991BROM, S, DELOSSANTOS, AG, DELOURDESGIRARD, M, DAVILA, G, PALACIOS, R, ROMERO, D (1991) HIGH-FREQUENCY REARRANGEMENTS IN Rhizobium-leguminosarum BV. phaseoli PLASMIDS Journal Of Bacteriology 173(3):1344-1346 [doi:10.1128/jb.173.3.1344-1346.1991]1991727
1988BROM, S, MARTINEZ, E, DAVILA, G, PALACIOS, R (1988) NARROW-HOST-RANGE AND BROAD-HOST-RANGE SYMBIOTIC PLASMIDS OF Rhizobium SPP STRAINS THAT NODULATE Phaseolus-VULGARIS Applied And Environmental Microbiology 54(5):1280-128316347637
1988MARTINEZ, E, FLORES, M, BROM, S, ROMERO, D, DAVILA, G, PALACIOS, R (1988) Rhizobium-phaseoli - A MOLECULAR-GENETICS VIEW PLANT AND SOIL 108(1):179-184 [doi:10.1007/BF02370113]
1987FLORES, M, GONZALEZ, V, BROM, S, MARTINEZ, E, PINERO, D, ROMERO, D, DAVILA, G, PALACIOS, R (1987) REITERATED DNA-SEQUENCES IN Rhizobium AND Agrobacterium SPP Journal Of Bacteriology 169(12):5782-5788 [doi:10.1128/jb.169.12.5782-5788.1987]3450286
2015López-Fuentes E, Torres-Tejerizo G, Cervantes L, Brom S (2015) Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors Frontiers in Microbiology 5():793-793 [doi:10.3389/fmicb.2014.00793]25642223
2016Hernández-Tamayo, R., Torres-Tejerizo, G., Brom, S., Romero, D. (2016) Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli Bmc Microbiology 16(1):133-133 [doi:10.1186/s12866-016-0755-y]27357704
2011Cervantes de la Luz, Laura, Bustos Arcos, Virginia Patricia, Girard Cuesy, María de Lourdes, Santamaria Gutiérrez, Rosa Isela, Dávila Ramos, José Guillermo, Vinuesa Fleischmann, Pablo, Romero Camarena, David René, Brom Klanner, Susana (2011) The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain Bmc Microbiology 11(1):1-11 [doi:10.1186/1471-2180-11-149]21702991