Michael Frederick Dunn

Functional Genomics of Prokaryotes Researcher

Associate Professor

Academic history

Michael F. Dunn earned his Bachelors of Science in Biology from Delaware Valley University (Doylestown, PA) in 1985. He worked for two semesters as a student intern in the plant tissue culture laboratory of Dr. Robert Zacharias at the U. S. Department of Agriculture’s Eastern Regional Research Center (ERRC) (Philadelphia, PA) and returned to the ERRC in 1985 to work as a technician in the bacterial phytopathology lab under Dr. William Fett. He began his doctoral work with Dr. Arthur Karr in the Plant Pathology Department at the University of Missouri-Columbia in 1987 and was awarded his Ph.D in 1992 with a thesis entitled “Characterization of an extracellular polysaccharide depolymerase produced by Bradyrhizobium japonicum“. He began his post-doctoral research with Dr. Jamie Mora at the National Autonomous University of Mexico’s Nitrogen Fixation Research Center (CIFN) in 1992. Here he initially focused on studying carbon metabolism (Kreb’s cycle and anaplerotic reactions, the glyoxylate shunt, and polyhydroxybutyrate synthesis) in the nitrogen-fixing bacteria Rhizobium etli and Sinorhizobium meliloti. As an Associate Researcher (1994-96) he focused on biotin metabolism in R. etli and characterized its pyruvate carboxylase, an enzyme that is still used biochemical and structural model for this class of biotin-dependent enzyme. He was a founding member of the Mexican Proteomics Society and collaborated in the first proteomic studies carried out in Mexico during the early years of the 21st Century, studying global metabolic protein expression in R. etli. In 2004, the CIFN became the Center for Genomic Science (CCG) and he became a tenured Research Professor in 2006. He focused on the functional genomics of rhizobial carbon metabolism for the remainder of that decade. In 2009, he was invited to collaborate with the Drs. Graciela Huerta and Karina Navarro of the Colegio de la Frontera Sur, Unidad Tapachula to study antibiotics and degradative enzymes produced by newly isolated bacterial biocontrol agents. Around this time, his interest in rhizobial metabolism shifted from carbon to nitrogen, specifically to studying the genetics and biochemistry of ornithine and arginine synthesis in S. meliloti. His current research is aimed at defining the roles of bioactive compounds called polyamines, which are derived from ornithine in S. meliloti. He and his students have discovered key points in the regulation of ornithine and polyamine biosynthesis, and are currently extending their biochemical characterization of the polyamine biosynthesis pathways and determining the effect of these compounds on gene transcription.

Links

OrcID
Scopus

Telephone: (777) 120 4773
Email: mike@ccg.unam.mx

Publications

YearPublicationPMID
2018Becerra-Rivera, V.A., Bergström, E., Thomas-Oates, J. and Dunn, MF. (2018). "Polyamines are required for normal growth in Sinorhizobium meliloti". MICROBIOLOGY-SGM. 164(4):600-613. [doi:10.1099/mic.0.000615]29619919
2017Meneses, N., Taboada, H, Dunn, MF, Vargas, MC, Buchs, N., Heller, M. and Encarnación, S. (2017). "The naringenin-induced exoproteome of Rhizobium etli CE3". Archives Of Microbiology. 199(5):737-755. [doi:10.1007/s00203-017-1351-8]28255691
2017Gamez-Reyes, Aurora, Becerra-Lobato, Noe, Augusto Ramirez-Trujillo, Jose, Martínez-Romero, E, Dunn, MF and Hernandez-Lucas, Ismael. (2017). "The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems". ANNALS OF MICROBIOLOGY. 67(3):263-273. [doi:10.1007/s13213-017-1257-3]
2017Dunn, MF. (2017). "Rhizobial Amino Acid Metabolism: Polyamine Biosynthesis and Functions". Handbook Of Microbial Metabolism Of Amino Acids. ():352-370.
2016Augusto Ramirez-Trujillo, Jose, Dunn, MF, Suarez-Rodriguez, Ramon and Hernandez-Lucas, Ismael. (2016). "The Sinorhizobium meliloti glyoxylate cycle enzyme isocitrate lyase (aceA) is required for the utilization of poly-ß-hydroxybutyrate during carbon starvation". ANNALS OF MICROBIOLOGY. 66(2):921-924. [doi:10.1007/s13213-015-1131-0]
2016Moreno Castillo, Benjamin, Dunn, MF, Guillen Navarro, Karina, Holguin Melendez, Francisco, Hérnandez, M, Encarnación, S and Huerta Palacios, Graciela. (2016). "Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella Fijiensis Morelet". World Journal Of Microbiology & Biotechnology. 32(3):1-12. [doi:10.1007/s11274-015-1993-0]26873555
2015Dunn, MF. (2015). "Key roles of microsymbiont amino acid metabolism in Rhizobia-legume interactions". Critical Reviews in Microbiology. 41(4):411-451. [doi:10.3109/1040841X.2013.856854]24601835
2015Hernandez, Victor M., Girard, L, Hernandez-Lucas, Ismael, Vazquez, Alejandra, Ortiz-Ortiz, Catalina, Díaz-Mendez, R and Dunn, MF. (2015). "Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021". MICROBIOLOGY-SGM. 161(8):1671-1682. [doi:10.1099/mic.0.000122]26271664
2014Gutiérrez-Roman, M.I., Dunn, MF, Tinoco-Valencia, R., Holguin-Melendez, F., Huerta-Palacios, G. and Guillen-Navarro, K.. (2014). "Potentiation of the synergistic activities of chitinases chiA, chiB and chiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP)". World Journal Of Microbiology & Biotechnology. 30(1):33-42. [doi:10.1007/s11274-013-1421-2]23824666
2013Gutiérrez-Román, M. I., Dunn, MF, Tinoco-Valencia, F., Holguín-Meléndez, K., Guillen Navarro, Griselda Karina and Huerta-Palacios, G.. (2013). "Antifungal activity of chitinases (chiA, chiC and chiB) produced by Serratia marcescens CFFSUR-B2 against Mycosphaerella Fijiensis causal agent of black sigatoka in banana (Musa spp.)". Acta Phytopathol. 43():96-.
2012Gutiérrez-Roman, M.I., Holguin-Melendez, F., Bello-Mendoza, R., Guillén-Navarro, K., Dunn, MF and Huerta-Palacios, G.. (2012). "Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens". World Journal Of Microbiology & Biotechnology. 28(1):145-153. [doi:10.1007/s11274-011-0803-6]22806790
2011Dunn, MF. (2011). "Anaplerotic function of phosphoenolpyruvate carboxylase in Bradyrhizobium japonicum USDA110". Current Microbiology. 62(6):1782-1788. [doi:10.1007/s00284-011-9928-y]21479798
2008Martínez-Aguilar, L, Díaz-Mendez, R, Pena-Cabriales, Juan Jose, Estrada-de los Santos, Paulina, Dunn, MF and Caballero-Mellado, Jesus. (2008). "Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species". Applied And Environmental Microbiology. 74(14):4574-4579. [doi:10.1128/AEM.00201-08]18502926
2008Vargas, MC, Villalobos, M.A., Díaz-Mendez, R, Flores, M.A., Contreras-Martínez, S, Encarnación, S, Pérez-Tejada Domínguez, Yolanda, Dunn, MF, Girard, L and Mora, J. (2008). "Comparative Analysis of argC protein expression in Rhizobium species". Current Plant Science and Biotechnology. Agriculture Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. 42(0):239-240.
2007Ramirez-Trujillo, J. A., Encarnación, S, Salazar, E., García-de los Santos, A, Dunn, MF, Emerich, D. W., Calva, E. and Hernandez-Lucas, I.. (2007). "Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcB". Journal Of Bacteriology. 189(16):5875-5884. [doi:10.1128/JB.00385-07]17526694
2004Dunn, MF, Araiza, G and Mora, J. (2004). "Biochemical characterization of a Rhizobium etli monovalent cation-stimulated acyl-coenzyme A carboxylase with a high substrate specificity constant for propionyl-coenzyme A". MICROBIOLOGY-SGM. 150(2):399-406. [doi:10.1099/mic.0.26779-0]14766918
2003Encarnación, S, Guzman, Y, Dunn, MF, Hernandez, M, Vargas, MD and Mora, J. (2003). "Proteome analysis of aerobic and fermentative metabolism in Rhizobium etli CE3". Proteomics. 3(6):1077-1085. [doi:10.1002/pmic.200300427]12833533
2002Encarnación, S, Vargas, MC, Dunn, MF, Dávalos, A, Mendoza, G, Mora, Y and Mora, J. (2002). "aniA regulates reserve polymer accumulation and global protein expression in Rhizobium etli". Journal Of Bacteriology. 184(8):2287-2295. [doi:10.1128/JB.184.8.2287-2295.2002]11914361
2002Dunn, MF, Araiza, G, Encarnación, S, Vargas, MC and Mora, J. (2002). "Effect of aniA (carbon flux regulator) and phaC (poly-ß-hydroxybutyrate synthase) mutations on pyruvate metabolism in Rhizobium etli". Journal Of Bacteriology. 184(8):2296-2299. [doi:10.1128/JB.184.8.2296-2299.2002]11914362
2001Dunn, MF, Araiza, G and Finan, TM. (2001). "Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm1021". Archives Of Microbiology. 176(5):355-363. [doi:10.1007/s002030100336]11702077
1998Dunn, MF. (1998). "Tricarboxylic acid cycle and anaplerotic enzymes in Rhizobia". Fems Microbiology Reviews. 22(2):105-123. [doi:10.1016/S0168-6445(98)00010-2]9729766
1996Dunn, MF, Encarnación, S, Araiza, G, Vargas, MC, Dávalos, A, Peralta, H, Mora, Y and Mora, J. (1996). "Pyruvate carboxylase from Rhizobium etli: Mutant characterization, nucleotide sequence, and physiological role". Journal Of Bacteriology. 178(20):5960-5970. [doi:10.1128/jb.178.20.5960-5970.1996]8830693
1995Encarnación, S, Dunn, MF, WILLMS, K and Mora, J. (1995). "Fermentative and aerobic metabolism in Rhizobium etli". Journal Of Bacteriology. 177(11):3058-3066. [doi:10.1128/jb.177.11.3058-3066.1995]7768801
1997Dunn, MF, Araiza, G, Cevallos, MA and Mora, J. (1997). "Regulation of pyruvate carboxylase in Rhizobium etli". Fems Microbiology Letters. 157(2):301-306. [doi:10.1016/S0378-1097(97)00491-6]9435112
2018Taboada, H, Meneses N, Dunn, MF, Vargas, MC, Buchs N, Castro-Mondragón JA, Heller M and Encarnación, S. (2018). "Proteins in the periplasmic space and outer membrane vesicles of Rhizobium etli CE3 grown in minimal medium are largely distinct and change with growth phase.". MICROBIOLOGY-SGM. ():-. [doi:10.1099/mic.0.000720]30358529
2005Guillen-Navarro, K, Encarnación, S and Dunn, MF. (2005). "Biotin biosynthesis, transport and utilization in Rhizobia". Fems Microbiology Letters. 246(2):159-165. [doi:10.1016/j.femsle.2005.04.020]15899401
2005Guillen-Navarro, K, Araiza, G, García-de los Santos, A, Mora, Y and Dunn, MF. (2005). "The Rhizobium etli bioMNY operon is involved in biotin transport". Fems Microbiology Letters. 250(2):209-219. [doi:10.1016/j.femsle.2005.07.020]16099603
2009Dunn, MF, Ramirez-Trujillo, J. A. and Hernandez-Lucas, I.. (2009). "Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis". MICROBIOLOGY-SGM. 155(10):3166-3175. [doi:10.1099/mic.0.030858-0]19684068
2015Ingrid Gutierrez-Roman, Martha, Holguin-Melendez, Francisco, Dunn, MF, Guillen-Navarro, Karina and Huerta-Palacios, Graciela. (2015). "Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella Fijiensis, causal agent of black Sigatoka in banana (Musa spp.)". Biocontrol. 60(4):565-572. [doi:10.1007/s10526-015-9655-6]
1970Rosas-Pérez, T and Dunn, MF. (1970). "The Symbiome of Llaveia Cochineals (Hemiptera: Coccoidea: Monophlebidae) Includes a Gammaproteobacterial Cosymbiont Sodalis TME1 and the Known Candidatus Walczuchella monophlebidarum". Insect Physiology and Ecology. ():115-134. [doi:10.5772/66442]