Michael Frederick Dunn

Functional Genomics of Prokaryotes Researcher

Associate Professor

Semblance

Michael F. Dunn earned his Bachelors of Science in Biology from Delaware Valley University (Doylestown, PA) in 1985. He worked for two semesters as a student intern in the plant tissue culture laboratory of Dr. Robert Zacharias at the U. S. Department of Agriculture’s Eastern Regional Research Center (ERRC) (Philadelphia, PA) and returned to the ERRC in 1985 to work as a technician in the bacterial phytopathology lab under Dr. William Fett. He began his doctoral work with Dr. Arthur Karr in the Plant Pathology Department at the University of Missouri-Columbia in 1987 and was awarded his Ph.D in 1992 with a thesis entitled “Characterization of an extracellular polysaccharide depolymerase produced by Bradyrhizobium japonicum”. He began his post-doctoral research with Dr. Jamie Mora at the National Autonomous University of Mexico’s Nitrogen Fixation Research Center (CIFN) in 1992. Here he initially focused on studying carbon metabolism (Kreb’s cycle and anaplerotic reactions, the glyoxylate shunt, and polyhydroxybutyrate synthesis) in the nitrogen-fixing bacteria Rhizobium etli and Sinorhizobium meliloti. As an Associate Researcher (1994-96) he focused on biotin metabolism in R. etli and characterized its pyruvate carboxylase, an enzyme that is still used biochemical and structural model for this class of biotin-dependent enzyme. He was a founding member of the Mexican Proteomics Society and collaborated in the first proteomic studies carried out in Mexico during the early years of the 21st Century, studying global metabolic protein expression in R. etli. In 2004, the CIFN became the Center for Genomic Science (CCG) and he became a tenured Research Professor in 2006. He focused on the functional genomics of rhizobial carbon metabolism for the remainder of that decade. In 2009, he was invited to collaborate with the Drs. Graciela Huerta and Karina Navarro of the Colegio de la Frontera Sur, Unidad Tapachula to study antibiotics and degradative enzymes produced by newly isolated bacterial biocontrol agents. Around this time, his interest in rhizobial metabolism shifted from carbon to nitrogen, specifically to studying the genetics and biochemistry of ornithine and arginine synthesis in S. meliloti. His current research is aimed at defining the roles of bioactive compounds called polyamines, which are derived from ornithine in S. meliloti. He and his students have discovered key points in the regulation of ornithine and polyamine biosynthesis, and are currently extending their biochemical characterization of the polyamine biosynthesis pathways and determining the effect of these compounds on gene transcription.

Links

Scopus

Telephone: (777) 3139944
Email: mike@ccg.unam.mx

Publications

YearPublicationPMID
2018Becerra-Rivera, V.A., Bergström, E., Thomas-Oates, J., Dunn, M.F. (2018) Polyamines are required for normal growth in sinorhizobium meliloti MICROBIOLOGY-SGM 164(4):600-613 [doi:10.1099/mic.0.000615]29619919
2017Meneses, N., Taboada, H., Dunn, M.F., Vargas, M.d.C., Buchs, N., Heller, M., Encarnación, S. (2017) The naringenin-induced exoproteome of Rhizobium etli CE3 Archives Of Microbiology 199(5):737-755 [doi:10.1007/s00203-017-1351-8]28255691
2017Gamez-Reyes, Aurora, Becerra-Lobato, Noe, Augusto Ramirez-Trujillo, Jose, Martinez-Romero, Esperanza, Dunn, Michael F., Hernandez-Lucas, Ismael (2017) The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems ANNALS OF MICROBIOLOGY 67(3):263-273 [doi:10.1007/s13213-017-1257-3]
2016Augusto Ramirez-Trujillo, Jose, Dunn, Michael F., Suarez-Rodriguez, Ramon, Hernandez-Lucas, Ismael (2016) The Sinorhizobium meliloti glyoxylate cycle enzyme isocitrate lyase (AceA) is required for the utilization of poly-beta-hydroxybutyrate during carbon starvation ANNALS OF MICROBIOLOGY 66(2):921-924 [doi:10.1007/s13213-015-1131-0]
2016Moreno Castillo, Benjamin, Dunn, Michael F., Guillen Navarro, Karina, Holguin Melendez, Francisco, Hernandez Ortiz, Magdalena, Encarnacion Guevara, Sergio, Huerta Palacios, Graciela (2016) Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella fijiensis Morelet World Journal Of Microbiology & Biotechnology 32(3):44- [doi:10.1007/s11274-015-1993-0]26873555
2015Frederick Dunn, Michael (2015) Key roles of microsymbiont amino acid metabolism in rhizobia-legume interactions Critical Reviews in Microbiology 41(4):411-451 [doi:10.3109/1040841X.2013.856854]24601835
2015Hernandez, Victor M., Girard, Lourdes, Hernandez-Lucas, Ismael, Vazquez, Alejandra, Ortiz-Ortiz, Catalina, Diaz, Rafael, Dunn, Michael F. (2015) Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021 MICROBIOLOGY-SGM 161(8):1671-1682 [doi:10.1099/mic.0.000122]26271664
2015Ingrid Gutierrez-Roman, Martha, Holguin-Melendez, Francisco, Dunn, Michael F., Guillen-Navarro, Karina, Huerta-Palacios, Graciela (2015) Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis, causal agent of black Sigatoka in banana (Musa spp.) Biocontrol 60(4):565-572 [doi:10.1007/s10526-015-9655-6]
2014Gutiérrez-Roman, M.I., Dunn, Michael Frederick, Tinoco-Valencia, R., Holguin-Melendez, F., Huerta-Palacios, G., Guillen-Navarro, K. (2014) Potentiation of the synergistic activities of chitinases ChiA, ChiB and ChiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP) World Journal Of Microbiology & Biotechnology 30(1):33-42 [doi:10.1007/s11274-013-1421-2]23824666
2013Gutiérrez-Román, M. I., Dunn, Michael Frederick, Tinoco-Valencia, F., Holguín-Meléndez, K., Guillen Navarro, Griselda Karina, Huerta-Palacios, G. (2013) Antifungal activity of chitinases (ChiA, ChiC and ChiB) produced by Serratia marcescens CFFSUR-B2 against Mycosphaerella fijiensis causal agent of black sigatoka in banana (Musa spp.) Acta Phytopathol 43():96-
2012Gutiérrez-Roman, M.I., Holguin-Melendez, F., Bello-Mendoza, R., Guillén-Navarro, K., Dunn, Michael Frederick, Huerta-Palacios, G. (2012) Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens World Journal Of Microbiology & Biotechnology 28(1):145-153 [doi:10.1007/s11274-011-0803-6]22806790
2011Dunn, Michael F. (2011) Anaplerotic Function of Phosphoenolpyruvate Carboxylase in Bradyrhizobium japonicum USDA110 Current Microbiology 62(6):1782-1788 [doi:10.1007/s00284-011-9928-y]21479798
2009Dunn, M. F., Ramirez-Trujillo, J. A., Hernandez-Lucas, I. (2009) Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis MICROBIOLOGY-SGM 155(Pt 10):3166-3175 [doi:10.1099/mic.0.030858-0]19684068
2008Martinez-Aguilar, Lourdes, Diaz, Rafael, Pena-Cabriales, Juan Jose, Estrada-de los Santos, Paulina, Dunn, Michael F., Caballero-Mellado, Jesus (2008) Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species Applied And Environmental Microbiology 74(14):4574-4579 [doi:10.1128/AEM.00201-08]18502926
2008Vargas Lagunas, María del Carmen, Villalobos, M.A., Díaz Mendez, Rafael, Flores, M.A., Contreras Martínez, Sandra, Encarnación Guevara, Sergio Manuel, Pérez-Tejada Domínguez, Yolanda, Dunn, Michael Frederick, Girard Cuesy, María de Lourdes, Mora Celis, Jaime (2008) Comparative Analysis of ArgC protein expression in Rhizobium species Current Plant Science and Biotechnology. Agriculture Biological Nitrogen Fixation, Sustainable Agriculture and the Environment 42(0):239-240
2007Ramirez-Trujillo, J. A., Encarnacion, S., Salazar, E., de los Santos, A. Garcia, Dunn, M. F., Emerich, D. W., Calva, E., Hernandez-Lucas, I. (2007) Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcB Journal Of Bacteriology 189(16):5875-5884 [doi:10.1128/JB.00385-07]17526694
2005Guillen-Navarro, K, Araiza, G, Garcia-de los Santos, A, Mora, Y, Dunn, MF (2005) The Rhizobium etli bioMNY operon is involved in biotin transport Fems Microbiology Letters 250(2):209-219 [doi:10.1016/j.femsle.2005.07.020]16099603
2005Guillen-Navarro, K, Encarnacion, S, Dunn, MF (2005) Biotin biosynthesis, transport and utilization in rhizobia Fems Microbiology Letters 246(2):159-165 [doi:10.1016/j.femsle.2005.04.020]15899401
2004Dunn, MF, Araiza, G, Mora, J (2004) Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A MICROBIOLOGY-SGM 150(2):399-406 [doi:10.1099/mic.0.26779-0]14766918
2003Encarnacion, S, Guzman, Y, Dunn, MF, Hernandez, M, Vargas, MD, Mora, J (2003) Proteome analysis of aerobic and fermentative metabolism in Rhizobium etli CE3 Proteomics 3(6):1077-1085 [doi:10.1002/pmic.200300427]12833533
2002Encarnacion, S, del Vargas, M, Dunn, MF, Davalos, A, Mendoza, G, Mora, Y, Mora, J (2002) AniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli Journal Of Bacteriology 184(8):2287-2295 [doi:10.1128/JB.184.8.2287-2295.2002]11914361
2002Dunn, MF, Araiza, G, Encarnacion, S, del Vargas, M, Mora, J (2002) Effect of aniA (carbon flux regulator) and phaC (poly-beta-hydroxybutyrate synthase) mutations on pyruvate metabolism in Rhizobium etli Journal Of Bacteriology 184(8):2296-2299 [doi:10.1128/JB.184.8.2296-2299.2002]11914362
2001Dunn, MF, Araiza, G, Finan, TM (2001) Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021 Archives Of Microbiology 176(5):355-363 [doi:10.1007/s002030100336]11702077
1998Dunn, MF (1998) Tricarboxylic acid cycle and anaplerotic enzymes in rhizobia Fems Microbiology Reviews 22(2):105-123 [doi:10.1111/j.1574-6976.1998.tb00363.x]9729766
1997Dunn, MF, Araiza, G, Cevallos, MA, Mora, J (1997) Regulation of pyruvate carboxylase in Rhizobium etli Fems Microbiology Letters 157(2):301-306 [doi:10.1016/S0378-1097(97)00491-6]9435112
1996Dunn, MF, Encarnacion, S, Araiza, G, Vargas, MC, Davalos, A, Peralta, H, Mora, Y, Mora, J (1996) Pyruvate carboxylase from Rhizobium etli: Mutant characterization, nucleotide sequence, and physiological role Journal Of Bacteriology 178(20):5960-5970 [doi:10.1128/jb.178.20.5960-5970.1996]8830693
1995ENCARNACION, S, DUNN, M, WILLMS, K, MORA, J (1995) FERMENTATIVE AND AEROBIC METABOLISM IN RHIZOBIUM-ETLI Journal Of Bacteriology 177(11):3058-3066 [doi:10.1128/jb.177.11.3058-3066.1995]7768801