Isabel María López Lara

Ecological Genomics Researcher

Associate Professor

Academic history

Isabel M. López-Lara studied biology at the University of Granada, in Granada, Spain. She received her doctoral title from the University of Granada in 1992 with the work “Genetic and biochemical studies of surface polysaccharides of Rhizobium sp. GRH2″ that she carried out in the Estación Experimental del Zaidín, Granada, Spanish Council for Scientific Research (CSIC) under the supervision of Nicolás Toro García. From 1993 to 1995, she did postdoctoral work in Leiden (The Netherlands) at the Institute of Molecular Plant Sciences with Herman P. Spaink on the “Molecular basis of the Rhizobium-legume interaction” with fellowships from the Spanish Ministry and the European Community. Subsequently, as an EMBO fellow, she did postdoctoral work in the Department of Biotechnology at the Technical University of Berlin, Germany, working on “Lipid biosynthesis in Rhizobia” with Otto Geiger. She currently holds the position of Assistant Professor (Investigador Titular B) at the Center for Genomic Sciences (formerly the Research Center for Nitrogen Fixation) at the National Autonomous University of Mexico (Cuernavaca) which she joined in 1999. Presently she studies the metabolism of fatty acids in bacteria, including their biosynthesis, modifications to form signal molecules, and transfer of fatty acids during the formation of membrane lipids. She was Associate Editor for the Journal Molecular Plant-Microbe Interactions for the period 2004-2006.

Links

OrcID
Scopus

Telephone: (777) 3131697
Email: isabel@ccg.unam.mx

Publications

YearPublicationPMID
2019Luis Folch-Mallol, J, Zarate, A, Sanchez-Reyes, A, Lopez-Lara, I. (2019). "Expression, purification, and characterization of a metagenomic thioesterase from activated sludge involved in the degradation of acylCoA-derivatives". PROTEIN EXPRES PURIF. 159():49-52. [doi:10.1016/j.pep.2019.03.008]30905871
2018López-Lara, I, Nogales, J, Pech-Canul, Á, Calatrava-Morales, N, Bernabéu-Roda, L, Durán, P, Cuéllar, V, Olivares, J, Alvarez, L, Palenzuela-Bretones, D, Romero, M, Heeb, S, Cámara, M, Geiger, O, Soto, M. (2018). "2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant–bacteria interactions". Environmental Microbiology. 20(6):2049-2065. [doi:10.1111/1462-2920.14083]29488306
2018López Lara, I, Geiger, O. (2018). "Formation of fatty acids". Biogenesis of Fatty Acids, Lipids and Membranes. ():44-55. [doi:10.1007/978-3-319-50430-8_5]
2018Geiger, O, Sohlenkamp, C, López Lara, I. (2018). "Formation of bacterial glycerol-based membrane lipids: pathways, enzymes, reactions.". Biogenesis of Fatty Acids, Lipids and Membranes. ():87-107. [doi:10.1007/978-3-319-50430-8_8]
2018Geiger, O, Padilla Gómez, J, López Lara, I. (2018). "Bacterial sphingolipids and sulfonolipids.". Biogenesis of Fatty Acids, Lipids and Membranes. ():273-289. [doi:10.1007/978-3-319-50430-8_12]
2018Sahonero Canavesi, D, López Lara, I, Geiger, O. (2018). "Membrane lipid degradation and lipid cycles in microbes.". Aerobic Utilization of Hydrocarbons, Oils and Lipids. ():1-24. [doi:10.1007/978-3-319-39782-5_38-1]
2018López Lara, I, Soto Misffut, M. (2018). "Fatty acid synthesis and regulation". Biogenesis of Fatty Acids, Lipids and Membranes. ():3-23. [doi:10.1007/978-3-319-50430-8_2]
2017López-Lara, I, Geiger, O. (2017). "Bacterial lipid diversity". Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1862(11):1287-1299. [doi:10.1016/j.bbalip.2016.10.007]27760387
2017Angel Vences-Guzman, M, Goetting-Minesky, M, Guan, Z, Castillo-Ramirez, S, America Cordoba-Castro, L, Lopez-Lara, I, Geiger, O, Sohlenkamp, C, Fenno, J. (2017). "1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway". Molecular Microbiology. 103(5):896-912. [doi:10.1111/mmi.13596]28009086
2017Soto, M, Calatrava Morales, N, López Lara, I. (2017). "Functional roles of non-membrane lipids in bacterial signaling". Biogenesis of Fatty Acids, Lipids and Membranes. ():-. [doi:10.1007/978-3-319-43676-0_16-2]
2015Escobedo-Hinojosa, W, Vences-Guzmán, M, Schubotz, F, Sandoval-Calderón, M, Summons, R, López-Lara, I, Geiger, O, Sohlenkamp, C. (2015). "OlsG (Sinac-1600) Is an ornithine lipid N-Methyltransferase from the planctomycete Singulisphaera acidiphila". JOURNAL OF BIOLOGICAL CHEMISTRY. 290(24):15102-15111. [doi:10.1074/jbc.M115.639575]25925947
2015Sahonero-Canavesi, D, Sohlenkamp, C, Sandoval-Calderón, M, Lamsa, A, Pogliano, K, López-Lara, I, Geiger, O. (2015). "Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase". Environmental Microbiology. 17(9):3391-3406. [doi:10.1111/1462-2920.12814]25711932
2013Geiger, O, Lopez-Lara, I, Sohlenkamp, C. (2013). "Phosphatidylcholine biosynthesis and function in bacteria". Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1831(3):503-513. [doi:10.1016/j.bbalip.2012.08.009]22922101
2012Estrada De Los Santos, P, Martínez Aguilar, L, López Lara, I, Caballero Mellado, J. (2012). "Cupriavidus Alkaliphilus sp. nov., a new species associated with agricultural plants that grow in alkaline soils". Systematic And Applied Microbiology. 35(5):310-314. [doi:10.1016/j.syapm.2012.05.005]22738764
2011Pech Canul, A, Nogales, J, Miranda-Molina, A, Álvarez, L, Geiger, O, Soto, M, López Lara, I. (2011). "FadD is required for utilization of endogenous fatty acids released from membrane lipids". Journal Of Bacteriology. 193(22):6295-6304. [doi:10.1128/JB.05450-11]21926226
2011Vences Guzmán, M, Guan, Z, Ormeño Orrillo, E, González Silva, N, López Lara, I, Martínez Romero, M, Geiger, O, Sohlenkamp, C. (2011). "Hydroxylated ornithine lipids increase stress tolerance in Rhizobium tropici CIAT899". Molecular Microbiology. 79(6):1496-1514. [doi:10.1111/j.1365-2958.2011.07535.x]21205018
2010Medeot, D, Sohlenkamp, C, Dardanelli, M, Geiger, O, Garcia de Lema, M, Lopez-Lara, I. (2010). "Phosphatidylcholine levels of peanut-nodulating Bradyrhizobium sp. SEMIA 6144 affect cell size and motility". Fems Microbiology Letters. 303(2):123-131. [doi:10.1111/j.1574-6968.2009.01873.x]20030724
2010Geiger, O, Sohlenkamp, C, López Lara, I. (2010). "Formation of bacterial membrane lipids: pathways, enzymes, reactions". Handbook of Hydrocarbon and Lipid Microbiology Hydrocarbons, Oils, and Lipids: Diversity, Properties and Formation. ():396-405.
2010López Lara, I, Geiger, O. (2010). "Formation of fatty acids". Handbook of Hydrocarbon and Lipid Microbiology Hydrocarbons, Oils, and Lipids: Diversity, Properties and Formation. ():385-394.
2010Geiger, O, Gonzalez-Silva, N, Lopez-Lara, I, Sohlenkamp, C. (2010). "Amino acid-containing membrane lipids in bacteria". Progress In Lipid Research. 49(1):46-60. [doi:10.1016/j.plipres.2009.08.002]19703488
2008Sepulveda, E, Perez-Mendoza, D, Ramirez-Romero, M, Soto, M, Lopez-Lara, I, Geiger, O, Sanjuan, J, Brom, S, Romero, D. (2008). "Modulation of conjugative activity of rhizobial symbiotic plasmids by repression and transcriptional interference". FEBS JOURNAL. 275(1):49-49.
2008Sepulveda, E, Perez-Mendoza, D, Ramirez-Romero, M, Soto, M, Lopez-Lara, I, Geiger, O, Sanjuan, J, Brom, S, Romero, D. (2008). "Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli". Journal Of Bacteriology. 190(12):4189-4197. [doi:10.1128/JB.00041-08]18424522
2008Geiger, O, Lopez-Lara, I, Sohlenkamp, C. (2008). "ALTERED FORMATIONS OF SPECIAL EUBACTERIAL MEMBRANE LIPIDS AFFECT BACTERIAL INTERACTIONS WITH EUKARYOTIC HOSTS". Biological Nitrogen Fixation: Towards Poverty Alleviation Through Sustainable Agriculture. 42():245-246.
2008Geiger, O, López Lara, I, Sohlenkamp, C. (2008). "Altered formations of special eubacterial membrane lipids affect bacterial interactions with eukaryotic hosts". Current Plant Science and Biotechnology. Agriculture Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. 42(0):245-246.
2007Sepulveda, E, Perez-Mendoza, D, Munoz, S, Ramirez-Romero, M, Soto, M, Cervantes, L, Herrera-Cervera, J, Lopez-Lara, I, Geiger, O, Sanuan, J, Brom, S, Romero, D. (2007). "Regulation of conjugal transfer of the Rhizobium etli Sym plasmid.". Plasmid. 57(2):220-221.
2007Medeot, D, Sohlenkamp, C, Geiger, O, García, M, López Lara, I. (2007). "Phosphatidycholine fuction in rhizobial strains nodulating peanut: genes and enzymatic activites". Biocell. 31():134-.
2006Geiger, O, Sohlenkamp, C, López Lara, I. (2006). "Function of rhizobial membrane lipids for the symbiosis with legumes". Proceedings of the 12th International Congress on Molecular Plant-Microbe Interactions. 5():132-137.
2006Sánchez, F, Quinto, C, López Lara, I, Geiger, O. (2006). "Biology of Plant-Microbe Interactions". Proceedings of the 12th International Congress on Molecular Plant-Microbe Interactions. 5():1-717.
2006López Lara, I, Sohlenkamp, C, Geiger, O. (2006). "The post-genomic era - What comes next?". Revista Latinoamericana De Microbiologia. 48(1):44-45. 17357573
2005Lopez-Lara, I, Gao, J, Soto, M, Solares-Perez, A, Weissenmayer, B, Sohlenkamp, C, Verroios, G, Thomas-Oates, J, Geiger, O. (2005). "Phosphorus-free membrane lipids of Sinorhizobium meliloti are not required for the symbiosis with alfalfa but contribute to increased cell yields under phosphorus-limiting conditions of growth". Molecular Plant-Microbe Interactions. 18(9):973-982. [doi:10.1094/MPMI-18-0973]16167767
2004Geiger, O, Martínez Morales, F, Sohlenkamp, C, López Lara, I. (2004). "ROLE OF BACTERIAL PHOSPHATIDYLCHOLINE IN SYMBIOTIC AND PATHOGENIC INTERACTIONS". Biology of Molecular Plant-Microbe Interactions. ():423-426.
2004Sepulveda, E, Perez-Mendoza, D, Munoz, S, Ramirez-Romero, M, Soto, M, Cervantes, L, Herrera-Cervera, J, Lopez-Lara, I, Geiger, O, Sanuan, J, Brom, S, Romero, D. (2004). "Identification of functional mob regions in Rhizobium etli: Evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d". Journal Of Bacteriology. 186(17):5753-5761. [doi:10.1128/JB.186.17.5753-5761.2004]15317780
2004Gao, J, Weissenmayer, B, Taylor, A, Thomas-Oates, J, Lopez-Lara, I, Geiger, O. (2004). "Identification of a gene required for the formation of lyso-ornithine lipid, an intermediate in the biosynthesis of ornithine-containing lipids". Molecular Microbiology. 53(6):1757-1770. [doi:10.1111/j.1365-2958.2004.04240.x]15341653
2003Martinez-Morales, F, Schobert, M, Lopez-Lara, I, Geiger, O. (2003). "Pathways for phosphatidylcholine biosynthesis in bacteria". MICROBIOLOGY-SGM. 149(12):3461-3471. [doi:10.1099/mic.0.26522-0]14663079
2003Lopez-Lara, I, Sohlenkamp, C, Geiger, O. (2003). "Membrane lipids in plant-associated bacteria: Their biosyntheses and possible functions". Molecular Plant-Microbe Interactions. 16(7):567-579. [doi:10.1094/MPMI.2003.16.7.567]12848422
2003Sohlenkamp, C, Lopez-Lara, I, Geiger, O. (2003). "Biosynthesis of phosphatidylcholine in bacteria". Progress In Lipid Research. 42(2):115-162. [doi:10.1016/S0163-7827(02)00050-4]12547654
2002Geiger, O, López Lara, I. (2002). "Rhizobial acyl carrier proteins and their roles in the formation of bacterial cell-surface components that are required for the development of nitrogen-fixing root nodules on legume hosts". Fems Microbiology Letters. 208(2):153-162. [doi:10.1016/S0378-1097(02)00478-0]11959430
2001Lopez-Lara, I, Geiger, O. (2001). "The nodulation protein NodG shows the enzymatic activity of an 3-oxoacyl-acyl carrier protein reductase". Molecular Plant-Microbe Interactions. 14(3):349-357. [doi:10.1094/MPMI.2001.14.3.349]11277432
2001Lopez-Lara, I, Geiger, O. (2001). "Novel pathway for phosphatidylcholine biosynthesis in bacteria associated with eukaryotes". JOURNAL OF BIOTECHNOLOGY. 91(2-3):211-221. [doi:10.1016/S0168-1656(01)00331-5]11566392
2001Wang, E, Martínez Romero, J, López Lara, I. (2001). "Rhizobium y su destacada simbiosis con plantas". Microbios en Línea. ():-.
2000López-Lara, I, Geiger, O. (2000). "Expression and purification of four different rhizobial acyl carrier proteins". MICROBIOLOGY-SGM. 146(4):839-849. [doi:10.1099/00221287-146-4-839]10784042
1998Olsthoorn, M, López Lara, I, Petersen, B, Bock, K, Haverkamp, J, Spaink, H, Thomas Oates, J. (1998). "Novel branched Nod factor structure results from -(1 3) fucosyl transferase activity: the major lipo-chitin oligosaccharides from Mesorhizobium loti strain NZP2213 bear an -(1 3) fucosyl subtituent on a nonterminal backbone residue". Biochemistry. 37(25):9024-9032.
1997Spaink, H, Bakkers, J, Bladergroen, M, Bloemberg, G, Dandal, I, Díaz, C, Blok Tip, L, Gisel, A, Harteveld, M, López Lara, I, Kafetzopoulos, D, Kamst, E, Kijne, J, Meijer, D, Lugtenberg, B, Ovtsyna, A, Potrykus, I, Quaedvlieg, N, Quinto, C, Ritsema, T, Sautter, C, Schlaman, H, Thomas Oates, J, van Boom, J, van der Drift, K, van der Marel, G, van Leeuwen, S, Veldhuis, A, Wijfjes, A. (1997). "The molecular basis of host specificity of Rhizobia". Biology of plant-microbe interactions: Proceedings of the 8th. International Symposium on Molecular Plant-Microbr Interactions, Knoxville, Tennessee, july 14-19, 1996. 1():299-306.
1996Thomas-Oates, J, van den Berg, J, Bloemberg, G, van der Drift, K, Geiger, O, López Lara, I, Rademaker, G, Spaink, H. (1996). "Structural determination and biosynthetic studies of the rhizobial Nod metabolites: the lipo-chitin oligosaccharides". Mass spectrometry in the Biological Sciences. ():385-401. [doi:10.1007/978-1-4612-0229-5]
1996Folch Mallol, J, Marroqui, S, Sousa, C, Manyani, H, López Lara, I, van der Drift, K, Haverkamp, J, Quinto, C, Gil Serrano, A, Thomas Oates, J, Spaink, H, Megías, M. (1996). "Characterization of Rhizobium tropici CIAT899 nodulation factors: the role of nodH and nodPQ in their sulphation". Molecular Plant-Microbe Interactions. 9(3):151-163.
1995López-Lara, I, van Der Drift, K, van Brussel, A, Haverkamp, J, Lugtenberg, B, Thomas-Oates, J, Spaink, H. (1995). "Induction of nodule primordia on Phaseolus and Acacia by lipo-chitin oligosaccharide nodulation signals from broad-host-range Rhizobium strain GRH2". PLANT MOLECULAR BIOLOGY. 29(3):465-477. [doi:10.1007/BF00020978]8534846
1995Cárdenas, L, Domínguez, J, Quinto, C, López-Lara, I, Lugtenberg, B, Spaink, H, Rademaker, G, Haverkamp, J, Thomas-Oates, J. (1995). "Isolation, chemical structures and biological activity of the lipo-chitin oligosaccharide nodulation signals from Rhizobium etli". PLANT MOLECULAR BIOLOGY. 29(3):453-464. [doi:10.1007/BF00020977]8534845
1995López Lara, I, Orgambide, O, Dazzo, F, Olivares, J, Toro, N. (1995). "Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain GRH2: Major requirement of lipopolysaccharide for successful invasion of Acacia nodules and host range determination". MICROBIOLOGY-SGM. 141(3):573-581. [doi:10.1099/13500872-141-3-573]7711895
1995López Lara, I, van den Berg, J, Thomas-Oates, J, Glushka, J, Lugtenberg, B, Spaink, H. (1995). "Structural identification of the iipo-chitin oligosaccharide nodulation signals of Rhizobium loti". Molecular Microbiology. 15(4):627-638. [doi:10.1111/j.1365-2958.1995.tb02372.x]7783635
1994Spaink, H, Bloemberg, G, Wijfjes, A, Ritsema, T, López Lara, I, Geiger, O, Harteveld, M, Kafetzopoulos, D, van Brussel, A, Kijne, J, Lugtenberg, B, van der Drift, K, Thomas-Oates, J, Potrykus, I, Sautter, C. (1994). "The molecular basis of host specificity in the Rhizobium leguminosarum-plant interaction". Advances in Molecular Genetics of Plant-Microbe Interactions. Vol. 3 Proceedings of the 7th International Symposium on Molecular Plant-Microbe Interactions, Edinburgh, U.K., June 1994. 3():91-98. [doi:10.1007/978-94-011-0177-6]
1994Soto, M, Zorzano, A, Garcia-Rodriguez, F, Mercado-Blanco, J, Lopez-Lara, I, Olivares, J, Toro, N. (1994). "Identification of a novel Rhizobium meliloti nodulation efficiency nfe gene homolog of Agrobacterium ornithine cyclodeaminase". Molecular Plant-Microbe Interactions. 7(6):703-707. [doi:10.1094/MPMI-7-0703]7873778
1993López Lara, I, Orgambide, G, Dazzo, F, Olivares, J, Toro, N. (1993). "Characterization and symbiotic importance of acidic extracellular polysaccharides of Rhizobium sp. strain GRH2 isolated from Acacia nodules". Journal Of Bacteriology. 175(10):2826-2832. [doi:10.1128/jb.175.10.2826-2832.1993]8491702
1992Soto, M, Lepek, V, López-Lara, I, Olivares, J, Toro, N. (1992). "Characterization of a Rhizobium meliloti ndvB Mutant and a Symbiotic Revertant that Regains Wild-Type Properties". Molecular Plant-Microbe Interactions. 5(4):288-293. [doi:10.1094/MPMI-5-288]
1991López Lara, I, Gálvez, A, Martínez Bueno, M, Maqueda, M, Valdivia, E. (1991). "Purification, characterization and biological effects of a second bacteriocin from Enterococcus faecalis subsp. liquefaciens S-48 and its mutant strain B-48-28". Canadian Journal Of Microbiology. 37(10):769-774.
1990Lara, I, Martínez-Bueno, M, Gálvez, A, Maqueda, M, Valdivia, E. (1990). "Induction of inhibitory agent produced by Enterococcus faecalis". FOLIA MICROBIOL. 35(2):124-129. [doi:10.1007/BF02820768]2116373
2009Laura Ramos-Vega, A, Davila-Martinez, Y, Sohlenkamp, C, Contreras-Martinez, S, Encarnacion, S, Geiger, O, Lopez-Lara, I. (2009). "SMb20651 is another acyl carrier protein from Sinorhizobium meliloti". MICROBIOLOGY-SGM. 155(1):257-267. [doi:10.1099/mic.0.022079-0]19118366
1997Quinto, C, Wijfjes, A, Bloemberg, G, Blok-Tip, L, López-Lara, I, Lugtenberg, B, Thomas-Oates, J, Spaink, H. (1997). "Bacterial nodulation protein nodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin". PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94(9):4336-4341. [doi:10.1073/pnas.94.9.4336]9113990
2000Castillo, P, Marquez, J, Rubluo, A, Hernandez, G, Lara, I. (2000). "Plant regeneration from callus and suspension cultures of Valeriana edulis ssp procera via simultaneous organogenesis and somatic embryogenesis". Plant Science. 151(2):115-119. [doi:10.1016/S0168-9452(99)00203-4]10808067
2000Sohlenkamp, C, de Rudder, K, Rohrs, V, Lopez-Lara, I, Geiger, O. (2000). "Cloning and characterization of the gene for phosphatidylcholine synthase". JOURNAL OF BIOLOGICAL CHEMISTRY. 275(25):18919-18925. [doi:10.1074/jbc.M000844200]10858449
2010Zavaleta-Pastor, M, Sohlenkamp, C, Gao, J, Guan, Z, Zaheer, R, Finan, T, Raetz, C, Lopez-Lara, I, Geiger, O. (2010). "Sinorhizobium meliloti phospholipase C required for lipid remodeling during phosphorus limitation". PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 107(1):302-307. [doi:10.1073/pnas.0912930107]20018679
2002Weissenmayer, B, Gao, J, Lopez-Lara, I, Geiger, O. (2002). "Identification of a gene required for the biosynthesis of ornithine-derived lipids". Molecular Microbiology. 45(3):721-733. [doi:10.1046/j.1365-2958.2002.03043.x]12139618
2004Geiger, O, Sohlenkamp, C, Martinez-Morales, F, Gao, J, Zavaleta-Pastor, M, Lopez-Lara, I. (2004). "Regulation of membrane lipid biosynthesis in eubacteria". FASEB JOURNAL. 18(8, S):3-3.
2016Sahonero-Canavesi, D, Zavaleta-Pastor, M, Martinez-Aguilar, L, Lopez-Lara, I, Geiger, O. (2016). "Defining substrate specificities for lipase and phospholipase candidates". JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2016(117):e54613-. [doi:10.3791/54613]27911408
2011González Silva, N, López Lara, I, Reyes-Lamothe, R, Taylor, A, Sumpton, D, Thomas-Oates, J, Geiger, O. (2011). "The dioxygenase-encoding olsD gene from Burkholderia cenocepacia causes the hydroxylation of the amide-linked fatty acyl moiety of ornithine-containing membrane lipids". Biochemistry. 50(29):6396-6408. [doi:10.1021/bi200706v]21707055
2010Davila-Martinez, Y, Laura Ramos-Vega, A, Contreras-Martinez, S, Encarnacion, S, Geiger, O, Lopez-Lara, I. (2010). "SMc01553 is the sixth acyl carrier protein in Sinorhizobium meliloti 1021". MICROBIOLOGY-SGM. 156(1):230-239. [doi:10.1099/mic.0.033480-0]19797355
2000de Rudder, K, Lopez-Lara, I, Geiger, O. (2000). "Inactivation of the gene for phospholipid N-methyltransferase in Sinorhizobium meliloti: Phosphatidylcholine is required for normal growth". Molecular Microbiology. 37(4):763-772. [doi:10.1046/j.1365-2958.2000.02032.x]10972799